查询词典 electrophoresis

As a special branches of capillary electrophoresis CE , nonaqueous capillary electrophoresis NACE was variedly applied in the recent years .

非水毛细管电泳作为毛细管电泳法的重要分支已成为研究热点，近年来被广泛应用。

Serum immunoglobulin of Amur sturgeon Acipenser schrenckii, Chinese sturgeon Acipenser sinesis and Huso sturgeon Huso dauricus which were healthy and unimmuned, was first time respectively purified by using saturation ammonium sulfate precipitation followed by Sephadex G-200 gel column chromatography. Molecular weight and partial characterization of Ig and heavy chain and light chain of Ig were measured by polyacrylamide gel electrophoresis, SDS polyacrylamide gel electrophoresis, Western blotting and Ouchterlony method.

采用饱和硫酸氨分步沉淀和Sephadex G-200凝胶层析的方法，首次分别纯化制备了健康非免疫状态下中华鲟、史氏鲟和达氏鳇的血清免疫球蛋白，并采用聚丙烯酰胺凝胶电泳、SDS-聚丙烯酰胺凝胶电泳、蛋白免疫印迹及免疫琼扩实验等方法对其Ig及Ig亚单位的分子量和部分特性进行了分析。

The research displayed that yeasts Genus level can be discerned by agarose gel electrophoresis and polyacarylamide gel electrophoresis, but the latter is more advanced. The Saccharomyces cerevisiae strains ITS-1 sequences are most disalike, few of them are equal completely. The bigger differences are part of base pairs about Poly-T, others are exchanged by single base pair.

研究表明，以酵母菌株的rDNA ITS-1序列进行琼脂糖凝胶电泳，能把菌株分类鉴定到属的水平；采用非变性聚丙烯酰胺凝胶电泳能把部分酿酒酵母菌株分类鉴定到种的水平，区别效果较琼脂糖凝胶电泳有所提高；对rDNA ITS-1序列进行克隆、测序，经序列比对后发现，酿酒酵母属中酿酒酵母菌株的ITS-1序列大多数有差异，差异主要表现在ITS-1序列上游的一段Poly-T碱基上，其它处的差异仅是个别碱基的转换或颠换，ITS-1序列长度分布在360-370bp之间，G+C%含量均在35.3%左右。

Among the various electrophoresis technologies, the electrophoresis with a supporting media of polyacrylamide gel has become a common separate analysis method in lab and other fields, such as biochemistry research and clinical test, due to its steady chemical properties, excellent repeatability, small electroendosmosis, easy operation and high sharpness of separation.

在众多的电泳方法中，以高分子材料—聚丙烯酰胺凝胶为支持介质的电泳因其化学性质稳定、重复性好、电内渗小、操作简单及分辨率高等优点，已成为实验室常用的分离分析手段，在生化研究和临床检验等领域日益显示出其强大的生命力。

A novel method for the measurement of insulin-link growth factor I with capillary zone electrophoresis was developed. The molecular interaction between IGF-I and its polyclonal antibody was primarily studied with immunocapillary electrophoresis and the binding constant was determined. The work shows the possibility of applying CE to immuno-analysis, which could be a new clinical analytical method for diagnosis, treatment and monitoring for the endocritic and metabolic diseases.

以分子间相互作用理论为依托，以胰岛素样生长因子-Ⅰ及其抗体为研究体系，建立了用毛细管区带电泳测定IGF-I的方法，并首次用免疫毛细管电泳方法研究了IGF-I与它的多克隆抗体的相互作用，测定了它们的结合常数，进一步证实了在毛细管电泳中实施免疫分析的可能性，为内分泌及代谢性疾病等威胁人类健康的常见病的临床诊断及治疗观察提供了新的检测方法。

Methods By consecutive electrophoresis and nonconsecutive electrophoresis experiments, the best analysis conditions of chitooligosaccharide were determined.

通过连续电泳和不连续电泳对比实验确定壳寡糖的最佳分析条件。

The apoptosis induced by extract of Russula subnigricans Hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis.The result showed that agarose gel electrophoresis of DNA extracted from poisoned little white rat liver and kidney cellsrevealed typical 180 ~ 200bp integer-fold "ladder" bands.

亚稀褶黑菇提取液能诱导小白鼠肝肾细胞凋亡，亚稀褶黑菇提取液诱导肝肾细胞凋亡是亚稀褶黑菇中毒后机体病变死亡的重要表现形式之一 5。

The molecular weights of the two subunits were shown 142 KD for the larger one and 47 KD for the smaller one, respectively, on SDS—polyacrylamid electrophoresis. The molecular weight of the native vitellin was determined as 205 KD on native gradient polyacrylamid gel electrophoresis.

用蒸馏水沉淀法从东方粘虫Mythimna seperata新鲜卵中提取卵黄蛋白，SDS-PAGE分析结果显示，粘虫的卵黄蛋白由大小两个亚基组成，大亚基分子量为142KD，小亚基分子量为47KD，线性聚丙烯酰胺梯度凝胶电泳结果显示，卵黄蛋白的天然分子量为204KD。

Both the proteins components contained in haemolymph, fat body and ovary and the kenetics of the vitellogenin in haemolymph and ovary were analyzed by SDS—polyacrylamid electrophoresis, Ouchterlony plate tests and Rocket—immuno—electrophoresis. The results showed that the vitellogenesis initiated in adult stage and the Vg in haemolymph was detected firstly at 48h after eclosion. The titer of Vg in haemolymph raised rapidly in following 3 days and almost at the same time the vitellin appeared in oocytes. It was taken about 4—7 days after eclosion for the first eggs to grow up to the matured statics and to be laid out.

SDS-PAGE，双向免疫扩散和RIE分析粘虫生殖周期中血淋巴，脂肪体和卵巢中蛋白质谱带以及Vg滴度的变化，结果表明，Vg发生始于成虫期，Vg在血淋巴中的首现时间在羽化的24小时以后，以后连续三天血淋巴中的Vg滴度迅速上升，与此同时，开始沉积卵黄，卵母细胞逐渐发育长大，到成熟排出体外时大约历时4-7天。

Alteration and modification on two-dimensional electrophoresis in Arabidopsis thaliana , such as improvements in protein extraction, lyses buffer, adding thiourea, provide a better separation result. This paper has an optimism of the experimental conditions on two-dimensional electrophoresis.

通过对拟南芥双向电泳技术过程中的适当改进，如蛋白质的提取与溶解方法、上样量和聚丙烯酰胺凝胶浓度，加入硫脲，硫代硫酸钠等，对拟南芥双向电泳技术进行了优化，提高了双向电泳图谱的蛋白质点数目与分辨率。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。