查询词典 enzyme-linked immunosorbent assay

The method of enzyme-linked immunosorbant assay was employed to determine the contents of indoleacetic acid, abscisic acid, gibberellin (GA3) and Zeatin under pot culture.

在盆栽基质培养条件下，进行了打顶和打顶+生长调节剂处理，采用酶联吸附免疫测定技术分析了烤烟根和叶中内源IAA、ABA、GA3和ZR的变化。

Mature and immature embryos of maize inbred line 87-1 were used as explants and calli were induced and cultured on improved N6 medium. The method of enzyme-linked immunosorbant assay was employed to detect endogenous contents of abscisic acid, indoleacetic acid, gibberellins (GA3) and cytokinins of immature, mature embryos and their calli during culture process.

以玉米自交系87-1的幼胚和成熟胚为材料，采用酶联免疫法测定了不同培养时间诱导的幼胚及成熟胚愈伤组织内源脱落酸、吲哚乙酸、赤霉素(GA3)和细胞分裂素含量的动态变化。

The methods of enzyme-linked immunosorbant assay were employed to detect endogenous contents of abscisic acid, indoleacetic acid, gibberellins (GA3, GA4) and cytokinins of immature embryos, embryonic and non-embryonic calli during culture process.

酶联免疫法测定不同培养时间后未成熟胚，以及由此诱导的胚性和非胚性愈伤组织，内源脱落酸、吲哚乙酸、赤霉素(GA3、GA4)和细胞分裂素(DHZR、IPA、ZR)含量。

The myocardial enzyme, liver duty enzyme, kidney duty enzyme, amylopsin and blood sugar in serum were detected, the results showed that CK, CK-MB, ALT and AMY had different extent changing characteristic, but CRE and BUN no obviously change.

分别于接种病毒后15天和30天剖杀猕猴进行病理学组织学观察，其病变以病毒性心肌炎、间质性肾炎、神经系统的非化脓性炎症及肝脏汇管区有细胞增生性反应为主要特征，其他组织器官也有不同程度的病理变化。

This article focus on enzyme gene clone and heterogenesis expression,site directed modify and orthogenesis,fu-sion protein and fusion enzyme,enzyme mimics(abzyme and molecular imprinting)and telomere .

本文着重介绍了酶基因克隆与异源表达、酶分子的定向改造和进化、融合蛋白与融合酶、酶的人工模拟和端粒酶，综述了分子酶工程的研究进展、趋势及其应用。

But pancreatin is a mixtrure of various enzymes, each enzyme has a low purity and many enzymes have similar molecule structure and physicochemical properties, when extracting one enzyme, it is easy to abandon the otherone as impurity. This is why there are few technoloies of stimutaneous extraction of more than three enzyme from pancreatin and all the technoloies have shortages.

但是由于胰酶为多种酶的混合物，每种酶的纯度很低，许多酶又具有相似的分子结构和理化性质，因此往往在提取一种酶的同时，其它酶被当作杂质处理掉了，这就导致了胰酶中三种以上酶联产的工艺极为少见，而且已有工艺都有各自的不足。

Making standard curve by salicin enzyme activity ,we can get the formula of relative and absolute enzyme activity ,then transform into absolute enzyme activity of glucose.

通过水杨苷法作葡萄糖标准曲线，能得到相对酶活与绝对酶活的换算公式，进而得到绝对酶活。

The purified recombinant enzyme had optimal reaction conditions at pH 5.0 and 80 °C, was stable between pH 4.0 and pH 6.0. The enzyme had residual activity of 60% at 95 °C for 6 h at pH 5.0, thus has considerable potential in industrial applications. The specific activities of the enzyme to salicin, p-Nitrophenyl β-D-glucopyranoside, p-nitrophenyl β-D-xylopyranoside and p-nitrophenyl α-D-glucopyranoside were: 57500 U/mg, 1632 U/mg, 109 U/mg, and 39 U/mg, respectively, the specificity of salicin was the highest, p-nitrophenyl β-D-glucopyranoside followed, no activities to xylan and arabinofuranoside.

重组 Tm-SIGlA 的酶学性质研究表明：该酶最适作用温度和最适作用 pH 分别为80 ℃和 pH 5.0；在 pH 4.0-6.0 之间酶活性稳定，95 ℃保温 6 h 残留活性为 60%，具有非常高的热稳定性；在所测定的底物中，该酶对水杨苷、对硝基苯酚-β-D-葡萄糖苷(p-Nitrophenyl β-D-glucopyranoside)、对硝基苯酚-β-D-木糖苷(p-Nitrophenyl β-D-xylopyranoside)和对硝基苯酚-α-D-葡萄糖苷(p-Nitrophenyl α-D-glucopyranoside)的比酶活分别为：57500 U/mg、1632 U/mg、109 U/mg 和39 U/mg，作用水杨苷的专一性最强，其次为对硝基苯酚-β-D-葡萄糖苷，没有检测到对木聚糖和阿拉伯糖苷的活性。

Eggs and soyabean milk contains soap protein enzyme stayer,which can control liveness of flesh's protein enzyme,affecting protein digestion and absorption internal to the body.Egg white contains tough protein,which can integrate with soap protein enzyme in the soybean milk,blocking the break down of protein,thereby reducing the protein absorptivity of the body.

鸡蛋与豆浆生豆浆中含有胰蛋白酶抑制物，它能抑制人体蛋白酶的活性，影响蛋白质在人体内的消化和吸收，鸡蛋的蛋清里含有粘性蛋白，可以同豆浆中的胰蛋白酶结合，使蛋白质的分解受到阻碍，从而降低人体对蛋白质的吸收率。

It was helpful to extract enzyme by using homogenate in citrate-phosphate buffer; The enzyme activity extracted from pH5.6 buffer is higher than that of pH5.8.The loss of enzyme activity of this zymin is better, but easily used in manufacture.

丙酮粉提取法会引起回收酶活性较大损失，其总酶活仅是缓冲液匀浆法和浸提法的1/5、1/7，但酶制剂本身的酶活性较好，使用方便，有利于工业化生产应用。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。