查询词典 electrophoresis

To examine the hypothesis, we test whether maize beta-amylase can serve as a lipase inhibitor.In order to conduct the interaction assay of beta-amylase and lipase, we adopted a preparative electrophoresis method with a Prep Cell to purify beta-amylase proteins from germinating maize endosperms and soybean cotyledons. We pre-mixed tested proteins with lipid substrate prior to the addition of lipase preparation and assayed the lipid hydrolytic activity.

本实验利用Prep Cell纯化玉米萌芽谷粒和大豆子叶中的beta-淀粉酶，分别将两种不同物种的beta-淀粉酶与胰脂解酶预先反应后，再加入脂解酶反应液中观察活性的变化，结果发现脂解酶的活性未如预期，没有抑制的效果产生，将甘薯beta-淀粉酶以同样方法与胰脂解酶作用，结果同上述两种beta淀粉酶依旧没有抑制的现象。

Sitafloxacin epimers were separated by capillary zone electrophoresis using γ-cyclodextrin and D-phenylalanine as chiral selector.

以γ-环糊精和D-苯丙氨酸为手性选择剂，采用毛细管区带电泳成功地分离了新型抗菌素西他沙星差向异构体。

Thymocytes were also pretreated with or without ERKs, JNKs and p38 kinase inhibitors prior to UVB irradiation and DNA fragmentation was assessed by agarose gel electrophoresis. The activation of ERKs, JNKs, p38 kinase were investigated by western-blot.

透射电镜观察细胞超微结构的改变；琼脂糖凝胶电泳观察各组不加入或分别加入ERK抑制剂、JNK抑制剂和P38抑制剂后的DNA ladder；western-blot检测磷酸化ERK、磷酸化JNK、和磷酸化P38的活性。

The effect on diversity of eubacteria in submerged paddy soil with phenanthrene was also studied using the denaturing gradient gel electrophoresis technique.

在传统微生物污染生态研究基础上，运用PCR-DGGE方法，对菲污染条件下稻田土壤真细菌种群的基因多样性进行了初步研究。

The electrophoretograms of isozymes from five different tissues （foot, mantle, adductor muscle, gill, liver） of Mercenaria mercenaria Linnaeus were analyzed, by vertical polyactylamide gel electrophoresis.

采用垂直板聚丙烯酰胺凝胶电泳技术，对硬壳蛤5种组织（足、外套膜、闭壳肌、鳃和肝）的酯酶、苹果酸脱氢酶和乳酸脱氢酶同工酶进行了比较研究，并对酶谱表型及位点表达进行了分析。

Extracted solutions of different parts foregut , midgut and hindgut of alimentary canal of bee were isolated by polyacrylamide gel electrophoresis .

采用聚丙烯酰胺凝胶电泳分离意蜂和中蜂消化道各部分肠液

The enzymes were furth er separated and purified into 12 components after DEAE-32 chromatography and p reparative electrophoresis.

经DEAE-纤维素离子交换和制备电泳进一步分离纯化，得到12个单一组分。

After 3d of gastric gavages,peripheral blood was taken from experimental mice to extract the lym phocytes.Then single cell gel electrophoresis test Was used to detect the inhibition of DNA.DNA damage in mice.

材料与方法：固相萃取水中有机物，用不同浓度维生素C水溶液(50、100、200 ms／ks)与有机物以不同方式处理(同时灌胃、维生素C预处理、维生素C后处理)，分别给小鼠灌胃3 d后，取外周血淋巴细胞进行单细胞凝胶电泳实验，检测细胞DNA的损伤情况。

Speculating on the figure of two dimensional electrophoresis of rice glutelins there are at least 13 glutelin genes, only six of which, GluA-1, GluA-2, GluA-3, GluB-1, GluB-2 and a cDNA clone,λ RG21 cDNA, have been sequenced.

从水稻谷蛋白的2-D电泳可推测，编码水稻谷蛋白的基因至少有13个，目前尚只有6个水稻谷蛋白基因被分离测序，它们分别是GluA-1，GluA-2，GluA-3，GluB-1，GluB-2和一cDNA克隆（λRG21 cDNA），而且，这些基因都是通过实验筛选大量的水稻基因组文库或cDNA文库获得的，其过程成本高，工作量大，实验周期长。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

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集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐