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Isolation, cultivation and identification of human embryonic NSC from cerebral cortex of 7-12 week abortus. By using flow cytometry and MTT assay, the effects of various concentration of TSPG and TSPG cooperating with cytokines in NSC culture media for 3 days on proliferation of human embryonic NSC has studied. By employing immunocytochemistry assay of the expression of tyrosine hydroxylase, the effect of different dilution of TSPG and TSPG cooperating with IL-1 on induced differentiation of human embryonic NSC into dopaminergic neuron has researched.

从7～12周人工流产胚胎脑组织中分离培养人胚胎NSC，免疫细胞化学鉴定NSC特异性nestin抗原表达，在NSC的培养体系中加入TSPG，采用流式细胞术和MTT法检测不同浓度TSPG以及TSPG与EGF，bFGF联合应用对人胚胎NSC增殖的影响；经免疫细胞化学检测培养细胞酪氨酸羟化酶表达，分析不同浓度TSPG以及TSPG与IL-1联合应用对NSC定向诱导为DA能神经元的影响。

The whole embryonic developmental course results from the ordinal expression of a large number of genes according time and space. Many developmental mechanisms have been determined in the embryonic stage. Therefore, study on the regulative mechanism of embryonic development is important for elucidate principles of biologic growth, development and evolution.

整个发育过程都是大量的基因按照一定的时间、空间和次序表达的结果，其中许多调控机制在胚胎发育阶段就已经决定，因此，研究胚胎发育调控机制对于阐明生物的生长、发育和起源进化具有重要意义。

The quantitive value of DNA was 1.82% and 1.92% respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear-shap ed embryonic stage keep high level at mature embryonic stage.

非胚性细胞与胚性细胞期的量化值分别为1.82%和1.91%；在二细胞胚、四细胞胚、多细胞胚时期DNA缓慢增长，随着胚性愈伤组织的发育，DNA的含量在梨形胚时期达到高峰；成熟胚的DNA含量虽有所下降，但仍维持较高水平。

The quantitive value of DNA was 1.82%and 1.92%respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear-shaped embryonic stage keep high level at mature embryonic stage.

非胚性细胞与胚性细胞期的量化值分别为1.82%和1.91%；在二细胞胚、四细胞胚、多细胞胚时期DNA缓慢增长，随着胚性愈伤组织的发育，DNA的含量在梨形胚时期达到高峰；成熟胚的DNA含量虽有所下降，但仍维持较高水平。

Mouse embryonic stem cells can give rise to ectodermal derivatives in culture and can be further induced into neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery.

小鼠胚胎干细胞定向分化为神经细胞的研究进展论文小鼠胚胎干细胞（embryonic stem cell ， ES cell）体外培养可以分化成为外胚层组织，并进一步分化为神经元和神经胶质细胞。

Mouse embryonic stem cells can give rise to ectodermal derivatives in culture and can be further induced into neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery.

来源：中国论文服务下载 [ 07-06-18 17：33：00 ] 作者：陆建峰，宋后燕编辑：king 小鼠胚胎干细胞（embryonic stem cell ， ES cell）体外培养可以分化成为外胚层组织，并进一步分化为神经元和神经胶质细胞。

Many hES cell lines have been established under different conditions in the world. The firstly established hES cell lines were cultured on mouse embryonic fibroblast cells with medium containing many undefined animal components such as fetal bovine serum, which may cause cross-transfection with animal pathogen and mycoplasm.

目前，世界上已经建立了多株hES 细胞系，最早建立的hES 细胞系是生长在小鼠胚胎成纤维(mouse embryonic fibroblast， MEF)细胞上的，培养体系中含血清等动物源性成分，这些成分可能引起动物源性病原体或支原体的污染，从而限制了 hES 细胞的临床应用。

The study was done to compare the induction efficiency of human embryonic stem cells to neuroepithelial progenitor cells in human embryonic fibroblasts,mouse embryonic fibroblasts and feeder free systems.

中文摘要：［摘要］比较人胚胎干细胞在人胚胎成纤维细胞、鼠胚胎成纤维细胞饲养层和无饲养层3个诱导体系下形成神经上皮祖细胞的能力。

BACKGROUND: At present, studies show that a kind of stem cell community which in many kinds of organizations can differentiate into tissue cells of different embryonic layers; but those are different from embryonic stem cells, embryonic stem cell will lose the part differentiation potential gradually during the development of pregnancy, and will present some special phenotypes or the molecular markers, as CD105 and so on, will call it postembryonic pluripotent stem cells.

背景：目前研究认为在胚胎发育后的多种组织中存在一类干细胞群体，可分化为不同胚层的组织细胞；但又不同于胚胎干细胞，随着妊娠期间胚胎的发育胚胎干细胞会逐渐失去部分分化潜能，会出现一些特殊表型或分子标记，如CD105，称其为胚胎后亚全能干细胞。

The addition of malt extract and glutamine could inhibit non-embryonic calli, and make the culture be mainly composed of embryonic calli; change of the 2,4-D concentration in liquid culture medium could adjust the status of embryonic calli to the suitable developmental stage for protoplast isolation.

通过麦芽提取物和谷氨酰胺两种物质的添加，可以起到抑制非胚性愈伤生长的作用，使培养物主要以胚性愈伤为主；通过液体培养基2，4-D浓度的改变，可调节胚性愈伤处于合适的发育时期，便于原生质体的分离。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失