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The level of VEGF mRNA was elevated after 6h when 8Gy irradiation was processed and maintained to 72h. Sense and anti-sense expression vector were transfected into vascular endothelial cells and stable cell lines were established. Cell line transfected sense expression vector of VEGF_(165) gene showed reduced inhibition of prolifetation, MNF, MNCF and apoptosis compared to that of control vector, while opposite results were obtained in anti-sense transfected cell line.

转染正义表达载体的ECV-304稳定细胞系经过4Gy γ射线照射后，与转染空载体的细胞相比，细胞增殖抑制率减弱，MNF、MNCF以及凋亡减少；而转染反义表达载体的细胞系与转染空载体的细胞相比，细胞增殖抑制率增强，MNF、MNCF以及凋亡增加。

In contrast to the monolayer cell culture system, the livingness of VSMCS cultured in "three-dimensional" cell culture system had no statistical difference, but collagen restrained cell proliferation.

三维培养VSMCS与经典的单层培养相比，细胞活力无统计学差异。但细胞增殖受到一定程度的抑制。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0，5.0，10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理，设空白对照组，分别孵育24h、48h、72h后，收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化；免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

Choline and folic acid deficiencies could induce cell death in human lymphoblast cell line, and 6 μmol/L CC and 120 nmol/L FA was the optimal combination for keeping the lowest level of APO and NEC in human lymphoblast cell line.

胆碱与叶酸不足可诱导人成淋巴细胞株细胞死亡，6 μmol/L氯化胆碱及120 nmol/L叶酸是维持体外培养的人成淋巴细胞株APO及NEC频率在最低水平的最适组合浓度。

Methods The stablishment of lymphoblast cell lines was by EBV-immortalization and subtyping of the cell lines by cell fusion, mytomycin C inhibition analysis and/or immunoprecipitation and Westemn blot.

方法将我国3例FA患者外周血细胞用EB病毒活化法建立原始B-淋巴细胞系，采用丝裂霉素生长抑制试验和细胞融合后MMC抑制试验的互补试验进行分型；利用单克隆抗体免疫沉淀法和Western blot法进行分型。

They had higher SCE rate and micro nuclei rate, with 14.12times per cell, and 13.28 times per cell, respectively for SCE rate and 25.2% and 21.2%, respectively for micro nuclei rate, After treated wito mitomytin, they had even higher SCE (38.3、37.2)and micro-nuclei rate(10.7%,10.2%) than normal β－lymphoblast cell line.

现常用细胞EB病毒体外感染外周血淋巴细胞建立淋巴母细胞株，具永生性和不致瘤性，保持原有细胞的生物学特性，为实验室研究提供可信的材料[2]。

It publishes original research reports, reviews in the following areas: cell cycle regulation, cytokines, erythropoiesis, gene therapy, general hematopoiesis, granulopoiesis, hematological malignancies, immunobiology, immunotherapy, lymphopoiesis, megakaryocytopoiesis, microenvironment, monocyte development, molecular genetics, signal transduction, stem cell biology, and experimental as well as clinical stem cell transplantation.

该杂志刊载细胞周期调控、细胞因子、红细胞生成、基因疗法、全身血细胞生成、粒细胞生成、血液肿瘤、免疫生物、免疫疗法、淋巴细胞生成、巨核细胞生成、微环境、单细胞生成、分子遗传学、信号传导、干细胞生物学、实验与临床干细胞移植方面的原始论文及综述等。

Paeoniaceae,a monomarphic family,has distinctive characters for its phylogeny in Angiosperm,such as:both of tapetum and microspore mother cell are homologous,and so are nucellus cell and macrospore mother cell;there are many macrospore mother cells.

芍药科Paeoniaceae为单属科，其系统发育具有一系列的独特性，在被子植物中是独一无二的。

It turned out that many inclusions were found in the nucleolus and the cytoplasm of PK15 cell. The paracrystalline arrays was found in the inclusion in cytoplasm whose electron dense is deep; The nuclear of PK15 cell dissolved and there were margination of multiple chromatin aggregates and suspected intranuclear viral inclusion in PK15 cell.

细胞质中包涵体电子密度深，位于核膜周围，内有呈晶格状排列的病毒粒子，直径12±2nm；细胞核内染色质消散，在细胞核内发现多个电子密度深的、圆形或环状、由大量异染色质组成的包涵体，内有细微的粒状物质。

Methods OS732 was treated with 10^(-5)MATRA for 6 days, then we observed cell morphology, counted cell number, analyzed cell cycles by flow cytometry and detected mRNA expressions of cyclinD (Dl, D2, D3), CDK4, P21 by semi-quantity RT-PCR. Treatmed with 10^(-5)MATRA, The nucleus of OS732 cells became small and condensed, and cytoplasms expanded.

用10^(-5)MATRA作用骨肉瘤细胞OS732， 6d后，进行细胞形态学观察、活细胞计数、流式细胞仪分析细胞周期、半定量RT-PCR检测细胞周期调控蛋白CyclinD(D1、D2、D3)、CDK4、P21 mRNA表达的改变。

In other research, chondroitin is being investigated for use in several other conditions.

在其它研究，软骨素被调查用於几个其它情况。

They would make fun of him for having to work.

他们拿他的工作来开玩笑。