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On the 12〓 day, classical DNA ladder appeared after electrophoresis. Fine structural observation showed that the senescence process in this system is a highly ordered programmed cell death process, and its function was speculated as nutrition remobilization. All these suggested that this system is a plant cell apoptotic system.

从第8天开始有些细胞核开始出现TUNEL阳性反应，在处理12天时，总DNA电泳中出现了典型的DNA ladder，超微结构观察发现其衰老过程是一个高度有序的程序化死亡过程，推测功能上是一个营养物质再利用的过程，因而这一体系是一个细胞凋亡体系。

Sample preparations such as prevention of protein degradation, removement of cell element, concentration, degreasement, desaltation, and albumin depletion were taken to meet the demands of two-dimensional polyacrylamide gel electrophoresis.

用防止腹水蛋白质降解、去细胞成分、浓缩、去脂、脱盐及去干扰大的白蛋白等腹水的前期处理以满足双向凝胶电泳的条件。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

Using the MTT assay, agarose gel electrophoresis, transmission electron microscope, TUNEL method and immunohistochemistry (PCNA, bcl-2),it was studied in this experiment that the inducing APO, anti-proliferation and affection of expression of bcl-2 gene before and afterAPO of the Rubia Cordifolia L with the 5-fluorouracil(5-fu) for MGC-803 cells of gastric carcinoma.

本实验通过MTT法、琼脂糖凝胶电泳法、透射电镜观察法、TUNEL法、免疫细胞化学法（PCNA、bcl-2），研究了茜草提取物联用5-氟尿嘧啶（5-fluorouracil，5-Fu）对胃癌MGC-803细胞株的诱导凋亡、抗增殖作用及凋亡前后bcl-2基因表达的影响。

objectiveto establish a peroxidase isoenzyme method for identification of rugosa rose varieties in shandong province.methodsthe polyacrylamide gel electrophoresis method was applied.

目的建立山东不同品种玫瑰花药材的过氧化物酶同工酶鉴别方法。方法聚丙烯酰胺凝胶电泳法。

ObjectiveTo establish a Peroxidase isoenzyme method for identification of rugosa rose varieties in Shandong province.MethodsThe polyacrylamide gel electrophoresis method was applied.

山东作为玫瑰花的道地产区，汇集了当今玫瑰的主要品种。2005版《中国药典》对玫瑰花的质量标准收载的较少，并且在不少地区发现其混淆品现象。

The biological characteristics and toxicity of Russula subnigricans Hongo was studied for the first time from ecology and morphologic characteristics and histology, the orthogonal experiment of the optimum culture condition, the analysis of components, apoptosis of the cells from little white rat liver and kidney induced by extract of Russula Subnigricans Hongo,to the histopathologic changes observation of little white rat liver and kidney through ecological observation, light microscopy and scanning electron microscopy, reversed-phase high performance liquid chromatography, agarose gel electrophoresis, transmission electron micioscopy.

利用菌种分离技术、光镜技术、电镜技术、高效液相色谱技术、毒理实验技术、电泳方法等对亚稀褶黑菇的生物学特性和毒性机理进行了研究，主要包括以下内容：亚稀褶黑菇的生态学和组织学研究、菌种分离培养、扫描电镜观察、成分分析、粗毒液诱导小自鼠肝肾细胞凋亡，小白鼠中毒后肝肾细胞透射电镜观察，研究结果如下： 1。

This research concerns about the isozymes analysis of parasitic sarcocystis hirsuta from water buffalo and cattle by means of isoelectric focusing electrophoresis to explore the host specificity of sarcocystis species.

采用聚丙烯酰胺垂直平板梯度凝胶电泳的方法，对寄生于黄牛和水牛的毛状肉孢子虫进行同工酶比较研究，探讨肉孢子虫的宿主特异性。

The gel electrophoresis demonstrated the Model Group appeared the scalariform bands, but the scalariform bands of the treatment Group compared to the Model Group was't obvious.

凝胶电泳显示模型组出现梯状条带，而治疗组较模型组梯状条带不明显。

Objective To study H2O2-induced single strand breaks formation in DNA and the reparation In NIH3T3 mouse fibroblasts by single cell gel electrophoresis technique and to introduce SCOB.

目的 观察小鼠成纤维细胞由过氧化氢(H2O2)引起的DNA损伤及其修复，并详细介绍单细胞凝胶电泳技术。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。