查询词典 enzyme-linked immunosorbent assay

Parachutists Parachuting Heat stress protein Antibody titer Enzyme linked immunosorbent assay

空降兵；跳伞；热应激蛋白；抗体滴度；酶联免疫吸附试验

An enzyme linked immunosorbent assay was developed using heat stress protein 70 purified with its cDNA and pET vector system and the titer of antibody to HSP70 in plasma was determined in 40 control parachutists without parachuting for half year and 42 parachutists after parachuting.

目的：探讨HSP70抗体滴度在评价跳伞对空降兵健康效应中的影响。方法：利用cDNA和pET载体系统纯化的HSP70，建立检测HSP70抗体滴度的酶联免疫吸附鉴定方法，并调查了40名近半年来未跳伞的对照组空降兵和42名跳伞后的跳伞组空降兵血浆中HSP70抗体滴度。

Methods:In patients with ACS(n=32) and controls(n=21) the selective coronary arteriography was performed and the coronary artery stenosis scores were evaluated through accumulative total integration. Plasma concentrations of fibrinogenwere determined with hemagglutinin analysator and plasma concentrations of von Willebrand factor、 D-Dimer、 plasminogen activator inhibitor、platelet membrane glucoprotein(GMP-140) were determined with enzyme linked immunosorbent assay.

选择ACS患者32例和对照组21例进行选择性冠状动脉造影，对冠脉造影结果采用累计积分法进行冠状动脉狭窄评分；使用血凝分析仪测定受试者血浆纤维蛋白原水平，使用酶联免疫法测定外周静脉血血管性血友病因子、D-二聚体、纤溶酶原激活剂抑制物、血小板膜糖蛋白140（GMP-140）的含量。

The result of study showed:1 The effect of some inorganic urea inhibitor enzyme indicated boracic acid inhibits the activeness of urea enzyme in soil well, delayed the speed of urea hydrolysis in the soil and decreased the volatilization of ammonia , boracic acid,can be chosen as a inorganic urea enzyme inhibitor.2 The effect of some organic urea inhibitor enzyme showed that the dissociative Humic Acids of turfy soil and weathered coal inhibit the activeness of urea enzyme in soil well.

室内模拟试验，对几种缓释氮肥的释氮研究表明，和其它几种氮肥相比，PCF和PSF的控氮时间都较长。 6。盆栽试验表明，PCF的释氮效果比其它缓释氮肥的要好，且在油菜生育的各个时期，释氮都较充足，是适合油菜的良好缓释氮肥。

The main component of ramie glial was pectin, hemicellulose and lignin; The single factor on degumming of ramie test was respectively analyzed with a high vigorous of pectinase KDN and TZ-888 compound enzyme （mainly xylanase and mannanase on the pH value, bath ratio, dosage of enzyme, metal ions, temperature and time.The orthogonal test of L9（34 were done by bath ratio, dosage of enzyme, temperature and time. The results showed that the best compages of KDN pectinase on degumming was 1∶12 of bath ratio,pH 8.6,1 mmol/L Mg2+,300 IU/g of KDN pectinase,35℃、6 h;the best compages of TZ-888 compound enzyme on degumming was 1∶18 of bath ratio,pH 4.0,1 mmol/L Ca2+, 500 IU/g of TZ-888 compound enzyme,45 ℃,6 h.

摘 要：本研究根据苎麻胶质的主要成分为果胶、半纤维素和木质素，用高活性的KDN果胶酶和TZ-888复合酶（主要是木聚糖酶和甘露聚糖酶）从pH值、浴比、酶用量、金属离子、温度、脱胶时间等方面进行单因素试验，然后选取影响显著的浴比、酶用量、温度，时间进行四因素三水平的脱胶试验，结果表明鲜麻采用KDN果胶酶脱胶最佳组合为浴比1：18，pH 8.6， 1 mmoL/L Mg2+，酶用量300 IU/g，温度35 ℃、时间6 h时处理，TZ-888复合酶脱胶最佳组为浴比1：12，pH 4.0， 1 mmoL/L Ca2+，酶用量500 IU/g，温度45 ℃，时间6 h进行脱胶为优化的试验条件。

The main component of ramie glial was pectin, hemicellulose and lignin; The single factor on degumming of ramie test was respectively analyzed with a high vigorous of pectinase KDN and TZ-888 compound enzyme （mainly xylanase and mannanase on the pH value, bath ratio, dosage of enzyme, metal ions, temperature and time.The orthogonal test of L9（34 were done by bath ratio, dosage of enzyme, temperature and time. The results showed that the best compages of KDN pectinase on dry ramie degumming was 1∶12 of bath ratio,pH 8.6,1 mmol/L Mg2+,20 IU/g of KDN pectinase,4℃、4h;the best compages of TZ-888 compound enzyme on degumming was 1∶18 of bath ratio,pH 4.0,1 mmol/L Ca2+, 300 IU/g of TZ-888 compound enzyme,40 ℃,5 h.

摘 要：本研究根据苎麻胶质的主要成分为果胶、半纤维素和木质素，用高活性的KDN果胶酶和TZ-888复合酶（主要是木聚糖酶和甘露聚糖酶）从pH值、浴比、酶用量、金属离子、温度、脱胶时间等方面进行单因素试验，然后选取影响显著的浴比、酶用量、温度，时间进行四因素三水平的脱胶试验，结果表明干苎麻采用KDN果胶酶脱胶最佳组合为浴比1：12，pH 8.6， 1 mmoL/L Mg2+，酶用量200 IU/g，温度45 ℃、时间4 h时处理，TZ-888复合酶脱胶最佳组为浴比1：18，pH 4.0， 1 mmoL/L Ca2+，酶用量300 IU/g，温度40 ℃，时间5h进行脱胶为优化的试验条件。

Furthermore, the effects of different metallic salts,nitrogen sources,carbon sources,temperature,pH,water content and fermentation time on the enzyme activity and the yield of the acid protease were studied and the results were as follows: both MnCl2 and CuSO4 could activate the protease while other metallic salts inhibited the protease on different levels; addition of 1 % bean flour and 1 % glucose could increase enzyme yield and enzyme activity by 71 % and 31 % respectively; the maximal yield of enzyme was achieved under the conditions of temperature at 40 ℃ and pH as 7.0; water content and fermentation time would also influence enzyme yield on different levels.

不同金属盐对该酶酶活及不同氮源、碳源、温度、pH、含水量以及发酵时间对菌株产酶的影响试验结果表明：MnCl2，CuSO4均对该酶有激活作用，其他金属盐类对该酶有不同程度的抑制作用；添加黄豆粉（1 %）和葡萄糖（1 %）可使菌株产酶酶活分别提高71 %和31 %；培养温度为40 ℃以及培养基起始pH为7.0时产酶最高；含水量和发酵时间对产酶也有不同程度的影响。

Chemical methods of qualitative assay and quantitative assay to Chufa functional component According to chemical characteristics reactivity of Chufa functional component, chemical method of qualitative assay and quantitative assay to Chufa functional component were built.

功能因子的定性定量化学分析针对该功能因子的化学特征反应，实验选用硫酸显色法、可见光比色法、吸光度测定法和沉淀法对功能因子进行定性、定量分析。

After mass chemotherapy,ScAg assay in case screen for selective chemotherapy was better than that of ScAb assay and stool examination.ScAg assay was also practice in evaluation of control effect,disease surveillance in children,and to confirm repeat treatment cases.In short,ScAg assay applied for case screen would play an important role in control program.

结论检测ScAg用于全民化疗后选择性化疗的筛查方法优于ScAb及粪便检查，适用于防治效果考核，儿童感染的监测及确定重复治疗对象，经多年应用ScAg筛查血吸虫病对达到传播控制起到重要作用。

Methods compare with sysmex-r3500 analyzer、coulter maxm analyzer、miller ocularto determine 130 ordinary samples.results f=3.87,p.05,the difference is conspicuous.each"r"is 0.7331,0.7231,0.5011,each other are beeline correlation.about sysmex-r3500 analyzer,the inter-assay cv of quality control cv=2.95%,the sample is cv=4.42%;about couter maxm analyzer,the intra-assay cv is 5.70%,intra-assay cv is 17.02%;about miller ocular,the inter-assay cv is 26.15%.conclusion sysmex-r3500 analyzer is good at repeatability,convenience,quickness,so it is adapt to clinical batch samples quickly determine.

分别用sysmex-r3500分析仪，coulter maxm分析仪以及国际血液标准化委员会所推荐的miller窥盘法进行比较，对130份正常人标本进行网织红细胞测定。结果：f=3.87，p.05，差异有显著性。其两两之间r值分别是0.7331，0.7231，0.5011，两两之间直线相关。sysmex-r3500分析仪的质控物批内平均cv=2.95%，标本批内平均cv=4.42%，批间平均cv=3.42%；coulter maxm分析仪批内平均cv=5.70%，批间平均cv=17.02%；miller窥盘法批内平均cv=26.15%。结论：sysmex-r3500分析仪具有重复性好，简便，快捷的特点，尤其适合临床大批标本的快速测定。网织红细胞 miller；窥盘法；仪器法

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48