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By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome. The whole fragment was sequenced by dideoxided chain termination methods and a full open reading frame which encode 299 amino-acids was found. The molecular weight of the peptide is about 35 kilodalton.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段,用双脱氧链终止银染测序法测定了该片段的全序列,发现它含有一个完整的开放阅读框,编码299个氨基酸残基的多肽,分子量约35KD。

Our previous studies have shown that ubiquitous overexpression of HmgD caused hemocytes proliferation and the formation of melanotic tumors in the Drosophila larvae. To clarify the mechanism of melanotic tumors appearance,we also generated RT-PCR analysis to test the transcriptional changes of PPAE and Spn27A,which encode the key factors in the phenoloxidasecascade pathway from larvae samples of mid-third instar,late-third instar and prior to pupation relative to wild type flies.Our result showed that in the HmgD overexpression mid-third instar larvae,the expression level.of Spn27A was lower than wild type,otherwise the expression level of PPAE was higher than wild type.It suggests that overexpression of HmgD affects the PO cascade pathway.

本研究组前期工作发现,过量表达HmgD引起果蝇幼虫体内血细胞增生,并出现黑色素瘤,为探讨HmgD过量表达导致了血细胞增生即形成黑色素瘤的分子机制,本研究分别提取三龄中期、后期和相对于野生型化蛹前时刻的幼虫的RNA,首先检测了编码酚氧化酶级联反应途径中的两个关键基因PPAE,Spn27A在突变体中的转录发生变化,结果发现,Spn27A在三龄中期的表达量低于野生型,而PPAE的表达高于野生型,表明过量表达HmgD影响了酚氧化酶级联反应途径,低水平的Spn27A不足以抑制PPAE的活性,活化的PPAE催化了PPO的活性,使其转变成有活性的PO,从而促进黑色素的生成,使果蝇体内产生黑色素瘤。

We initiated a genetic screen for Drosophila olfactory genes by means of SG18.1-Gal4/UAS system: The result validates our screen as an rapid, effective approach for recovering genes controlling glomerular map patterning; From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes; These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling.

本文首次利用SG18.1-Gal4/UAS基因筛选系统对果蝇嗅觉基因进行了筛选和鉴定:结果表明,所采用的方法可以快速、有效地分离到果蝇嗅觉相关基因;从1515株果蝇P{GS}品系分离到86株突变体,其中40株有明显表型,从中鉴定了23个基因,这些基因在ORN强迫表达时可以损坏果蝇嗅叶的固有结构;在筛选中发现的一些调节嗅觉图谱发育的基因可以编码新蛋白或编码参与轴突生长和细胞骨架重塑的蛋白;为了便于研究,根据基因的功能和所编码蛋白的特点,我们对分离到的基因进行了分类,即参与轴突引导和突触产生、调节转录、调节细胞骨架和功能未确定或未知的基因;并通过对嗅觉感觉器的检测发现,除了调节转录的基因外,其他基因对触觉器的数量和分布影响不明显。

Likewise, a monadic programming style enables us to encode exceptions and state in a legible, compositional manner.

类似地,单质编程风格帮助我们用一种清晰的、组合的方式来描述异常和状态。

Wnt7a and Wnt7b encode two Wnt ligands produced by the neuroepithelium of the developing CNS coincident with vascular invasion.

Wnt7a和Wnt7b能够编码由与血管侵入保持一致的发育中枢神经系统产生的两种Wnt配合基。

Niemann-Pick disease type C is a fatal, neurovisceral storage disease neuropathologically characterized by cytoplasmic sequestration of cholesterol in neurons and progressively widespread neuronal loss, caused by mutations in genes NPC1 and NPC2 (also HE1) which encode for proteins required for the intracellular trafficking of cholesterol from lysosomes to late endosomes.

C型Nimann-Pick病是一种致命性的神经内脏脂质沉积病,其主要病理特征为广泛、进行性的神经元脱失,它由分别位于18号和14号染色体上编码与细胞内转运胆固醇有关蛋白质的NPC1和NPC2基因突变所致。

MRS2 encode a magnesium transporter protein in Saccharomyces cerevisiae and deletion of MRS2 gene results in decrease in mitochondrial magnesium concentration, Group II RNA splicing defect and growth defect on nonfermentable carbon source.

MRS2 基因编码线粒体镁离子转运蛋白,MRS2缺失会导致酵母线粒体镁离子浓度下降、线粒体内II型内含子剪接缺陷和非发酵碳源上的生长缺陷。

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

近年来脑胶质瘤的基因治疗发展迅速,应运而生的方法有自杀基因、免疫基因、多药耐药基因以及抗血管生成基因等,其中自杀基因被认为是最有前景的基因治疗方法,它又称病毒介导的酶/药物前体疗法,是利用转基因技术将哺乳动物细胞中所不含有的自杀基因转入到哺乳动物肿瘤细胞中,该基因表达的产物可将无毒的药物前体转化为毒性药物,从而选择性杀伤该肿瘤细胞,常用的自杀基因有单纯疱疹病毒-胸苷激酶基因和大肠杆菌胞嘧啶脱氨酶基因,前者催化无毒性抗病毒核苷类似物如丙氧鸟苷、无环鸟苷等成为单磷酸核苷衍生物,然后在内源性细胞激酶作用下转化为具有明显毒性的三磷酸核苷,作为DNA合成链的终止剂和DNA合成酶的抑制剂,干扰细胞DNA的合成;后者编码的胞嘧啶脱氨酶可催化5-氟胞嘧啶(5-FC)脱氨成为5-氟尿嘧啶(5-FU),然后代谢为有毒性的5-氟尿嘧啶-5′三磷酸(5-FUTP)和5-氟-2′脱氧尿嘧啶-5′磷酸(5-FdUTP),5-FUTP通过与UTP竞争性结合而抑制mRNA和tRNA的合成,5-FdUTP则作用于胸苷合成酶,导致TMP衰竭而阻止DNA的合成,最终诱导肿瘤细胞凋亡。

The discoveries of the hemoglobin genes which encode the hemoglobins related to symbiotic nitrogen fixation in legumes and those not related to symbiotic nitrogen fixation in legumes andnon-legumes including cereals , make reseajrchers have a new look, resulting in two kinds of planthemoglobins: symbiotic and nonsymbiotic hemoglobins.

对这两种血红蛋白的性质、功能、基因结构及表达等方面的研究不仅对共生固氮中植物与微生物的相互关系和固氮工程研究;而且对植物细胞的呼吸代谢和耐涝机理等研究有重要价值。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白,后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾,编码RNA聚合酶,含有微小RNA病毒RNA聚合酶的八个保守基序,同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

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我比喻得过头了。