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Observations on embryonic stage and nuclear number The embryonic development stage of encysted embryos of four Artemia populations from Qinghai-Tibet Plateau was studied with paraffin section method, and the nuclei numbers of encysted embryos were estimated by counting nuclear on squashed preparations.

不同种群卤虫卵卵壳结构的观察比较利用扫描电镜和透射电镜对七个种群(GH、AQK、山西解池SIN、山东埕口盐场CK、西藏、新疆鲸鱼湖、西藏拉果错湖LGC)的卤虫卵的卵壳结构进行观察。

MethodsHuman embryonic lens epithelial cells were cultured by the explanting culture methods. An inverted phase contrast microscope, electron microscope and immunocytochemistry were used to study the growth specialties of the human embryonic lens epithelial cells.

方法组织块贴壁法培养人胚晶状体上皮细胞，通过倒置相差显微镜、透射电子显微镜，采用免疫细胞化学法分析人胚晶状体上皮细胞的生长特性。

To isolate, cultivate and identify the human embryonic fibroblasts derived from the gonadal ridges and dorsal mesenteries of human embryos, and to detect the expression by hEFs of cytokines crucial for the growth of human embryonic germ cells in vitro.

目的：从人胚胎生殖腺嵴和肠背系膜中分离、培养及鉴定人胚胎成纤维细胞，检测hEFs表达人胚胎生殖细胞体外生长所需的重要细胞因子。

Methods Primary human embryonic germ cells were obtained by tissue culturing of embryonic genital ridges and mesenteries from the embryos aged 8 weeks, and then were passaged on the 8th and 16th day using pick-up and digestive passage method. Three kinds of digestive juice were used.

取8周人胚胎的生殖腺嵴和肠背系膜，进行组织块体外培养人胚胎生殖细胞，选择原代培养第8天和第16天的细胞，采用挑取传代法和消化传代法，其中的消化传代法采用三种不同的消化液进行传代培养，记录各代培养的细胞集落数。

The argues about ethical aspects aroused by embryonic stem cells seem to be claming down, a new argue about embryonic stem cell-derived ga metes and its ethical impact comes out.

胚胎干细胞在伦理学界引发的争论尚未平息，胚胎干细胞源性配子的诞生又在更深层次对伦理学产生了新的冲击。

Methods: To use ECM Gel as a tissue culture substrate, 3-dimention co-culture of the choroids plexus or the Neuro-2A cells with the ventral part of the embryonic spinal motor column (Embryonic 12 days, E12) were made in the substrate.

采用ECM Gel作为组织培养的基质底物，将脉络丛组织或者Neuro-2A细胞株和胚胎12天的腹侧脊髓运动神经柱进行三维共培养，观察脉络丛组织或者Neuro-2A细胞株对胚胎脊髓运动神经元轴突生长的影响。

In mammals, the Notch signaling pathway is critical for embryonic development, as targeted disruption of Notch1 or its downstream transcription factor RBP-J in mice resulted in disorganization of somites and delayed closure of neurotube, which finally leads to early embryonic lethality.

Notch信号途径在哺乳动物的胚胎发育中起着关键的作用。

The results suggest: 1 during the transition of gametes to zygotes, the activation of the embryonic genome appeared relatively indiscriminate; It＇s reasoned to be due to the histone-protamine exchange occurred in male pronuclei and the dramatic changes in the chromosome structure happened during the first run of embryonic DNA replication, which caused the inappropriate and un-selectively ie.

在配子到合子的转变过程中，胚胎基因的激活可能是一个相对无序的、随机的过程。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示：(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散，但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率，而且可加强卵丘细胞的扩散；(3)在现有实验条件下，在mTCM和mNCSU中添加10％pFF与在mNCSU中添加10％FCS相比可获得较高的猪卵母细胞核成熟率；(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样，但对体外受精后的早期胚胎发育影响不明显；(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

The methods of enzyme-linked immunosorbant assay were employed to detect endogenous contents of abscisic acid, indoleacetic acid, gibberellins (GA3, GA4) and cytokinins of immature embryos, embryonic and non-embryonic calli during culture process.

酶联免疫法测定不同培养时间后未成熟胚，以及由此诱导的胚性和非胚性愈伤组织，内源脱落酸、吲哚乙酸、赤霉素(GA3、GA4)和细胞分裂素(DHZR、IPA、ZR)含量。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失