查询词典 electrophoresis

The detection cell was evaluated by the determination of galactose, glucose, and fructose in capillary zone electrophoresis.

为了考察该检测池的分析性能，采用毛细管电泳安培检测技术分离分析了葡萄糖、果糖和半乳糖，结果令人满意。

The influence of various factors on amplification outcome was observed by gelose gel electrophoresis.

目的探索多重PCR体系中各成分的最适组合，以便在真菌性角膜炎的诊断中获得准确、可靠的结果。

To evaluate the safety of the DNA vaccine pVAX1/F1-V against plague, the purity of plasmid DNA pVAX1/F1-V was detected by SDS-PAGE and gelose electrophoresis method; pasmid DNA was detected by PCR in tissues of BALB/c mice immunized with the plasmids intramuscular injection.

为了研究鼠疫DNA疫苗pVAX1/F1-V质粒的安全性，试验运用SDS-PAGE电泳和琼脂糖电泳等方法检测了鼠疫DNA疫苗pVAX1/F1-V质粒的纯度，并以小鼠为动物模型进行了质粒pVAX1/F1-V的急性毒性和长期毒性试验，用PCR方法检测外源基因在小鼠组织中的分布情况，用ELISA、ELISPOT方法检测了其自身的免疫反应。

Calcium chloride modified nanoparticles prepared by means of chemistry. Analyse the combination of calcium phosphate nanoparticles and DNA, the protection to DNA as well by gelose gelatin electrophoresis. When the green fluorescence protein gene was regarded as report gene, gene vector of calcium phosphate nanoparticles transfected CNE-2 cell in vitro and vivo. Combine the calcium phosphate nanopartides and suicide gene yCDg1yTK for Nasopharyngeal Carcinoma therapy in vitro.

化学方法制备，氯化钙修饰纳米颗粒，用琼脂糖凝胶电泳分析磷酸钙纳米颗粒与DNA的结合效率及对DNA的保护作用，用绿色荧光蛋白基因作报告基因，将磷酸钙纳米颗粒为基因载体转染鼻咽癌(CNE-2)细胞和在动物体内转染肿瘤细胞；以及将磷酸钙纳米与自杀基因yCDglyTK结合，并在体外对鼻咽癌进行基因治疗。

It was found that TPS4-1 was a polysaccharide-protein-nucleic acid conjugate by using the methods of UV, IR, NMR, HLPC, GC, gelose electrophoresis, periodate oxidation, smith degradation.

通过吸附柱层析、离子交换柱层析和葡聚糖凝胶柱层析等多级层析纯化的方法获得了茶多糖的相对均一组分TPS4-1。

Result: 1.(1) Three strips come to appear in the position of 6.0kb, 5.4kb and 600bp after gelose electrophoresis and their size accord with recombined plasmid DNA, pcDNA3 plasmid and VEGF165 gene accordingly.(2) Pure degree of the pcDNA3-VEGF165 recombined plasmid is: OD260/OD280=1.87. Its

在进行了重组质粒的扩增、提取、纯化、鉴定、兔MSCs的原代及传代培养以及RT-PCR、Western Blot 转染检测后，实验取得了令人满意的结果，证实了MSCs 能在体外有效转录外源VEGF 基因并分泌出VEGF 蛋白，同时也说明pcDNA3-VEGF165重组真核表达质粒是有效的。

The ORF2 gene is the most important protein-code gene for porcine circovirus, and is the main gene differing porcine circovirus typel from porcine circovirus type2 (PCV2). To construct a specific PCR detecting method, we designed a pair of primers to amplify a partial fragment of ORF2 gene from PCV2, referring to fifteen strains complete genome published in Genbank. The PCR product was about 0.67kb in size detected by gelose gel electrophoresis.

猪圆环病毒的开放阅读框2(ORF2)是其主要结构蛋白的编码基因，也是区分猪圆环病毒1型(PCV1)和猪圆环病毒2型(PCV2)的重要基因，本研究参照GenBank已发表的15株PCV2基因序列，设计合成一对引物，对PCV2的ORF2基因进行PCR扩增，产物经琼脂糖凝胶电泳，呈现一条大小约0.67kb的特异条带，经条件筛选，建立了猪病料中PCV2感染的PCR检测方法。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

The result of seed gliadin electrophoresis showed that there were significant differences in the zynome between transgenic lines and receptor, which were appearance of new bands and disappearance of the receptor's and the degree of expression was also different.

醇溶蛋白电泳结果显示，转基因株系与受体相比存在着显著的差异，主要体现在转基因后代中出现了新带或者缺少了受体的部分谱带及表达量的差异。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐