查询词典 electrophoresis

Phosphoprotein enrichment technique combined with two-dimensional gel electrophoresis is an effective approach to study phosphoproteome, laying a foundation for further investigation of human kidney phosphoproteins.

磷酸化蛋白富集技术与二维凝胶电泳技术的结合是研究磷酸化蛋白质组的有效方法，为进一步研究人肾组织磷酸化蛋白奠定基础。

Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562，采用MTT法检测细胞增殖活性，单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象，Annexin V FITC/PI双标法检测细胞凋亡，Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

The photosensitization effects of trisruthenium- on DNA caused by visible light has been studied by using of agarose electrophoresis.

采用琼脂糖凝胶电泳研究了以可见光引发的三联吡啶钌与DNA之间的光敏反应。

Amplification products were detected by agarose gel electrophoresis and clustering analysis by PHY...

RAPD分析技术可作为真菌系统分类的客观而有效的手段之一。

At least three clear bands were obtained by sodium dodecyl sulfate polyacry- lamide gel electrophoresis, a subunit and 13 subunit of phycoerythrin, y subunit of phycoerythrin and one subunit of R-phycocyanin.

SDS-PAGE电泳鉴定至少有三条明显的带，为PE的α和β的重叠带，γ亚基和RPC的亚基。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Results Capillary electrophoresis detected the 1/600 of the PCR product from 20 picogram of male DNA, as amount of DNA in 3～4 cells.

放射性同位素标记可提高检测灵敏度，但操作复杂、且受一定的条件限制。

The band shapes of hemoglobin electrophoresis were different between high altitude pika and rat.

据此报导基础上，西藏医科所＊］又在西藏三个不同海拔地区，对移居、世居以及只进人高原三个月的人群作了血红蛋白类型检查。

Based on literature, applications of electrochemical analysis methods, including coulometry, polarography and high performance capillary electrophoresis, in the determination of flavoniods are described to offer reference to the research, development and application of flavonoid compounds.

摘要在查阅文献的基础上，介绍了黄酮含量的电化学测定方法，包括库仑滴定法、极谱法、高效毛细管电泳等，旨在为黄酮类化合物的研究、开发、应用提供参考。

The effects of pourpoint depressants on the wax crystal s of Shengli crude oil are studied by using X-RAY diffraction, the polar microscope and micro-electrophoresis instruments.

取得以下主要结论：从原油中分离出的蜡晶为正交晶型的晶体，晶面距为0。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。