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In this paper,the biological effects of ~(60)Co-γradiation to Bombyx mori and Antheraea pernyi were studied,and the changes of blood protein were observed through electrophoresis.

中文摘要本试验用~(60)Co-γ射线对家蚕和柞蚕生物学效应进行了研究，通过电泳试验证实了家蚕和柞蚕经辐射后体内血液蛋白质的变化。用~(60)Co-γ射线对家蚕卵进行照射，辐射剂量在10～30 Gy之间。

Leukocyte proteins concentrations were determined with Bradford Method. Phosphoproteins in the collected leukocyte proteins were enriched by BDTM Phosphoprotein Enrichment Kit. Finally the enriched phosphoproteins were separated by SDS-PAGE and dyed by argentation, and the electrophoresis map of SDS-PAGE was analyzed by Scion Image Tool.

刺激组加入表皮生长因子(40 μg/L)；抑制剂组加入细胞外信号调节激酶特异性抑止剂UO126 （10 μg/L）和表皮生长因子（ 40 μg/L）；对照组加入等量的培养基，用Bradford法测定细胞蛋白质含量，BDTM Phophoprotein Enrichment Kit分离富集白细胞磷酸化蛋白质，SDS-PAGE分离磷酸化蛋白质，银染法染色，采用Scion Image软件分析电泳结果。

The electrophoresis of DNA showed that lower dose (20Gy) irradiation made little influence on the DNA of armillaria,while higher dose(200Gy) irradiation resulted in a lot of breaks of DNA strands and these breaks mostly appeared in the 6 hours after irradiation and could be self\|repaired gradually.

DNA断裂主要发生在辐照后 6h之内，以后能自动修复

By the methods of acute toxicity test and single cell gel electrophoresis,this paper evaluated the toxicological effects of three veterinary drugs olaquindox,arsanilic acid and oxytetracycline on earthworm coelomocytes in vivo.

喹乙醇、阿散酸和土霉素是应用极广的兽药添加剂，在畜牧业的发展中起着特殊的作用，但其使用不当或过量使用会给环境带来很大危害。采用土壤中的重要生物赤子爱胜蚓作为生物材料，分别研究了喹乙醇、阿散酸和土霉素对赤子爱胜蚓的急性毒性和对赤子爱胜蚓体腔细胞DNA的损伤。

The slurry was inoculated on a rocking bed and continued for about 4 weeks, then arsanilic acid was determined spectrophotometrically (450 nm) and the genotoxicity changes of arsanilic acid on earthworm coelomocytes were tested with comet assay (single cell gel electrophoresis test, SCGE) in 0, 1, 2, 3, 4 weeks, respectively.

结果表明，阿散酸低浓度时在泥浆体系中能被较快降解，500mg/L的降解率达到了25%；蚯蚓体腔细胞彗星试验表明，所有降解液均具有遗传毒性，可导致蚯蚓体腔细胞DNA明显损伤，与对照相比具有显著性差异(P.05)。

Narrowbandoverlapped capillary electrophoresis signals of five arylamine mixtures have been studied by this method and satisfactory results are achieved.

应用于5个芳香胺类化合物的混合物的毛细管电泳窄锐重叠信号的处理，得到满意结果。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

In this paper, Attacus ricini nuclear polyhedrosis virus was studied. The molecular weight and pi of polyhedrin and structural polypeptides were determined by vertical slab SDS-PAGE and thin-layer isoelectrofocusing electrophoresis. The restriction pattern of ArNPV DNA was assayed by 5 restriction endonucleases.

本文较为系统的研究了蓖麻蚕核型多角体病毒(Attacus ricini nuclear polyhedrosis virus，简称ArNPV)形态特征和生化特性，并预测了多角体蛋白的二级结构，为开发利用蓖麻蚕核型多角体病毒这一生物资源提供了理论参考。

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料，分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA，并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

Two area of autolytic enzyme system appeared in SDS-PAGE electrophoresis map,it was suggested that at least two kinds of enzymes with different mole...

在温度与自溶酶活性的研究中发现，分子量较小自溶酶的耐热性明显优于分子量较大的自溶酶。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。