查询词典 enzymatic

Taking loquat as material, the enzymatic hydrolysis technology was used to improve the juice yield. Using screening superior acetobacter for the fermention of loquat wine and the Loquat vinegar acetification technique with carrier adsorption, the acid-producing rate could reach more than 85%, combinedwith the technique of nonthermal removal bacteria and the technique of rapid artificial vinegar aging, the quantity of Loquat vinegar was improved.

以枇杷为原料，采取酶解技术提高出汁率；以筛选的适宜菌株进行枇杷的酒化及采用固定化高活力醋化，乙酸得率可达85%以上，结合使用非热除菌保藏、快速人工催陈技术，提高枇杷果醋的品质。

The main collagens in skin are type I and III. In this research, total collagens were separated from rat skin by enzymatic digest and acetum methods. The collagens were denatured at 60℃ and digested with trypsin. Characteristic peptides typical for collagen type I and III were identified with high performance liquid chromatography/mass spectrometry.

哺乳动物皮肤真皮中胶原蛋白含量约为70%，主要为是I型、III型胶原蛋白，本实验利用稀酸溶解和酶法提取了大鼠皮肤中的总胶原蛋白，将胶原蛋白粗提品在60℃变性后用胰蛋白酶进行降解，液相色谱/质谱联用法分析了两种胶原蛋白的特征多肽，利用特征多肽比较了不同生长期大鼠皮肤中I型和III型胶原蛋白相对含量。

The experiment results showed that the relative growth rates, feed modulus, protein reservation rate, contents of magnesium and small peptides in plasma on grass carps in 0.5% enzymatic casein group were significantly higher than those in 0.5% casein group and 0.5% acidify hydrolyzed casein group p

草鱼饵料中添加一定比例的小肽可提高饲料表观消化率和蛋白消化率，增加血液循环中生物活性肽的含量，减少肝、胰脏和肠系膜脂肪储积，提高机体对饵料中氨基酸的利用率，从而增加体内氮沉积，提高生长速度。

The diets of test group 1, 2 and control group included in 0.5% enzymatic casein (small peptides above 80% and average length of peptides is 2.98), 0.5% acidify hydrolyzed casein(free amino acids above 80%) and 0.5% casein.

试验期56天。结果表明：添加O.5％酶解酪蛋白组草鱼其相对生长率、净蛋白沉积率、饲料系数和血浆中镁含量与小肽总量均显著高于添加0.5％酪蛋白组和0.5％酸解酪蛋白组(p＜0.05)。

In addition, both reaction time and temperature also have effects on enzymatic treatment.

另外，酶处理温度和时间也影响酶作用效果。

Firstly, the preparation of DG by enzymatic glycerine alcoholysis of palm oil was studied.

试验同时发现，体系中游离脂肪酸生成速率较快，尤其在前12小时。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Ascorbate acts as an important antioxidant in both enzymatic and non-enzymatic reactions in plant cells.

抗坏血酸作为一种重要的抗氧化剂，在植物细胞的酶促和非酶促反应中都具有重要意义。

These enzymatic haemostatic agents can be used to antagonize the side effect of hirudin. We take the batraxobin as an example to illustrate that the enzymatic haemostatic can antagonizes hirudin. Agglutination reaction occurred after the tubes containing hirudin were added with batroxobin , i.e., batroxobin can antagonize hirudin. In a canine model,, the reduction extent of TT and APTT in the test group was larger than that in the control group; the bleeding time was decreased obviously and it was shorter in the experimental group than in the control group at lh, 2h and 3h after hirudin injection, indicating batroxobin can antagonize the bleeding side effects of hirudin.

以巴曲停为例，加入巴曲停的水蛙素抗凝试管内又可发生凝集反应，而未加入巴曲停的试管内不发生凝集反应；犬动物模型显示，水蛙素实验组TT值、 APTT值明显低于水蛙素对照组，犬给予巴曲停后出血时间明显缩短，出血时间在滴注水蛙素后lh、2h、3h明显短于对照组(P.05)，说明巴曲停有拮抗水蛙素抗凝血的作用。

Soybean Polyphenol Oxidase Soybean Peroxidase Enzymatic Activity Enzymatic Heat Stability Inhibiting effect Soybean lecithin Dimercaprol

大豆多酚氧化酶；大豆过氧化物酶；酶活力；酶热稳定性；大豆卵磷脂；二巯基丙醇

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。