查询词典 electrophoresis

A preliminary study on substrate specificity of Lumbricus Bimastus nucleaseTo measure degrade rate of DNase E to several different substrate in optimal reaction condition to demonstrate the substrate selectivity of DNase E. Agarose gel electrophoresis was used to investigate the products of DNase E processing double strands cyclic DNA to investigate the action of DNase E.

双胸蚓组织核酸酶底物特异性的研究测定DNase E在最适反应条件下对不同底物的降解速率，证明DNase E降解底物的选择性；应用琼脂糖凝胶电泳对DNase E水解双链环状DNA后的产物进行鉴定，阐明DNase E的水解方式。

Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析，8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达，表达蛋白分子量为140～150kDa，抗原cDNA基因经限制性内切酶EcoRI酶解后，琼脂糖凝胶电泳显示其大小为700～900bp，PCR能扩增出特异性条带。

Methods Blood samples of 300 unrelated individuals from Chengdu, Bangkok and Maint were taken and analyzed with single PCR, polyacrylamide gel electrophoresis and silver staining. Results In the three loci, 9, 6, and 8 alleles and 32, 14, and 22 genotypes were found respectively.

采用PCR、非变性聚丙烯酰胺凝胶电泳及银染技术分析中国成都地区汉族、泰国曼谷地区人群以及德国Maint地区人群中三个基因座的遗传多态性，获得三个基因座的群体遗传学数据。

By the analysis of SDS-PAGE electrophoresis, CXJZ95-198 secrets exocellular proteins bands in media of glucose, mannose ,xylan, mannosan and pectin respective were 32 bands, 33 bands,35 bands, 36 bands and 21 bands respectively .

在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中的发酵液在SDS-PAGE电泳中分别分泌出32条，33条，35条，36条和21条蛋白带；多数可比较的蛋白质电泳谱带的颜色深浅呈魔芋粉＞甘露糖＞木聚糖＞葡萄糖＞果胶的趋势。

The thesis studied the total exocellular proteins, reductant sugar and the pectinase enzyme activity of CXJZ95-198 strain in glucose, mannose, xylan, mannosan and pectin culture media.Compared enzyme activity of pectinase, xylanose and β-mannanose. The secreted proteins in ferment fluid from different culture media were analysed by SDS-PAGE electrophoresis.

本论文对CXJZ95-198菌株在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中胞外酶蛋白质总量、还原糖及果胶酶活性的影响进行了研究；对果胶酶、木聚糖酶及β-甘露聚糖酶在甘露糖培养基中的酶活进行了比较；对CXJZ95-198菌株在不同碳源培养基中的发酵液在SDS-PAGE电泳中分泌出的蛋白带作了比较；对CXJZ95-198菌株所产生的胞外酶系中果胶酶进行了分离纯化。

Morphologically, the macrophages treated with NO and O-LDL presented shrinkage, nucleus fragmentation, and cytoplasm condensation; DNA ladder was visualized by agarose gel electrophoresis; Characteristic apoptosis peak of macrophages was showed by flow cytometry; Ultrastructure, The chromatin condensed and marginated to form dense chromatin fragmentation; The cytoplasm concentrated and vacuolized; The lysosomes and mitochondria of apoptosis macrophages increased clearly.

电镜观察超微结构变化：染色质固缩边聚，形成致密、均质的染色质块，胞质浓缩、电子密度升高并空泡化；凋亡巨噬细胞溶酶体、线粒体增多；细胞突起缩短减少，但巨噬细胞特有的板状伪足不消失。

Using polyacrylamide gel electrophoresis, the peroxidase isoenzyme of three different resistant varieties of apple seedlings inoculated with Marssonina coronaria was studied.

应用聚丙烯酰胺凝胶电泳，测定抗性不同的3个苹果品种过氧化物酶同工酶在接种褐斑病菌后的变化规律。

The biochemical genetics in 5 populations of Meretrix meretrix from northern China were analyzed by polyacrylamide gradient gel vertical electrophoresis.

采用聚丙烯酰胺凝胶电泳技术，对文蛤5个北方种群进行了生化遗传分析。

Using two-dimensional gel electrophoresis, the protein of whole mantle of patterned and non-patterned Meretrix meretrix , from Yueqing, Zhejiang, East China, was studied in mass spectrometry.

中文摘要：采用双向电泳技术，对浙江乐清无花纹和有花纹文蛤外套膜的全蛋白进行了研究，并利用质谱和软件对差异蛋白进行了分析。

Separation of the PCR products and fluorescence detection were performed by ABI prism 310 Genetic Analyzer with capillary electrophoresis. The Genescan and Genotype soft ware were used for size calling and quantification of peak areas. The formula to calculate donor chi?merism values was based on the different allelic distribution type between donor and recipient.

HSCT后转归的预警作用，采集27例清髓性外周血干细胞移植患者移植前、移植后不同时段的外周血或骨髓，DNA样本用Profiler Plus和Cofiler Plus商品化试剂盒扩增后，用ABI310遗传分析仪进行毛细管电泳，确定基因位点及峰面积，根据基因型的差异选择嵌合率计算公式。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。