查询词典 electrophoresis

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Considering the resemblance between paper chromatography and electrophoresis on distinguishing mixture of different monosaccharides or amino acids and eluting of interest component,we have a try to separate the components of some amylases which have been hydrolyzed by paper chromatography before polyacrylamide gel electrophoresis.

考虑到纸层析技术与电泳技术在分离物质(都可以对糖类混合物或者氨基酸混合物进行分离)和后期处理(都可以将含有目标成分的部分进行洗脱)电泳技术的相似处，本文在使用电泳分离出F_1-ATP合酶之前，先对水解后的多糖进行了尝试性的纸层析分离。

No US-pin plastic treatment therapy, a comprehensive alternative to traditional plastic injection of the United States (with the needle and micro-needle) is efficient, non-invasive and safe,"mesoderm into beauty therapy" is the latest technology of water use and water channel protein electrophoresis principle of development Note the infiltration hole electric technology to non-needle injection principle, the use of "point to a plane," AMD permeation technology in skin electroporation Act under the influence of pulsed current stimulation, so that pores have a physical reaction to the rapid spread in two seconds 650-800 of fine pores, forming an instant electronic delivery channels of nutrition, nutrition Note dialysis therapy immediately with electrophoresis, soft laser stimulation and ice, etc., in the most secure and fast, the skin will be required for nutritional products , 99% transmitted to the basal cells, and can be stored as long as 72 hours, the skin smooth and more compact, creating a perfect Beautiful Skin.

无针美塑治疗疗法，是全面替代传统注射美塑的高效、无创，安全的&中胚层导入美容疗法&，是运用最新技术水电泳和水通道蛋白原理研制出的电孔注渗技术，以无针注射原理，采用&点到面&超微渗透技术，其电穿孔法令肌肤在脉冲电流刺激影响下，使毛孔产生物理反应，在两秒内迅速张开650-800个微细毛孔，瞬间形成电子营养输送通道，营养注渗疗法随即展开，配合电泳、柔激光和冰封等刺激作用，在最安全和快速的情况下，将皮肤所需的营养产品， 99%输送到基底层细胞，并可储存72小时之久，令肌肤更紧致柔滑，缔造无瑕美肌。

No US-pin plastic treatment therapy, a comprehensive alternative to traditional plastic injection of the United States (with the needle and micro-needle) is efficient, non-invasive and safe,"mesoderm into beauty therapy" is the latest technology of water use and water channel protein electrophoresis principle of development Note the infiltration hole electric technology to non-needle injection principle, the use of "point to a plane," AMD permeation technology in skin electroporation Act under the influence of pulsed current stimulation, so that pores have a physical reaction to the rapid spread in two seconds 650-800 of fine pores, forming an instant electronic delivery channels of nutrition, nutrition Note dialysis therapy immediately with electrophoresis, soft laser stimulation and ice, etc., in the most secure and fast, the skin will be required for nutritional products , 99% transmitted to the basal cells, and can be stored as long as 72 hours, the skin smooth and more compact, creating a perfect Beautiful Skin.

B。电穿孔技术：直接作用于皮肤，瞬间增强皮肤组织的可渗透性。在电击的作用下，细胞的脂质二重层上形成电击孔。在电击孔形成的同时，使得原先无法被细胞吸收的亲水性分子能够穿透并进入细胞内部。电击孔一旦形成，则根据电击的长度，在数秒种至数分钟之内保持打开状态。C。电渗透技术：电渗主要作用于将要分散的物质分子，帮助其顺利穿透并进入肌肤内部，性质相同的电荷相互排斥，因此对正极的营养分子施加正电流，可将营养分子推入皮肤组织。此时，中性分子也一道穿透并进入肌肤内部。

With a long history and solid technical strength, our factory introduced advanced steel rolling equipments, and selected domestic and exported stainless steel materials. After hammering and planishing, top-grade strips and finished products of hinges are manufactured. In order to reach the quality standard of standardization and 0-defect, these years we keep introducing precision pressing equipments, automatic production equipments and related developing software to enhance the precision rate of the products and researching efficiency. To ensure better quality guarantee for the clients, we enlarge the production scales and enhance the grade of products. With the electrophoresis equipments and supporting electrophoresis paint exported from abroad, our products own the performance of anti-vibration, wearable, stainless, and anti-depigmentation.

我们建厂历史悠久，技术力量雄厚，同时引进先进轧钢设备，选用进口、国产不锈钢原材，经过锻打、精轧，制成优质带材，直至合页成品，一条龙生产，为达到标准化、零缺点的品质水准，历年来不断引进精密冲压设备与自动化生产设备及相关的产品开发软件，以提高产品精确度及生产研发效率；让客户享有更佳的品质保证，为扩大生产规模，提高产品档次，专门引进国外电泳生产设备、配套进口电泳原漆，从而使产品更具有抗震、耐磨，不生锈、不退色等特点。

Results: In cellular soluble protein electrophoresis,specific band about 75 KDa was observed in 593 strain.The differences of protein expression at 85,60,50,40KDa between 593 strain and 18 strain were observed.In extracellular soluble protein electrophoresis,specific bands at 75 KDa and 40 KDa were observed in 593 strain;specific band at 50 KDa was observed in 18 strain.The difference of protein expression amount in about 60KDa between 593 strain and 18 strain was observed.

结果：菌体可溶性蛋白电泳图中见593号菌株在75 KDa左右存在特异表达的蛋白条带，且二者在85、60、50、40 KDa左右蛋白表达量上存在差异；菌外可溶性蛋白电泳图中见593号菌株在75、40 KDa左右有特异表达的蛋白条带，而18号菌株在50 KDa左右有特异表达的蛋白条带，二者在60 KDa左右蛋白表达量上存在差异。

In addition, the developments of the capillary electrophoresis and chip electrophoresis applied to single-cell analysis in recent years are reviewed.

本文重点介绍作者研究组的工作，并对近三年来国内外在毛细管电泳及芯片毛细管电泳用于单细胞分析的新进展进行评论。

Composition of low molecular weight glutenin subunits and gliadins were investigated with thirty common wheat cultivars which are widely grown in Shangdong province. In our study we used acid polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis, analysed Sedimentation test, Protein content, Mixing time, Stability, Max-resistance to extension, Farinograph Quality Number and Extensogram area of the 30 common wheats. They have close relationship with the technology properties of noodle wheat cultivars.

本研究通过酸性聚丙烯酰胺凝胶电泳和SDS-聚丙烯酰胺凝胶电泳，分析了30个山东省种植面积较大且具有部分代表性的普通小麦品种的低分子量麦谷蛋白亚基和醇溶蛋白组成，测定了30个品种的蛋白质含量、沉淀值、面团形成时间、稳定性、粉质评价值、最大拉伸阻力和拉伸曲线面积等面粉及面团主要品质性状，分析了低分子量谷蛋白亚基和醇溶蛋白组成与面条小麦品质性状的关系。

Hole , and use a stuff to seal the middle hole before the electrophoresis; after product electrophoresis, takes down the stuff and enters the drying oven to roast, when roasting in the hole causes the paint the residual liquid flow to cake in the two ends of the surface.

电泳厂在产品电泳前需进行前处理工序，清除产品表面的垃圾和锈迹，由于产品中孔不允许有油漆，在电泳前将中孔用塞头封闭，产品电泳后取下塞头进入烘箱烘烤，在烘烤时孔内残留的液体流到两端面造成油漆结块。

MethodsCSF was extracted from patients with GBS and controls, and proteins were precipitated with icecold acetone. Isoelectric focusing electrophoresis was employed as the first dimension and sodium dodecylsulfatepolyacrylamide gel electrophoresis was employed as the second dimension.

方法分别取GBS疾病组和对照组的新鲜脑脊液，冰丙酮沉淀法提取总蛋白，进行第一向等电聚焦电泳和第二向十二烷基磺酸钠聚丙烯酰胺凝胶电泳分离蛋白质组，Image Master 2D图像分析软件对分析胶进行比较分析，识别差异表达明显的蛋白质点。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。