查询词典 electrophoresis

Methods A method combining pulsed field gel electrophoresis with low-frequency-cleavage restriction endonucleases was established to analysis DNA of Enterobacter cloacae.

方法利用脉冲场凝胶电泳结合低频限制性内切酶技术对阴沟肠杆菌染色体DNA进行分析。

It will provide the guidance for produce the universal cathode electrophoresis colorants to a certain extent.

这对生产通用型阴极电泳色浆有一定的指导意义。

Medium Choi, conches, Shide, Longqiao Materials such as hundreds of brands and my company has three subsidiary factories and companies in the electrophoresis, spraying, insulation type wood, wood grain transfer printing and the production of doors and windows installed a full set of advanced equipment.

中财，海螺，实德，龙桥等上百个品牌材料并且我公司有三个下属工厂和公司分别在电泳、喷涂、隔热型材、木纹转印和门窗生产安装的全套先进设备。

Through comparing exon sequences at both ends of introns of CHD genes of jungle fowls and cygnets, exons on two ends thereof are respectively designed with an upstream primer and a downstream primer: Psf: 5'CAAGGATGAGAAACTGTGCAA3'; and Psr: 5'GAATATCTTCTGCTCCTACTG3'; and the Psf/Psr primers are used for amplifying the CHD genes on Z and W chromosomes of meat pigeon feather pulp genome DNA, and finally the sex is identified according to the band type through 10 percent polyacrylamide gel electrophoresis and silver staining, wherein, female shows two bands and male shows one band.

通过原鸡和小天鹅CHD基因内含子两端的外显子序列比对，在两端外显子上分别设计上下游引物：Psf：5'CAAGGATGAGAAACTGTGCAA3'；Psr：5'GAATATCTTCTGCTCCTACTG3'；再以Psf/Psr引物扩增肉鸽羽髓基因组DNA的Z和W染色体上的CHD基因，最后经过10％聚丙烯酰胺凝胶电泳、银染，根据带型判断性别，其中雌性为两条带，雄性为一条带。

DNA efficiency and quality can be analysed by agarose gel electrophoresis and double enzymatic digestions.

结果 C、D法提纯的质粒DNA的含量、纯度和收获率均较A、B高，上述4 方法提纯的质粒DNA都能进行酶切反应。

Dna efficiency and quality can be analysed by agarose gel electrophoresis and double enzymatic digestions.

结果 c、d法提纯的质粒dna的含量、纯度和收获率均较a、b高，上述4种方法提纯的质粒dna都能进行酶切反应。

Screening and identification: the resulting plasmid was extracted by alkali division, identified by restriction enzyme digestions and PCR, observed by 1% agarose gels electrophoresis.

为了提高了目的片段和载体之间的连接效率以及目的片段的拷贝数，本实验所选用的载体是pMD18-T Vector，它是由pUC18用EcoRⅤ切割后在两个3'末端加上单个的碱基'T'而制得的线状分子。

Methods: Electrets Teflon PTFE,±300 V,±1 000 V were used to treat 3T3 cells for 24, 48 and 72 h. Then the influences of electrets on cell cycle and surface charge of 3T3 cells were studied by flow cytometry and electrophoresis, respectively.

选用±300 V和±1 000 V驻极体分别作用成纤维细胞24、48和72 h，用流式细胞术和细胞电泳技术测定成纤维细胞的生长周期和细胞表面电荷。

The -300V and -500V electrets were applied on 3T3 cells for 24 and 48h, respectively, then the surface charge density on 3T3 cells and the intracellular free Ca2+ concentration and the number of apoptosis were measured by means of cell electrophoresis and flow cytometry method, respectively.

利用Fluo—3/AM Ca2+指示剂，采用流式细胞术测定3T3细胞内钙离子浓度的变化和3T3细胞的凋亡量，探讨驻极体诱导3T3细胞凋亡与细胞表面电荷密度和游离钙浓度变化之间的关系。

Furthermore, from sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis results, it was found that the protein concentration difference was reduced obviously in the eluant of direct sample loading, and most high abundance proteins were identified in the eluant of NaCl.

此外，凝胶电泳的分析结果显示，在上样流出液中蛋白质的浓度差异明显变小，大量的高丰度蛋白质存在于NaCl洗脱液中。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐