查询词典 electrophoresis

In order to reveal the differences among the dimorphic cells, basidiospores, arthrospores and mycelia of Tremella fuciformis were subjected to microscopical observation and electrophoresis analysis.

以银耳的担孢子、节孢子和菌丝体为材料，通过显微观察和电泳分析揭示银耳二型态细胞间的差异性。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

The molecular weight and distribution of samples were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.

用十二烷基硫酸钠一聚丙烯酰胺凝胶电泳方法确定3种样品的相对分子质量及其分布。

The efforts of different purification steps were checked by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate.

各步分离提纯的效果用SDS-PAGE证实。

The purified cTnⅠ was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.

纯化的cTnⅠ采用十二烷基硫酸钠－聚丙烯酰胺凝胶电泳和免疫印迹法进行鉴定。

The SDS-PAGE electrophoresis indicated that all engineered strains containing different expression plasmid could apparently express a 45 kD band specific for daunorubicin C-14 hydroxylase. The expression level of doxA gene was much higher when the doxA gene was under the control of promoter PermE. However, it was found that fd terminator had few effect on the expression of doxA gene.

SDS-PAGE蛋白电泳实验证明，上述五个菌株都能够表达大小约45 kD的柔红霉素C-14羟化酶蛋白，并发现以在PermE启动子控制下的doxA基因表达量相对较高，而终止子对＠中英文摘要 dd基因表达似乎没有影响。

The results dicate that there was nearly no difference from hibernation and activity period in all tissures except the liver and skeletal muscle of Elaphe rufodorsata as shown by electrophoresis plot, hibernation was lower than in activity period except that in liver of Erinaceus dealbatus, and LDH4 was not detected in all detected tissues of the three species of snakes.

结果表明：LDH同工酶的电泳图谱只有红点锦蛇的肝和骨骼肌中存在活动期和冬眠期的明显差异，在其余动物两个时期的组织中未表现出明显变化；刺猬的肝脏LDH活性在冬眠期显著地高于活动期，而其余的动物组织冬眠期LDH活性均显著低于活动期；三种蛇类组织LDH4均未见表达。

A flow injection system with electroosmotic driving, electrophoresis concentration and detection by ion-selective electrode has been presented.

提出一种电渗驱动，电泳富集和离子选择电极检测相结合的流动分析方法。

Second, the electroosmotic flows, which are becoming one of the most successful technologies of driving and controlling microfluids in capillary electrophoresis, were studied in this paper.

其次，本文对毛细管电泳分离中的主要的流体驱动方式—电渗驱动进行了深入地研究，建立了完整的电渗流数学模型，并采用涡量-流函数方法求解流场。

The appearance of capillary electrophoresis is a revolution in traditional electrophoretic technique.

毛细管电泳的出现是对传统电泳技术的一次革命，它结合了电泳的高效分离原理和色谱的毛细管柱制备方法与自动化操作技术，成为当。。。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。