查询词典 enzyme-linked immunosorbent assay

32 U/mL enzyme activity can be detected in medium after fermentation. By optimizing the zymotic medium and fermentation conditions, recombination B. subtilis can express as high as 110.02 U/mL of Alkalineβ-mannanase. Cycle of fermentation needs 5 days(4 days is needed in induced expression ), then enzyme activity maintained at the highest level, less than 5 % enzyme activity was lost in next 2 days.

为了继续对碱性β-甘露聚糖酶进行高表达研究，试验采用了芽孢杆菌发酵培养基来进行产酶研究，初步诱导酶活达到29.32 U/mL，通过对发酵条件的优化，该重组枯草芽孢杆菌的产酶水平有了大幅度的提高，最高可达110.02 U/mL。

However, the first one enzyme 1-deoxy-D-xylulose 5-phosphate synthase gene and the second one enzyme 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene in MEP pathway had been reported and expressed in the latex of rubber tree, indicating that MEP pathway may be involved in rubber biosynthesis.4 - hydroxy -3 - methyl -2 -- butenyl -4 - phosphate reductaseis the last enzyme that catalytic 4 - hydroxy -3 - methyl -(2E)- butenyl -4 - phosphate to generate isopentenyl pyrophosphate.

然而已有报道MEP途径中第1个酶1-脱氧-D-木酮糖-5-磷酸合成酶（1-deoxy-D-xylulose 5-phosphate synthase，DXS）基因和第2个酶1-脱氧-D-木酮糖-5-磷酸还原酶（1-deoxy-D-xylulose 5-phosphate reductoisomerase ，DXR）基因在橡胶树胶乳中的表达，这表明MEP途径可能参与橡胶的生物合成。4-羟基-3-甲基-2--丁烯基-4-磷酸还原酶（4-hydroxy -3-methyl-2--butenyl-4-diphosphate reductase，HDR）是异戊烯基焦磷酸合成途径之一甲基赤藓糖磷酸（methylerythritol phosphate，MEP）途径中的最后一个酶，催化4-羟基-3-甲基-(2E)-丁烯基-4-磷酸生成异戊烯基焦磷酸。

The experiment two wasundertaken to examine the effect of different enzyme preparation levels on growthperformance, nutrient digestibility, intestinal morphology, serum biochemical parameters,physico-chemical property and chemical digestion of intestine digesta, endogenousdigestive enzyme and caecal microbial population for gosling, the diet with stuff of highfiber content was added 0, 0.2% and 0.4% enzyme preparation for the experimentalgroups.

试验二采用单因素试验设计，研究含高纤维原料的日粮中添加不同酶制剂水平(0、0.2％、0.4％)对雏鹅生长性能、养分消化率、消化道组织形态、血清指标、肠道食糜理化性质和化学性消化、机体内源性消化酶及盲肠微生物等的影响。

The effects of cadmium stress on POD activity of D. caryophyllus were mainly on the change of individual enzyme belt Rf value, the drop of enzyme band number, and the increase of enzyme activeness.

镉胁迫下对石竹叶POD的影响主要表现为个别酶带Rf值的改变、酶带数的下降和活性的增加。

It has been reported that F185K and C280S mutations of HIV1 integrase would improve the enzyme solubility, and the catalytical activity of the enzyme was the same as that of the wildtype enzyme in vitro.

coli中进行整合酶基因表达，SDSPAGE鉴定表达产物，亲和层析纯化蛋白，酶联免疫吸附实验方法测定整合酶的生物学活性。

Compared with prozyme A, alkali enzyme was a powerful enzyme for the hydrolysis of corn protein, but it could result deamidation, that was another reason for selecting prozyme A as the first enzyme.

与复合酶A相比，碱性蛋白酶也能较好地作用于黄粉蛋白，但它对酰胺基有较强的水解作用，这是选择复合酶A作为第一次水解用酶的又一个主要原因。

The result of immobilization showed that hydrophile membranes were more advantageous than hydrophobe ones to act as immobilization carrier. Crosslinking organophosphorus hydrolase by glutaraldehyde on polyethersulfone membrane is a simple and practical immobilization method. An appropriate amount of bovine serum albumin and crosslinker is necessary to get a good result of immobilization. In the biodegradation of methyl-parathion through enzyme membrane reactor, the effect of immobilized enzyme amount, flow rate of peristaltic pump, pH of the feed, and the methyl-parathion concentration on biodegradation rate was studied. It was shown that the biodegradation rate increased with immobilized enzyme amount and substrate concentration. Biodegradation rate didn't increase when the folw rate of the peristaltic pump was larger than 7 ml/min.

然后，研究固定化酶降解甲基对硫磷，从不同材料的膜载体固定有机磷水解酶的比较中，发现亲水膜比疏水膜适合作固定化酶载体；在此基础上，将有机磷水解酶固定在聚醚砜微滤膜上，并制成酶膜生物反应器，用于降解甲基对硫磷；戊二醛化学交联法固定时，酶液与10%牛血清白蛋白溶液的比例在3：1~2：1，与交联剂的比例在7：2~7：4范围内，固定化效果较好，并发现牛血清白蛋白和膜载体有降低酶活损失的作用；将固定化的酶膜装于酶膜反应器降解甲基对硫磷，实验结果表明：反应器的降解速率与固定化酶量呈正比，也随底物浓度的增加而加快，当流速低时，降解速率随流速增大而加快，到7ml/min以上时，降解速率不随流速脑黾佣洌到獾淖畹团ǘ任？

In this study, we investigate the heat-induced inhibition of drug-metabolizing enzyme activity in mice fed with freeze-dried, heated freeze-dried or half amount autolyzed freeze-dried Brussels sprouts powder. 0.5% ethoxyquin diet was fed to another group of mice as a positive control. The study mice, four per group, were fed a diet consisting of 20% Brussels sprouts for 7 days. Each group had its own pair-fed group. We found significantly greater inductions of glutathione S- transferase, quinine reductase, and phase Ⅱ drug-metabolizing enzyme activities in groups fed within freeze-dried and heated freeze-dried Brussels sprouts than their pair-fed groups. These findings indicate that intestinal microflora still can hydrolyze glucosinolates without plant myrosinase activity, though the alteration of cytochrome- P-450 1A (CYP1A, a phase Ⅰ drug-metabolizing enzyme) induction was only 1.4-fold.

本实验欲进一步探討加热处理之抱子甘蓝对phase Ⅰ与phase Ⅱ解毒酵素活性之影响差异，实验分三组，抱子甘蓝(1)冷冻乾燥组（glucosinolate未裂解）(2)加热冷冻乾燥组(3)半量之裂解冷冻乾燥组（glucosinolate已裂解），(4)and 0.5% ethoxyquin diet was used as a positive control以2%重量混合成饲料，餵以小白鼠（4只／组）7天，结果发现，在第(2)组小白鼠GST phase Ⅱ酵素）活性显著上升，显示在植物裂解酵素不存在之状况下，肠胃道之微生物仍可促进裂解反应，但由CYP1A活性增加不显著（仅1.4倍）看来，根据定义：单独phase Ⅱ酵素活性增加，为monofunctional induction/inducer，可加速致癌物之代谢。

Compared with thermozyme,the first-class structure change of the psychrophilic enzyme can lead to space structure change and the structure of the enzyme is more flexible.Thus,this enzyme can catalyze with less energy in low temperature.

与嗜温酶相比，嗜冷酶一级结构的变化导致空间结构的变化，使酶分子的构象具有更大的柔性，同时也有利于这类酶在低温条件下需要更少的能量而发挥催化作用。

Study on 6 soil enzyme activities and 7 siol fertility factors of 12 different planting modes,analyse the dates with the correlation analysis and the canonical correalation analysis,In order to reveal the relations between the soil enzyme activities and soil fertilities in the low hill lands along Yantze River , The result of the correlation analysis showed that they had a great relation between the soil enzyme activities and siol fertility factors.

对12种不同种植模式的6个土壤酶活性和7个土壤肥力因子进行测定，运用相关分析和典型相关分析方法对数据进行分析，以期阐明沿江丘陵地区土壤酶活性与土壤肥力因子的关系。相关分析的结果表明土壤酶活性与土壤肥力因子密切相关，多数呈极显著正相关。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。