查询词典 enzyme-linked immunosorbent assay

An ELISA(enzyme-linked immunosorbent assay.) was developed for detection of bovine brucellosis by using LPS as antigen purified by Heating - phenol recommended by OIE (office international des epizootics).

采用世界动物卫生组织推荐的热酚法提取的脂多糖作为抗原，建立了牛布氏杆菌病的ELISA方法，对45份血清进行检测，证明本ELISA方法灵敏性较高，经阻断试验和交叉试验，证明特异性较好。

The concentration of PA1b in several plants including pea, taro, ginger, tomato, bitter gourd, garlic, onion, towel gourd and cucumbers was measured by competitive inhibiting enzyme-linked immunosorbent assay method to study the role of pea albumin 1b(PA1b) during the development of plants.

为研究豌豆胰岛素PA1b在植物生长发育中的作用，利用竞争抑制ELISA法，检测PA1b在豌豆、芋头、姜、西红柿、苦瓜、蒜头、洋葱、丝瓜、黄瓜等植物中的分布，以及PA1b在豌豆种子发芽过程中的含量变化。

In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

ObjectiveTo investigate the diagnostic value of glucose-6-phosphate isomerase detected by an enzyme linked immunosorbent assay in patients with rheumatoid arthritis.

类风湿关节炎(Rheumatoid Arthritis，RA)是一种常见的以关节慢性炎症为主要表现的系统性风湿性疾病，致残率高，严重影响患者的生活质量。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

These expression vectors induced robust immune responses mediated by CD4 and CD8 cells, as well as significant antibody titres, measured by enzyme-linked immunosorbent assay.

免疫了这些S抗原的DNA表达载体使小鼠肺内的病毒复制下降了六次方多而且免疫保护是由体液免疫而不是T细胞依赖的免疫机制产生的。

Serum HGF were detected by a "sandwich" enzyme-linked immunosorbent assay in 139 patients with viral hepa- titis and 20 normal subjects, TGF-β1 activity were determined by the modified growth inhibition of MV1LU cells with MTT. some liver function, fibrogeneis markers and AFP concentrations were also meas- ured at the same time.

选139例病毒性肝炎患者，以双抗体夹心ELISA检测sHGF，以改良MV1LU细胞生长抑制MTT法检测TGF－β＿1活性，同时检测其肝功能、肝纤维化、甲胎蛋白等指标。

The monoclonal antibody-based indirect enzyme-linked immunosorbent assay was used to determine the relationships among the detectable time and taken amount of white-backed planthopper Sogattella furcifera in the gut of Pardosa pseudoannulata Boes.et Str.

应用单克隆抗体研究了白背飞虱在拟环纹豹蛛体内的可测定时间与温度和捕食量的关系。

Objective To compare characteristics of treponema pallidum antibody screening method with the toluidine red unheated serum test, the enzyme-linked immunosorbent assay and the T.

目的比较无偿献血者梅毒抗体筛查方法甲苯胺红不加热血清试验、双抗原夹心法的酶联免疫吸附试验和梅毒螺旋体明胶凝集试验的特性。

Crohn's disease was induced by trinitrobenzene sulfonic acid in the model group. The mice were sacrificed 5 days afterwards and LPMC were isolated and cultured. The expression of CCR2 and CCR5 on LPMC were determined by flow cytometry. The secretion of MIP-1α in the supernatant was determined by enzyme-linked immunosorbent assay, and the expression of MIP-1α mRNA was determined by reverse transcriptase polymerase chain reaction.

模型组小鼠以三硝基苯磺酸灌肠造模。5d后处死，提取和培养LPMC，采用流式细胞仪测定小鼠LPMC中趋化因子受体CCR2、CCR5的表达；采用酶联免疫吸附测定检测培养上清液中MIP-1α的分泌；采用逆转录聚合酶链反应检测MIP-1α mRNA的表达。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48