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We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

The electrophoresis patterns of EST and SOD had a tissue-specific distribution. The Sod should consist of two loci, which code dimeric s-SOD and tetrameric m-SOD respectively.

结果表明，二色裂江珧组织内的EST和SOD存在不同程度的组织特异性，EST共检测出15条酶带，SOD检测出8条酶带，且两种同工酶在消化盲囊中活性最高。

Methods: Eleven B-CLL patients were studied. Leukemic lymphocytes with (n=8) or without (n=3) P2z receptors were exposed in vitro to ATP, benzoylbenzoic-ATP, 2-methylthio-ATP(2MeSATP), adenosine-5′-［γ-thio］ triphosphate, and other nucleosides for 8h. Apoptosis was detected by electron microscopy, agarose gel electrophoresis, and quantitative TdT assay. Results:Apoptosis was detected only in leukemic lymphocytes with P2z receptors. By using the quantitative assay, ATP-inducing DNA strand breaks were found to occur specifically for BzATP, ATP and 2MeSATP, but not for ATP-γS and other nucleosides.

将表达P2z受体［P2z］与不含P2z受体［P2z］的两组CLL细胞分别同1.0mmol/L三磷酸腺苷体外培养8小时，以电镜、DNA凝胶电泳和定量DNA 3′端TdT法检测细胞凋亡；并对ATP、苯甲酰苯甲酸ATP、2-甲基硫ATP(2MeSATP)、γ-硫代ATP及其它核苷的诱导效应和氧化型ATP、1-［N，O-二(5-异喹啉碘酰基)N-甲基-L-酪氨酰］-4-苯哌嗪KN-62）的抑制效应做定量研究。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

Methods One hundred thirty PCOS women and one hundred seventy five normal women as controls were enrolled in this study. The genotypes were screened by polymerase chain reaction-On/off switch and the product was isolated by electrophoresis on a 2.5% agarose gel containing ethidium bromide and visualized using an ultraviolet transilluminator.

应用On/off Switch-聚合酶链反应及琼脂糖凝胶电泳分离技术分析130例多囊卵巢综合征患者及175例对照组妇女TNF-α基因rs3179060C/A多态，比较rs3179060C/A两组等位基因及基因型与PCOS及其高雄激素之间的关系。

Methods The genomic DNA extracted from the 2 trematodes was amplified with random amplified polymorphic DNA and then was carried on agarose gel electrophoresis.

应用随机扩增多态性DNA（Random amplified polymorphic DNA，RAPD）技术对斯氏肺吸虫和华支睾吸虫的基因组多态DNA进行扩增，扩增产物经琼脂糖凝胶电泳后分析结果。

CD was added into running buffer in above electrophoresis system, and the separation efficiency of polycarboxylic acids was improved further by the supramolecular interaction. Based on that, the method for separation and determination of several di- and tricarboxylic acids, including oxalic, malic, tartaric and citric acids, found in fruit juices was developed, which supplied a fast, convenient and sensitive method for the food analysis, the quality control of foods and various drinks.

果汁中多元有机酸的毛细管区带电泳/Cu电极安培检测在上述分析体系的电泳介质中添加β-CD，利用CPB与β-CD的协同超分子作用，进一步提高了多元有机酸的分离效率，成功实现了水果中四种常见多元有机酸草酸、柠檬酸、苹果酸、酒石酸的基线分离，建立了几种果汁中上述有机酸毛细管区带电泳/Cu电极安培检测定量测定方法，为食品分析、食品饮料质量控制以至辨别酒类商品真伪等提供了一种方便、快速、灵敏度高的分析方法。

In this sense, IDA plays dual effects, tridentate chelating to Cu(2) and bridgebetween two Cu(1) andCu(2).3 Metal complexes were selected as the appropriate for the study of cleavage plasmid pBR322DNA by gel electrophoresis technique. The results showed Ni and Mn complexes could cleave effect- ively DNA in the presence of H2O2 at physiological pH and temperature, whereas individual Zn complex could cleave effectively DNA.

通过电泳实验研究了一系列金属配合物与pBR322DNA的作用，发现在Tris-HCl缓冲溶液中，生理条件下，镍、锰配合物在共反应物H_2O_2存在下能够很好的断裂DNA，而Zn配合物单独作用就能够使DNA由ccc型转化为oc和linear型。

The microheterogeneity and tryptic fragments of glycoprotein recombinant human erythropoietin were analyzed by high performance capillary electrophoresis.

用高效毛细管电泳测定了重组人促红细胞生成素的微多相性、肽图，同时采用毛细管电泳质谱联用技术鉴定了部分非糖基化胰酶消解片段和O－126糖基化肽的结构。

Methods SOS/umu assay and the ethidium bromide flurescence assay and the single cell gel electrophoresis assay were applied respectively to detect the DNA damage and DNA cross - links and DNA single strand breaks after exposure to the particle extracts of air pollutants in TSCDG.

方法应用SOS/umu试验，大鼠肺Ⅱ型细胞的DNA交联试验和单细胞凝胶电泳试验，检测危险品货运站空气污染物对DNA的损伤情况。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。