查询词典 electrophoresis

The separation behavior of o hydroxybenzoic acid, m hydroxybenzoic acid and p hydroxybenzoic acid in high performance capillary zone electrophoresis was investigated using cetyltrimethylammonium bromide as reversed reagent for electroosmotic flow The effects of pH of buffer solution and volume fraction of methanol on the separation, peak shape and elution order were studie

采用十六烷基三甲基溴化铵为电渗流改性剂，考察了邻羟基苯甲酸、间羟基苯甲酸和对羟基苯甲酸在高效毛细管区带电泳模式下的分离行为，研究了缓冲液的pH和添加剂甲醇的用量对这 3种同分异构体的分离、峰形和出峰顺序的影响，并据此优化了这 3种异构体的分离条件。

Capillary electrophoresis frontal analysis and affinity CE methods were also developed to determine the unbound drug concentration and to investigate the protein-binding of clozapine and imipramine.

本研究还建立了CEFA和亲和毛细管电泳法测定游离药物浓度，研究了氯氮平和丙咪嗪等与蛋白的结合作用。

The results showed the appropriate concentration of taxol (10 μM) or taxotere (35 μM) activate the influxes of H+, ROS accumulation, PAL activity and biosynthesis of taxol, as well as apoptosis, however, the high concentration taxol or taxotere (140 μM) did not show above results. Ten plots of protein were only observed in taxol-induced cells and there were differences at expression level of some plots between control and taxol-induced cells using two dimension electrophoresis, which reflect those differential protein were highly relevant to signal moleculars of cell defense responses, suggesting cell apoptosis and taxol biosynthesis might be parallel or two events in the same pathway of defense responses.

采用二维凝胶电泳技术，分析了taxol诱导细胞产生早期防御响应的蛋白表达差异，发现taxol诱导了10个新蛋白点的表达，其中一些新蛋白点的分子量与细胞信号分子G-蛋白、Ca2+依赖蛋白激酶的分子量接近。

Methods: Male Sprague-Dawley rats were subjected to lateral fluid percussion brain injury of moderate severity. bax and bcl-xL mRNA and protein expression was detected by RT-PCR an d immunohistochemistry. In addition to morphological evidence of apoptosis, TUNE L histochemistry was used to identify DNA fragmentation in situ under both l ight and electron microscope, whereas characteristic internucleosomal DN A fragm entation of apoptosis was demonstrated by DNA gel electrophoresis.

在液压脑损伤模型中，应用逆转录聚合酶链反应、免疫组化分别检测大鼠脑创伤后不同时程bax和bcl-xL表达；采用凋亡原位末端标记、电镜超微结构、DNA凝胶电泳观察脑创伤后细胞凋亡的形态和生化特征。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

T sensitive to organic solution.CONCLUSION:The SOD purified from shark liver was found to be homogenous as shown by the pattern of polyacrylamide electrophoresis,specific activity,molecular weight and ultraviolet absorption spectrum.

根据聚丙烯酰胺凝胶电泳、比活、相对分子量及紫外吸收光谱等性质的测定，证实从鲨鱼肝中提纯的SOD是均一的。

In this expeviment, SDS-polyacrylamide gel electrophoresis was used to analysis the effects of cold stimulation on the types of blood protein of two species of Loaches.

采用SDS-聚丙稀酰胺凝胶电泳技术，探讨冷刺激对泥鳅血液蛋白质的影响。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明，除了能复性成天然态的溶菌酶分子外，还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体；而它们的还原电泳和在SDS存在时的复性结果表明，这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成，而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

Six isozymes, extracted from three tissues of Hemibarbus maculates Bleeker respectively,were analysed with vertical slab polyacrylamide gel electrophoresis.

作者：顾若波，闻海波，华丹，徐钢春(中国水产科学研究院淡水渔业研究中心，农业部水生动物遗传育种和养殖生物学重点开放实验室，江苏无锡 214081)点击数：217

A method for determination of Malachite green in fishes was established by high performance capillary electrophoresis using self-constructed conductivity detection system.

建立了鱼类中孔雀石绿残留量的高效毛细管电泳－电导法。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐