查询词典 electrophoresis

Using uncontinued polycrylamide gel electrophoresis, comparative studies of isozymes between the four geographical populations of Tomicus piniperda L.

采用不连续聚丙烯酰胺凝胶电泳技术研究了纵坑切梢小蠹Tomicus piniperda L。

In the present study, efficient identification methods for the main varieties of cowpea (Vigna unguiculata W.) and cauliflower (Brassica oleracea L.) in south of China, especially in zhejiang province, were investigated by using seven cowpea varieties (variety 1, Zhijiangtechang80;variety2, Zhijiangtechang90;variety3, Qiujiang512;variety4, Zhijiang228-2;variety5, Huajiang24;variety6, Yangjiang40;and variety7, Xingaochan8) and five cauliflower varieties (variety A, Longfenteda50tian;variety B, Yidaijinguang80tian;variety C, RuixuetedalOOtian;variety D, Qiuwang80tian;and variety E, Tezao50tian). The application of seed shape, seedling shape, rapid chemical identification and electrophoresis was performed, the main results were as follows.

为开展南方特别是浙江省主要蔬菜作物种子高效快速鉴定技术研究，本试验以七个豇豆品种品种1(之豇特长80，品种2(之豇特长90)，品种3(秋豇512)，品种4(之豇228—2)，品种5(华豇24号)，品种6(扬豇40号)，品种7和五个花椰菜品种品种A(龙峰特大50天，品种B(一代金光80天)，品种C(瑞雪特大100天)，品种D(秋王80天)，品种E(特早50天)为材料，从种子形态、幼苗形态、快速化学和电泳鉴定等方面对豇豆和花椰菜品种鉴定进行了研究，特别是对乳酸—聚丙烯酰胺凝胶电泳在这两种蔬菜品种鉴定上的可行性进行了研究。

A simple, fast, and reliable method based on capillary electrophoresis with electrochemical detection was developed for the separation and determination of rutin, ferulic acid, vanillic acid, chlorogenic acid, quercetin and caffeic acid in Radix Astragali and its medicinal preparations.

采用毛细管电泳－电化学检测法对中药黄芪的主要活性成分芦丁、阿魏酸、香草酸、绿原酸、槲皮素和咖啡酸进行了分离和测定。

The major bioactive components in a Chinese herb named Polygonum perfoliatum L. including ferulic acid, vanillic acid, quercetin, caffeic acid and protocatechuic acid were simultaneously determined by capillary electrophoresis with electrochemical detection in this paper.

采用毛细管电泳-电化学检测法同时分离测定了中药杠板归中的阿魏酸、香草酸、槲皮素、咖啡酸、原儿茶酸等主要生物活性成分的含量。

The iso-electric point of was 4.8-5.2. The amino acid analysis indicated that the recombinant protein is non- N-glycosylated.The ability of 0.5 ug/m rhsTRAIL to induce apoptosis of liver cancer cells SMMC7721 and BEL 7402 could be visioned morphologically. This was further verified by Electrical microscope observision of induced SMMC7721 cells and agarose gel electrophoresis of apoptosis DNA. MTT test results also showed that 0.01 u g/ml purified rhsTRAIL has strong effect to induce apoptosis of SMMC7721 cell.

形态学上观察了rhsTRAIL对肝癌细胞SMMC7721和BEL7402的诱导凋亡活性，发现rhsTRAIL在0.5μg/ml时显示了很好的诱导凋亡活性，并通过电镜观察rhsTRAIL诱导后的SMMC7721细胞，证实了凋亡；同时提取凋亡细胞DNA，进行琼脂糖电泳分析，在分子水平证明凋亡；最后利用SMMC7721细胞分组加入纯化后的rhsTRAIL蛋白，结果显示纯化蛋白含量在0.01μg/ml时细胞就呈现了诱导凋亡活性。

Liver ferritin of Sphyma zygaena with small-scale preparation was done by electrophoresis approach.

小批量制备了电泳纯鲨鱼肝铁蛋白(Liver ferritin of Sphyma zygaena ， SZLF)。

In these fields, much attention has been given to development of apparatus of microfluid chip and capillary electrophoresis, injection technique, coating technique and separation matrix.

当前，在单个细胞及单分子水平上研究生命物质的含量、组成、代谢及其相互作用是生命科学的一个研究热点。

In this thesis, the microfluid chip electrophoresis apparatus with laser induced fluorescence and charge-coupled device was set up in our lab and two model sample, EOS and fluorescein, were separated in a few seconds.

而建立高灵敏度、高速高效的分析方法以研究生物微环境中的超痕量物质是研究单个细胞或生物囊泡复杂的生命物质的含量、组成的关键。

Combined with high sensitive detective method, such as laser-induced fluorescence, electrochemistry, chemiluminescence and mass spectrometry, microfluid chip and capillary electrophoresis is becoming one of important and useful tool in post-genome era including proteomics as the results of theirs high performance, high speed and high throughput.

在生命科学的研究中，以毛细管电泳、微流控芯片毛细管电泳为代表的高速、高效、高通量分离分析技术与以质谱、激光诱导荧光为代表的高灵敏度、高选择性检测技术的联用，已成为后基因组时代的主流技术，是最重要的研究工具之一。

Based on numerical computation, glass microfiuidic chips are fabricated, and experiments using microfluid fluorescence visualization technology are conducted to study sample electrophoresis transport characteristics in microchannel turns, with the aim to verify results of numerical computation.

在数值计算的基础上，制作了玻璃微流控电泳芯片，采用荧光可视化微流体实验技术，在微流控动态观测平台上实验研究了弯曲微通道中的样品电泳输运特性，验证了数值计算结果的正确性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐