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It shows that coexpression of secE/Y gene can increase a new band of about 25kD in the periplasm, but further electrophoresis analysis and Western blot prove that this band may not be the assembled TGF β1 dimer.

发现共表达secE／Y基因可使周质中新增25KD的蛋白带。

Benzyl isothiocyanate and phenethyl isothiocyanate induced apoptosis of DU-145 cells which also were confirmed by DAPI staining and DNA gel electrophoresis.

除此之外，苯甲基异硫氰酸酯和苯乙基异硫氰酸酯也会造成人类前列腺癌细胞株DU-145粒线体膜电位的改变，活性氧化物质和钙离子的释放以及造成DNA的损伤。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Hydrazine compounds including hydrazine, phenylhydrazine, semicarbazide and isoniazid were separated and determined by capillary electrophoresis with a poly modified microdisk carbon fiber electrode.

肼类化合物的毛细管区带电泳/聚谷氨酸修饰碳纤维微盘阵列电极安培检测以电聚合方法制备了聚谷氨酸修饰碳纤维微盘阵列电极，研究了肼、苯肼、氨基脲及异烟肼等肼类化合物在该电极上的电催化氧化。

With the vertical flat electrophoresis of polyacrylamide gel, 12 Pholiota adipose strains from different regions were compared and analyzed in esterase isozyme character and the genetic differences among strains were analyzed with the cluster analysis software.

方法］利用垂直板聚丙烯酰胺凝胶电泳，对来源于不同地区的12个黄伞菌株进行酯酶同工酶比较和分析，并通过聚类分析软件对各菌株间的遗传差异性进行分析。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

Results 5-FU inhibits growth of Lovo cells; it is found that within a limited dosage and period of time, there has been an obvious increase in apoptosis cells under the photomechanical scope; the analysis of Lovo cells by FCM showed that after the induction of 5-FU, the percentage of apoptosis cells has increased; and FCM also displays dosage and time effects. Electrophoresis was obviously in a stripe shape of trapeziform.

结果 5－FU能抑制 Lovo细胞生长，在一定剂量和时间范围内，通过光镜观察，见凋亡细胞明显增多，经5－FU诱导后，用FCM 分析 Lovo细胞，发现凋亡百分率增加并显示剂量和时间效应。

This article briefly reviews the electrophoresis and chromatography of polysaccharose.

鉴于以上情况，本文对多糖的电泳和层析方面进行了综述。

The results of research showed that compound ferrous flocculant has simple preparation and not any second pollution matter is produced to the environment, it has big and stout floes formation and fast speed sedimentation while dealing with waste water, it is positively charged according to the electrophoresis, it belongs to cationic flocculant, can gives better coagulation results in turbidity removal, color removal, the maximum removal rate of turbidity and color are 95.4% and more than 81.7%, respectively. There are interactions between polysilicic acid and hydrolyzed ferrous species, the compound ferrous with appropriate Si/Fe not only but also can lower the water treatment cost. The compound ferrous removes colloid particles in water by charge oxidation-reduction, neutralization and furl mechanism.

研究结果表明：复合亚铁制备工艺简便，无二次污染；处理水样时产生的矾花大，密实、沉降速度快；复合亚铁带电性为正，为阳离子型絮凝剂；复合亚铁具有较好的除浊、脱色能力，除浊率及脱色率最高分别可达95.4％及81.7％；在复合亚铁中，聚硅酸与亚铁的水解产物间存在着相互作用，合适的硅铁摩尔比有利于提高混凝效果，而且还可以降低投药成本；复合亚铁是通过氧化还原、吸附电中和及卷扫三种作用机理起混凝作用的。

Proteins were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto polyvinylidene fluoride membranes.

用12 ％十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离蛋白质再转移到PVDF膜。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。