查询词典 effect a junction

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Remember that the base-emitter junction of a BJT is nothing more than a PN junction, just like a diode, and that the "diode equation" specifies how much current will go through a PN junction given forward voltage drop and junction temperature

其实，BJT的发射结不过是个PN结，就如同二极管。因此流过PN结的电流与其正向压降的关系也符合"二极管方程"

Damsite selection of Yilan Navigation Hydro- junction on Songhuajiang River;2. Study on the general layout scheme for reinforcement and extension project of Guoyang junction sluice;3. The river reach of Dadingzishan Navigation-power junction engineering is characterized by wide grade II terrace,large distance between the two dikes,thick covering of river bed,and strong movability and permeability of riverbed,and the overall layout is the essence of construction of low-head navigation-power junction on the reach.

松花江大顶子山航电枢纽工程河段具有Ⅱ级台地宽、两岸堤距大、河床覆盖层厚、河床质可动性及渗透性强等特点，该河段兴建低水头航电枢纽工程其总体布置是关键，利用正态物理模型(11∶00)及遥控自航船模等研究手段，对大顶子山航电枢纽工程预可、工可、初步设计及施工图设计等阶段枢纽总体布置进行了多方案比选，提出枢纽主要建筑物布置：自右岸依次为船闸、10孔泄水闸、电站、28孔泄水闸及土坝。

This text analyzes the concept of the environment geology, settle the geology data by survey and the history data of junction area of Hydroelectric Power Station. elaborate the basic geology characteristic of dam area especially junction area, analyze the problem of geology environment in junction area concluding protogenic geological environment and protogenic geological environment. analyze the Physical geology phenomenon such as collapse and mudflow...etc. predict the probability evoked by earthquake in junction area.

本文通过对环境地质概念的辨析，对泸定水电站枢纽区踏勘所得的地质资料和历史资料进行了整理分析，阐述了泸定水电站库坝区特别是枢纽区的基本地质特征，着重分析了枢纽区的环境地质问题，包括原生地质环境及次生地质环境，对枢纽区的物理地质现象如滑坡、泥石流等做了简单的论述分析，并简单预测了枢纽区可能诱发地震的概率。

The results are indicated that only the strength of gap junction is large enough, can the abnormal oscillation propagate to synchronize the other neurons around and the process is chaotic and complex;the nonlinear and complexity of the oscillations with strong strength of gap junction are larger than that of oscillation with weak strength of gap junction.

结果表明：只有电突触耦合达到一定强度时，异常振荡才能传导并引起神经元的同步振荡；传导活动是混沌和复杂的；在突触耦合强度较强时的神经元活动的非线性和复杂性要强于其在突触强度较弱时的。

Three results are received:(1) The population of neurons can synchronize when the strength of gap junction is large enough and the process is chaotic and complex;(2) The nonlinear and complexity of the seriously disorder oscillation are larger than that of slightly disorder oscillation in a 2-D network;(3) The nonlinear and complexity of the oscillations with strong strength of gap junction are larger than that of that with weak strength of gap junction.

结果表明：电突触达到一定强度时神经元群才能同步振荡，这种活动是混沌和复杂的；同一网络中异常节律较严重的神经元活动的非线性和复杂性要强于异常节律较轻微的神经元；突触耦合强度较强时的神经元活动的非线性和复杂性要强于其在突触耦合强度较弱时的。

In recent years, the company focus on developing solar junction box and connectors products, current products ranging from 5W to 500W and so a full range of sections of the junction box, and developed specifically for second-generation thin-film modules using junction box, our products are by TUV certified and meet the RoHS directive, and apply to a variety of backplane, and TPT, TPE, PYE and glass and so on back to match.

近年来，公司重点开发太阳能接线盒和连接器产品，目前产品已涵盖从5W到500W等全系列的各款接线盒，并开发出专供第二代薄膜组件使用的接线盒，公司产品已通过TUV认证并符合RoHS指令，且适用于多种背板，与TPT、TPE、PYE以及玻璃等多种背板相匹配。

Each bypassing circuit is connected in parallel with a junction formed by the input and second terminals of an associated one of the transistors to limit a voltage across the junction when the junction is reverse biased.

每个旁路电路与由相关的一个晶体管的输入端和第二端子形成的结并联连接，以便当该结被反向偏置时限制该结上的电压。

The anode structure of CI-LIGBT is optimized by numerical simulation, and it is pointed out that segmented p〓 anode region surrounded by n〓 anode region is the key to improve the ability of current limiting, and the more deeper the n〓 anode junction relative to the p〓 anode junction, the more powerful the ability of current limiting is. The affection of the breakdown voltage BV〓 of the reverse biased p〓n〓 junction and the sample resistance upon the latching current, the latching voltage and the on-state voltage have been studied, and it is indicated that the tradeoff between the on-state voltage and the latching characteristics is the excellentest when BV〓 is 1. 1V so that its on-state voltage can be just only 0.6V higher than conventional LIGBT. It is also found that this device is especially suit to high temperature environment. Based on X. B. Chen's surface voltage sustaining structure using optimized variational lateral doping and Ludikhuize's multiple floating rings structure, high voltage LIGBT and is studied in this paper.

通过数值模拟，优化了CI-LIGBT的阳极区结构，指出采用被n〓阳极区包围的单元状p〓阳极区是提高该器件限流能力的关键，而n〓阳极区结深相对p〓阳极区结深越大，器件的限流能力越强；分析了反偏p〓n〓结击穿电压BV〓和取样电阻对器件闩锁电压、闩锁电流和导通压降的影响，得出BV〓在1.1V时器件导通压降和抗闩锁能力间的折衷关系最优，其导通压降仅比普通LIGBT高0.6V；分析了温度对LIGBT闩锁性能的影响，表明CI-LIGBT即使在450K的高温下也不会闩锁，尤其适用于高温工作环境。

Based on many previous studies of the Buqin Mountain, A′nyêmaqên and Kuhai-Sêrtang ophiolitic mêlange zones in the junction between the two orogenic belts and through further survey and isotope chronological study, the authors find that the three ophiolitic mélange zones are not independent but form a typical triple junction with respect to the composition, textures and structures, geometry and isotope chronology, i.e. the Qinling-Kunlun triple junction.

本文在前人对发育于两造山带衔接区的布青山、阿尼玛卿以及苦海—赛什塘3条蛇绿构造混杂带大量研究的基础上，通过进一步的调查以及同位素年代学研究发现，这3条蛇绿构造混杂带并非相互独立存在，它们在物质组成、结构构造、几何形态、同位素年代学特征上构成较为典型的三向联结构造，即秦—昆三向联结构造。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？