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Results Agarose gel electrophoresis proved that the length of PCR product was about 7 000 bp. SDS-PAGE showed that the target protein was highly expressed. Compared with those of control, the anthranilate synthase and tryptophan synthase activities of expressed product increased by 2.7 and 3.2 folds respectively.

结果 凝胶电泳可见代PCR扩增产物大小约为7000bp，SDS-PAGE鉴定目的蛋白分别得到了表达，邻氨基苯甲酸合成酶和色氨酸合成酶的活性分别比对照提高了2.7和3.2倍。

EST electrophoresis shows EST is an important antidotal enzyme system and reacts strong to environment. EST changes greatly during the whole treating period and can be viewed as pathological index of toxicity.

同工酶电泳分析表明，EST是动物体内一种重要的解毒酶系统，且是钉螺对外界刺激反应最强烈的同工酶之一，EST在整个提取物作用阶段变化明显，可作为钉螺中毒的病理指标。

Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠，融合筛选抗DR5杂交瘤细胞，制备抗DR5单抗——mDRA6；流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率；荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化；MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响；琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

Comparing the whitmania pigra Whitman with Hirudo nipponica in antithrombin activity, in the constitute of amino acid and in fingerprintings of electrophoresis.

对比了蚂蟥和水蛭的干品药材的抗凝血活性、氨基酸组成和二者蛋白质的电泳指纹图谱。

A capillary electrophoresis-mass spectrometry analytical method for apomorphine and sildenafil in medicines for Erectile Dysfunction was developed.

建立了毛细管电泳－电喷雾电离质谱联用法同时测定性保健品中脱水吗啡和西地那非的分析方法。

We use proteomic approach:two-dimensional electrophoresis and mass spectrometry to detect the proteins expression changes modulated by areca nut extracttreatment and attempted to link the correlation between OSCC and areca use.

本研究使用蛋白体学研究方法――二维电泳搭配质谱仪分析法，探讨口腔细胞受槟榔萃取物影响之蛋白质表现的改变，期望藉由高产能的策略探讨槟榔与口腔癌间的相关性。

Observations on the localization of AFP in rat liver tissue in correlation to serum AFP dynamics during 3'-MeDAB carcinogenesis were carried out by the immunoenzyme technique and the radio-rocket-electrophoresis autography.

本工作采用放射火箭电泳自显术和免疫酶标记定位技术，对大鼠3'-MeDAB诱癌过程血清和肝组织甲胎蛋白的动态变化和定位情况进行了观察。

The DNA replication is usually assayed by denatured gel electrophoresis and autoradiography, which are complex, time-consuming, and incapable of acquiring the dynamic data in real time.

目前DNA复制的研究，一般采用放射性标记与凝胶电泳相结合的方法，操作复杂，不利于方便快捷地捕获生命活动过程中的重要信息。

In vitro cleavage reaction was performed at 37℃ for 1h under the presence of Mg++. The cleavage product was analyzed by polyacrilamide gel electrophoresis. After autoradiography, the cleavage rate was counted by image analysis.

体外切割作用在Mg++的存在下，37℃反应1小时，进行聚丙烯酰胺凝胶电泳，放射自显影后经图像分析计算出切割率。

After repeating 5 times, the result shows that the genome DNA can be extracted from the fresh specimens by the methods ,but fail to be extracted from the preserved specimens with the same methods through electrophoresis.

经 5次重复实验，结果表明，用两种方法均能从新鲜标本中获得较完整的DNA ，且DNA得率较高；而对于无水乙醇保存的标本，提取出的DNA用琼脂糖凝胶电泳检测不出DNA条带，说明标本在保存过程中DNA可能发生了变化，致使提取出的DNA量太少或未分离出DNA 。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。