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The DNA fragmentation of contusive tissuein rat presented a ladder break in electrophoresis after 6 hours. Thedegree and length of DNA fragmentation changed with injury time butwithout linear relationship.

大鼠脑挫伤组织DNA片段化6h以后出现梯状谱带，DNA片段化程度和片段大小随损伤时间而变化，但无明显线性关系。

Methods①The animal model of braincontusion caused by free drop hammer was established.②The injuredtissue of rat brain were stained by TUNEL for apoptosis,immunohistochemistry for Caspase-3 and Feulgen"s for DNA. Image analysistechnique and the statistical method were employed to explorate thetemporal changes of injury time.③The DNA was extracted from ratcontusive tissue of brain and assayed by gel electrophoresis toinvestigate the relationship between the DNA fragmentation and injurytime.④One handred and seventeen cases of death from traumatic braininjury were retrospective researched to investigate the characteristicof TBI in forensic medicine.⑤The contusive tissue of human brain werestained by TUNEL, Caspase-3 immunohistochemistry and Feulgen"s methodsin the same way to analyze and disclose the linear relationship betweentemporal changes with injury time.

方法①建立大鼠自由落体打击脑损伤模型；②对不同损伤时间组的大鼠脑挫伤组织进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色，结合图像分析技术和统计学分析，探讨脑挫伤后神经细胞凋亡、DNA片段化和含量的时序性变化；③提取大鼠脑挫伤组织DNA进行琼脂糖凝胶电泳分析，观察DNA片段化随损伤时间的变化特点；④对117例颅脑损伤致死案例进行回顾性分析，探讨其法医学特点；⑤选择不同损伤时间的人脑挫伤组织同样进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色和分析，观察上述指标与损伤时间的关系。

The zymogramatic variations of esterase isoenzyme and superoxide dismutase isoenzyme of 20 sample materials, including 5 species, 2 varieties, 1 multiploid of Isatis L. and 1 outgroup species, Coronopus didymus J. E. Smith, were analyzed with technique of polyacrylamide gel electrophoresis.

采用聚丙烯酰胺凝胶电泳，比较了菘蓝属5种2变种1多倍体品种及1外类群种共计20个样本的酯酶同工酶和超氧化物歧化酶同工酶的酶谱差异，并运用数量分类学的原理和方法对酶谱数据进行了聚类分析。

In order to clarify the rules of POD isoenzyme in the hazelnut, identify their blood relations between female, male and filial generations and select the superior parents for cross-breeding, we have studied the characteristics of peroxidase isoenzyme which are distributed in the 34 hazelnut varieties or lines by polyarclamide gel electrophoresis, The results showed that:POD isoenzymes of Corylus heterophylla Fisch, Corylus avellana L, and the hybrid of Corylus heterophylla Fisch × Corylus avellana L had obvious difference; some hybrids had lost P10 spectrum band of parents; the enzyme spectrum of 32 hybrid hazelnuts produced 'new hybrid spectrum bands'that could be considered as a mean to identify hybrids.

为了阐明过氧化物同工酶在榛品种中的分布规律，探讨平榛、欧榛及其杂种榛之间的亲缘关系，选育优势杂交亲本，本试验采用聚丙烯酰胺凝胶垂直电泳，研究了34个榛品种成熟叶过氧化物酶同工酶酶谱特征，结果表明：平榛、欧榛及其平欧杂种榛品种间酶谱具有较大差异；有的杂种品种遗失了父母本共有谱带P10；32个平欧杂种榛品种出现了双亲所不具有的新酶带，可作为鉴别杂种的一条途径。

Two-dimensional electrophoresis (2-DE) was used to study the variation expression of proteins of cucumber cotyledonary nodes induced with PP333. The characteristics and functions of differentially expressed proteins were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank.

应用双向凝胶电泳技术研究PP333处理后黄瓜离体子叶节培养物差异蛋白的表达，结合质谱分析和蛋白质数据库检索，确定所检出差异蛋白质的性质和功能。

Results Under the protection of VitC, the content of lower molecular weight protein of crystallin was increased significantly, and several LMW protein strap emerged in PAGE electrophoresis, while there were no significant changes in SDS-PAGE.

结论VitC对晶状体蛋白具有一定的抗氧化保护作用，初步得出今后在设计、验证和筛选药物对白内障的抗氧化保护作用时可依据的评价指标。

Objective To establish a method for fingerprints of pedicel of Cucumis melo by capillary zone electrophoresis.

目的 采用毛细管区带电泳法建立甜瓜蒂指纹图谱。

By means of the transmission electron microscope we found the typical morphology variation of apoptosis,such as the concentration and side-accumulation of the nuclear chromatin,the fragmentation of the nuclear and the concentration of the cytochylema etc.Cell cycle detected by flow cytometry showed one visible apoptosis peak (20.6%) in front of the peak of G1.The typical ladder was found in the 15 g.L-1 agarose gel electrophoresis of the nucleus DNA.

透射电镜下可见细胞核染色质浓缩、边集、核碎裂及胞质浓缩等凋亡细胞典型的形态学改变；流式细胞术周期分析显示在G1期峰前存在一个凋亡峰（20.6％）；核琼脂糖凝胶（DNA 15 g.L-1）电泳呈梯状。

The genome DNA was amplied and electrophoresis of Sichuan 7 native goat breeds and1 cultivated breed, including Tibet goat(n=27)、ChengDu Ma goat(n=20)、JinTang blackgoat(n=21)、LeZhi black goat(n=24)、JianChang black goat(n=23)、BeiChuan white goat(n=26),Jian Yang Daer goat(n=30) and NanJiang yellow goat (n=90), using 5 pairs of microsatelliteprimers.

选用5对微卫星引物，对四川7个地方山羊品种—简阳大耳羊(n=30)、建昌黑山羊(n=23)、成都麻羊(n=20)、藏山羊(n=27)、金堂黑山羊(n=21)、乐至黑山羊(n=24)和北川白山羊(n=26)及1个培育品种—南江黄羊(n=90)的基因组DNA进行PCR扩增，并进行电泳分析。

In order to reveal the differences among the dimorphic cells, basidiospores, arthrospores and mycelia of Tremellafuciformis were subjected to microscopical observation and electrophoresis analysis.

以银耳的担孢子、节孢子和菌丝体为材料，通过显微观察和电泳分析揭示银耳二型态细胞间的差异性。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。