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GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

Human embryonic stem cells are pluripotent cells derived from inner cell mass of blastocyst at early stage of human embryo.

人胚胎干细胞(human embryonic stem cells，hES cells)是一种从人类早期胚胎的内细胞团中分离出来的多能性干细胞。

Objective To investigate the possibility of commitment differentiation of embryonic stem cells induced by the medium of cultured retinal neurons of SD rats.

目的 探讨视网膜神经细胞培养的上清液对胚胎干细胞(embryonic stem cells，ES)体外分化的诱导作用。

The method of embryonic body formation was used for endoderm/mesoderm commitment before the following pax4 gene transfection.

首先使胚胎干细胞形成embryonic body，四天后将EB打成单颗细胞。

Embryonic stem cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells.

胚胎干细胞(embryonic stem cell，ES cell)系是从早期胚胎细胞分离培养而建立的高度未分化的具有全能性的细胞系。

Mouse embryonic stem cells can give rise to ectodermal derivatives in culture and can be further induced into neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery.

小鼠胚胎干细胞（embryonic stem cell ， ES cell）体外培养可以分化成为外胚层组织，并进一步分化为神经元和神经胶质细胞。这些细胞已用于中枢神经系统疾病的细胞替代治疗和治疗药物的研发。

Mouse embryonic stem cells can give rise to ectodermal derivatives in culture and can be further induced into neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery.

小鼠胚胎干细胞定向分化为神经细胞的研究进展小鼠胚胎干细胞（embryonic stem cell ， ES cell）体外培养可以分化成为外胚层组织，并进一步分化为神经元和神经胶质细胞。

Mouse embryonic stem cells can give rise to ectodermal derivatives in culture and can be further induced into neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery.

小鼠胚胎干细胞（embryonic stem cell ， ES cell）体外作育可以剖析成为外胚层结构，并进一步剖析为神经元和神经胶质细胞。这些细胞已用于中枢神经体系疾病的细胞替换治疗和治疗药物的研发。

Mouse embryonic stem cells can give rise to ectodermal derivatives in culture and can be further induced into neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery.

小鼠胚胎干细胞（embryonic stem cell ， ES cell）体外培养可以分化成为外胚层组织，并进一步分化为神经元和神经胶质细胞。这些细胞已用于中枢神经系统疾病细胞替代治疗和治疗药物研发。

Objective: Identify the human embryonic germ cells by observing the expression of its specific surface markers SSEA-1、SSEA-3. Methods: Human embryonic genital ridges, dorsal mesenteries(5-9 weeks post-fertilization) were cultured without any cytokines, while the fibroblasts derived from the isogenous embryo were used as the feeder layer.

目的：组织块培养法培养人胚胎生殖干细胞，通过检测细胞所表达的阶段特异性胚胎抗原1 和3(stage-specific embryonic antigen-1、3 ，SSEA-1、SSEA-3)，对未分化的人EG 细胞进行特异性的生物学鉴定。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失