查询词典 electron-volt

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The morphology and microstructure characteristics of the apertured silver doped TiO2 microtubes were characterized by means of the X-ray powder diffraction, thermal analysis, transmission electron microscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and N2 absorption.

氮吸附的结果显示该样品具有很高的比表面积，银的修饰没有显著影响二氧化钛微管的孔结构； XRD的表征结果显示，银修饰的二氧化钛多孔微管具有良好的锐钛矿晶型； XPS的分析结果表明银以游离的金属态存在； TEM的观测结果显示，银团簇沉积于二氧化钛纳米晶粒的表面。

Assimilatory nitrate reductase is a soluble, multiple center redox enzyme that catalyses the two-electron reduction of nitrate to nitrite using pyridine nucleotide as a electron donor.

植物中的硝酸盐还原酶(nitrate reductase，NR)作为限速酶，催化硝酸盐还原为亚硝酸盐，这一过程在氮代谢中处于关键地位，对植物生长发育、产量形成有重要影响。

In theory, firstly, the numerical simulation methods, such as the boundary element method, the finite difference method and the finite element method, were utilized to calculate the distribution of electric field intensity and potential of the electron-gun system, the focusing system and the whole equipment, respectively. Then, the trajectories of the electron-beam were traced by using of the Runge-Kutta method and the Monte-Carlo method associatively. The point spread function was also calculated.

在理论分析中，先后采用边界元方法、差分方法、有限元方法等科学数值计算方法，编制程序，对X射线源的电子枪发射系统、聚焦系统以及发射系统与聚焦系统的组合系统等的电场分布进行了严格的计算，在准确求得各系统电场内各个剖分点的电场场强、偏导数等参量的基础上，采用蒙特-卡罗模拟方法和不等距龙格-库塔方法相结合，追踪由LaB_6单晶阴极表面发射出的大量电子束在电场内的运动轨迹，求出点扩展函数，并根据点扩展函数的优劣，反复调节、大量计算对比，挑选出X射线源最佳的电极结构及其组合。

The main task of this project is to design a bus adapter, which will be one part of the aviation electron system, to connect the embedded aviate route controller to the aviation electron bus.

该项目的主要任务是通过设计一总线适配器将嵌入式航路控制器接入航电总线，使之成为航空电子系统的一部分。

In the Presence of uncoupling reagents water stress had little effect on the photosynthetic electron transport in mesophyll cells of Iwpoea batatas, but in the absence of uncoupling reagents it promoted the electron transport and displayed pronounced uncoupling effect.

在有解偶联剂存在时，水分胁迫对叶肉细胞的光合电子传递没有影响，但在无解偶联剂存在下，水分胁迫促进了甘薯叶肉细胞的光合电子传递，表现出明显的解偶联效应。

The results have indicated that the introduction of electron-donating methoxy or electron-withdrawing cyano-group at the 7-position of 3-phenylcoumarins caused a bathochromic shift for both absorption and fluorescence spectra. The phenyl group at the 4-position derivative has a hypsochromic shift for both absorption and fluorescence spectra.

结果发现，在3-苯基香豆素的7-位引入给电子基团甲氧基或吸电子基团氰基均使它们的吸收光谱和荧光光谱产生红移，4-苯基香豆素的衍生物的吸收光谱和荧光光谱均产生蓝移。3-苯基香豆素衍生物与4-苯基香豆素的衍生物的基态和激发态的电子转移方向相反。

Owing to the inclusion complexation, the benzoquinone as the electron acceptor of reduced GOD in the enzyme-containing polymer membrane has higher concentration, thereby faciliating the electron transfer of the immobilized enzyme.

由于分子包合作用，作为电子受体的苯醌在含酶的环糊精聚合物膜中具有较高的浓度，从而加速了固定化酶的电子传递。

The bimolecular electron transfer rate constants demonstrate a marked dependence on ionic strength. This indicates that the electrostatic interaction plays significant role in the electron transfer.

电子传递速率常数的对数与离子强度符合指数关系，表明静电作用在电子传递过程中起着较重要的作用。

In order to solve the backward electron transfer problem, 9 kinds of rhenium bipyridyl photosensitive dyes with different electron donating groups on the p or m-position of the pyridine rings, 2 kinds of rhenium bipyridyl and 2 kinds of ruthenium tris-bipyridyl photosensitive dyes with two tyrosine ethyl ester groups on the bipyridyl rings were designed and synthesized.

本文针对该类太阳能电池中存在的电子回传问题，设计合成了在吡啶配体的间位和对位带有不同供电基团的9种铼联吡啶光敏染料，在联吡啶配体上带有两个酪氨酸基团的2种铼联吡啶和2种钌三联吡啶光敏染料。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。