查询词典 enzymatic

The bioreaction mechanism and kinetic behavior of protein enzymatic hydrolysis in active peptides preparation were investigated to model and characterize the enzymatic hydrolysis curves.

研究蛋白质酶促水解制备生物活性多肽的反应机理与动力学行为，并建模表征不同反应机制的酶解曲线。

Want the skin to still can produce new collagen only, cuticular collagen is enzymatic build in responsible compose crudely collagen while also adjust of skin collagen form; Cut generation collagen is enzymatic.

只要皮肤还能产生新的胶原质，表皮胶原酶就在负责构建未成熟的胶原质的同时也调节皮肤胶原质的形成；伤口产生胶原酶。

The production of GEON included the enzymatic hydrolysis of corn protein and the separation and concentration of GEON from the resulted corn protein hydrolyzate. The enzymatic hydrolysis of corn protein needed two enzymes.

Gln活性肽的生产工艺包括酶解、分离和浓缩等单元操作，通过试验确定用两次酶解工艺。

Results It showed that the serum active enzymatic of 5′-NT in hepatic and gall diseases,osteal diseases levels were increase, especially the more significant increase of the serum active enzymatic of 5′-NT in level appeared in the patients with hepatic and gall diseases,osteal diseases .

目的 检测各型肝胆疾病、骨骼疾病患者血清5′-核苷酸酶(5′-NT)，谷丙转氨酶和γ-谷氨酰转肽酶，碱性磷酸酶酶活性的变化，观察在肝胆疾病和骨骼疾病中的临床意义，同时与正常健康者作对照。

Results It showed that the serum active enzymatic of 5′-NT in hepatic and gall diseases,osteal diseases levels were increase, especially the more significant increase of the serum active enzymatic of 5′-NT in level appeared in the patients with hepatic and gall diseases,osteal diseases .

结果 发现在肝胆疾病和骨骼疾病时，血清5′-NT酶活性升高，尤其是急性黄疸型肝炎、肝癌、胆道疾病患者血清5′-NT酶活性差异有非常显著性（ P ＜0.01）时，随病情好转，血清5′-NT酶活性有所降低。

Results It showed that the serum active enzymatic of 5′-NT in hepatic and gall diseases,osteal diseases levels were increase, especially the more significant increase of the serum active enzymatic of 5′-NT in level appeared in the patients with hepatic and gall diseases,osteal diseases .

作者：李顺君，黄文方，饶绍琴目的检测各型肝胆疾病、骨骼疾病患者血清5′-核苷酸酶(5′-NT)，谷丙转氨酶和γ-谷氨酰转肽酶，碱性磷酸酶酶活性的变化，观察在肝胆疾病和骨骼疾病中的临床意义，同时与正常健康者作对照。

The article basically elaborates ADAM familial the relation with tumor. 1ADAM gene structure and functional ADAMs are familial it is an important metallic albumen enzymatic familial, belong to albumen of zincic dependence metal enzymatic super- familial, cent is two groups:っ狝 DAMs and secrete model AD-AMTSs.

本文主要阐述ADAM家族和肿瘤的关系。1ADAM基因结构和功能ADAMs家族是1个重要的金属蛋白酶家族，属于锌依靠性金属蛋白酶超家族，分为两组：膜锚ADAMs和分泌型AD-AMTSs。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Ascorbate acts as an important antioxidant in both enzymatic and non-enzymatic reactions in plant cells.

抗坏血酸作为一种重要的抗氧化剂，在植物细胞的酶促和非酶促反应中都具有重要意义。

These enzymatic haemostatic agents can be used to antagonize the side effect of hirudin. We take the batraxobin as an example to illustrate that the enzymatic haemostatic can antagonizes hirudin. Agglutination reaction occurred after the tubes containing hirudin were added with batroxobin , i.e., batroxobin can antagonize hirudin. In a canine model,, the reduction extent of TT and APTT in the test group was larger than that in the control group; the bleeding time was decreased obviously and it was shorter in the experimental group than in the control group at lh, 2h and 3h after hirudin injection, indicating batroxobin can antagonize the bleeding side effects of hirudin.

以巴曲停为例，加入巴曲停的水蛙素抗凝试管内又可发生凝集反应，而未加入巴曲停的试管内不发生凝集反应；犬动物模型显示，水蛙素实验组TT值、 APTT值明显低于水蛙素对照组，犬给予巴曲停后出血时间明显缩短，出血时间在滴注水蛙素后lh、2h、3h明显短于对照组(P.05)，说明巴曲停有拮抗水蛙素抗凝血的作用。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48