查询词典 embryonic cell

Plenty and purified satellite cells are obtained with these methods.IGF-1 could promote the proliferation and differentiation of mouse skeletal muscle satellite cell. Adequate single satellite cell could be obtained by frequent passaging method and the preparation are ready for for satellite cell transplanting.

结果：此方法可以获得足量、纯度较高的肌卫星细胞，适宜浓度的IGF对肌卫星细胞有较强的促增殖及促分化作用，频繁传代可以获取足量的单个肌卫星细胞，为肌卫星细胞移植做好了前期准备。

The result reveals that, The cow colostrums contains a large number of the somatic cells: SCC is 200,000 /mL, the activity of the milk somatic cell is 60% byusing Trypan blue exclusion. This somatic cell can be used to carry on the cell ulav analysis and RNA extraction, the integrality and purity quotient of the extracted RNA can be used for prefabricating cDNA.

结果：牛初乳中含有大量的体细胞（SCC为20万／mL，台盼蓝拒染测得活力为60%），可以利用提取的体细胞进行细胞分析以及RNA的提取，所提取RNA的完整性和纯度可以用来合成cDNA，看家基因GAPDH扩增条带清晰。

Methods HOS cell line was treated with various concentrations of tretinoin in different durations in vitro. Proliferation suppression was observed by MTT method and invert microscope before and after tretinoin treatment, and protracting cell growth curve. The cell growth and invasion ability was measured with the colony-formation rate in soft agar test.

不同浓度的维甲酸作用HOS细胞，采用噻唑蓝法、倒置相差显微镜观察药物对细胞生长的影响并绘制细胞生长曲线；软琼脂集落形成实验观察细胞生长和侵袭能力的变化；流式细胞仪测定细胞周期变化。

The former formed a kind of KR cyst, while the later formed a kind of NKR cyst. All the kinetosomes, ciliary shafts and microtubules were resorbed in the former, meanwhile, the cell lost most water and cell volume decreased distinctly. In the later, only part of kinetosomes and part of microtubules were resorbed. Cell lost water slightly and volume decreased slightly.

期间，细胞吸收了全部的毛基体、纤毛杆和微管结构，伴随着细胞剧烈的失水浓缩，细胞体积显著缩小；游仆虫在形成毛基体非吸收型包囊时，细胞仅经历了部分的脱分化过程，期间，细胞仅吸收了部分的毛基体及部分微管结构，并且细胞仅发生了轻微的脱水和浓缩，细胞以折叠的形式容纳在包囊壁内。

RIG-I overexpression sensitizes hepatocellular carcinoma cells to the cell arrest and apoptosis induction by IFNalphaIn addition to the critical role in the antiviral response, IFNs can also regulate the immune response, induce cell growth inhibition and cell differentiation.

三、RIG-I增强肝癌细胞对干扰素免疫治疗敏感性的研究干扰素(Interferon， IFN)作为一种病毒抑制因子，不仅具有抗病毒作用，而且还具有免疫调节、抑制细胞增殖以及诱导细胞分化等作用。

Root border cell s, previously referred as sloughed root cap cell, is a special cell population, which separates in large number from the periphery of the root cap and accumulates in the root tip.

以四季豆为研究材料，研究了四季豆根边缘细胞数目和存活率的变化及不同浓度铝对四季豆根边缘细胞的影响。

A major problem encountered in the animal cell culture bioreactor design and scale-up is that the animal cells are susceptible to damage in the presence of mechanical agitation and/or gas sparging needed to supply sufficient oxygen and mixing to the cultures. Based on the analysis of the cell damage mechanisms, here the principle of the bioreactor design for suspended animal cell cultures has been developed.

动物细胞培养用生物反应器设计和放大的关键问题是细胞破损与供氧和混合的矛盾，在分析细胞破损机理基础上，提出了动物细胞培养生物反应器的设计原理——设计模型和有关设计条件，从而清楚地确立了细胞死亡速度与培养基组成、反应器设计和操作参数间的定量关系，以及反应器设计应遵循的保证细胞生长和满足传质要求的条件。

This study was purposed to investigate the dynamic change of clonal proliferation of T cell receptor V subfamilies in peripheral blood of patients received allo-hematopoietic stem cell transplantation and to analyze the relationship between T cell clonal proliferative changes and GVHD.

本研究观察异基因造血干细施移植患者外周血T细胞受体Vβ亚家族克隆性增殖的动态变化，分析T细胞的克隆性演变与GVHD的关系。

This study was purposed to investigate the dynamic change of clonal proliferation of T cell receptor V subfamilies in peripheral blood of patients received allohematopoietic stem cell transplantation and to analyze the relationship between T cell clonal proliferative changes and GVHD.

本研究观察异基因造血干细胞移植患者外周血T 细胞受体 Vβ亚家族克隆性增殖的动态变化，分析T细胞的克隆性演变与GVHD的关系。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

The Porsche Owner: But it's my car!

可，这是我的车呀。

LOX_83}{Oh, another bunch of wackos.

{LOX_83}{呃，又一群怪人。