查询词典 electrophoresis

The capillary electrophoresis fingerprints of Fructus Forsythiae was established to control its quality. The background electrolyte was a 75 mmol/L sodium borate solution adjusted to pH 9.7 with 0.1 mmol/L NaOH solution. The detection wavelength was 228 nm and a voltage of 14 kV was applied. The Forsythia suspensa Thunb.

采用毛细管区带电泳法，以75 mmol/L硼砂溶液(用0.1 mmol/L氢氧化钠溶液调pH 9.7)为背景电解质，运行电压14 kV，检测波长228 nm，重力进样15 s(高度9 cm)，建立了连翘药材的毛细管电泳指纹图谱。

A novel method is reported for mass separation of proteins, based on sodium dodecyl sulfate polyacrylamide gel electrophoresis.

报导了一种基于十二烷基硫酸钠聚丙烯酰胺凝胶电泳的新型蛋白质质量分离方法。

The optimal separation for capillary electrophoresis is 20% methanol, and 0.13mM of β-cyclodextrin in 25mM sodium tetraborate buffer solution(pH 9.1), and 20℃of temperature.

实验结果显示最佳化的毛细管电泳分离条件为25mM四硼酸钠溶液(pH= 9.1)，添加0.13mM β-cyclodextrin 及20%甲醇溶液。

In so doing, we expected to obtain the information regarding particle size and size distribution of AuNPs directly from the electrophoresis results. In this experiment we used 10mM Sodium tetraborate and 10mM Sodium phosphate containing 200mM Sodium dodecyl sulfate as electrophoretic run buffers (pH 8.8), and AuNP Standards were treated with the synthesized compound hexyl-oligo(p-phenyleneethyny-lene)-poly as the sample and separated by CE.

根据电泳最佳条件的实验结果，我们采用10mM Sodium tetraborate与10mM Sodium phosphate并添加200mM Sodium dodecyl sulfate作为缓冲溶液（pH 8.8），以经实验室合成的界面活性剂（hexyl-oligo (p-phenyleneethyny-lene)-poly，Hex-OPE-PEO）修饰过后的金奈米粒子标准品作为样品进行电泳实验，将所测得的电泳迁移率与标准品颗粒大小做一校正曲线，可以得到最佳的电泳迁移率－粒径大小之线性关系（R2＞0.99）。

We found when the pH value of washing buffer was 7. 4, the first apex of curve corresponded the solution of purified IgG whose purification was identified with discontinuous on different concentration polyacrylamide electrophoresis.

实验中发现洗脱液pH值为7.4时洗脱第一峰即为纯化的IgG，用不连续缓冲系统聚丙烯酰胺凝胶浓度梯度电泳鉴定层析洗脱下的IgG达到电泳纯。

RNA quality and quantity were determined by spectrophotometer,electrophoresis and RT-PCR.

TRIZOL是近年来常用的一种从组织细胞中快速提取总RNA的试剂。

For example, Zhejiang Changshan Longchang Electron Co., Ltd, after self-research and development, has produced electrophoresis glass passivation chip. Zhejiang Changshan Insulation Material Co., Ltd, by bring in the environmental protection copper clad laminate technique, adopting KD-F exhaust gas incinerator, calling back lots of flammable mixed gas produced in the production process, finally makes the energy source callback ratio meet 96%, standard coal saved 1200T per year, and the environmental protection spiritualization ratio above 99%.

如浙江常山隆昌电子有限公司自主研发并投产的电泳法玻璃钝化芯片；浙江省常山绝缘材料有限公司引进国内关键环保型覆铜板的无卤技术，采用KD-F废气焚烧炉，回收生产过程中上胶系统产生的大量可燃性混合气体，能源回收率达96%，年节约标准煤1200T，环保净化率达99%以上。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

Keywords Capillary electrophoresis;on-line sweeping concentration;hollow fiber-based liquid-phase microextraction;fluorescence spectroscopy;interaction;serum albumin;Strychnos alkaloids;fangchinoline;tetrandri

毛细管电泳；在线推扫富集；中空纤维液相微萃取；荧光光谱法；相互作用；血清白蛋白；马钱子碱；防己诺林碱；粉防己碱；槲皮素；卡马西平；甲基多巴；左旋多巴

Methods: NGF was prepared from mouse submaxillary glands by the way of elution with CM 52 column. The molecular weight and purification of NGF were detected by SDS-PAGE polyacrylamide gel electrophoresis.

以小鼠领下腺为原料，採用CM52多次洗脱的力法分离神经生长因子，SDS-PAGE电泳检测NGF的分子品质和纯度，採用鸡胚背根神经节培养方法检测NGF的生物活性。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。