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Recombinant colony, E.coli DH5α(pMG320),expressing O\|antigen of V.cholerae O 139 was detected from the bank by immunological agglutinative reaction.

经鉴定分析重组克隆所表达的O 抗原具有良好的免疫原性及反应原性。酶切分析质粒 pMG32 0 ，推知其O 抗原基因大小约 4 6kb。

Suppose C is a Graph with k loops andf: G 梸- G is a continuous map with at least a peri- odic point. We prove the following three conditions are equivalent:(1) f is equicontinuous;(2) One of the following End + 2k + 1 conditions holds: in FJm.~0(E~+2L!= flJ1,m = l,2,挃,End J=1 + 2k; 1 0 = flf is a ioop on Gandf I~:0?- 0 is conjugate j=1 to a rational rotation;(3) One of the following End + 2k + 1 conditions holds: k m Lfl.m/~oE~(G+2i!~ is uniformly convergent, m = 1,2,,End+2k; n There is a poistive integer number n,~~~ is uniformly convergent.

假设G是含有k个圈的图，f是G到自身上的连续映射，并且至少有一个周期点，我们证明了下列三条陈述是等价的：（1）f是等度连续的；（2）下列End+2k+1个条件之一成立： m）F l）O=是G的一个圈，且fl_o：O→O共轭于一个有理旋转；（3）下列End+2k+1个条件之一成立： m）是一致收敛的，m=1，2，…，End+2k； l）存在某个正整数n，是一致收敛的。

S Benko, Protestants, Catholics and Mary; L Bouyer, The Seat of Wisdom; EA Carroll,"A Survey of Recent Mariology," MarS 18, and "Theology on the Virgin Mary: 1966 - 1975," TS 37; JB Carol, Fundamentals of Mariology and Mariology; H Graef, Mary: A History of Doctrine and Devotion; R Laurentin, The Question of Mary; G Miegge, The Virgin Mary; TA O'Meara, Mary in Protestant and Catholic Theology; O Semmelroth, Mary, the Archetype of the Church; E Schillebeeckx, Mary, Mother of the Redemption.

s benko ，新教徒，天主教徒和玛丽；升bouyer ，所在地的智慧；环境卡罗尔，"统计调查结果显示，最近mariology ，"火星18 "神学对圣母玛利亚： 1966年-1 975年，" 6月2 4日t s3 7； j b卡罗尔基本面mariology和mariology ； h graef ，玛莉：历史，理论和奉献精神； r洛朗坦，问题的玛丽；克miegge ，圣母玛利亚；电讯管理局局长o'meara ，玛丽在新教和天主教神学； o泽梅尔罗特，玛丽，原型教会电子商务施雷贝克，玛丽的母亲赎回。

Coil O85 antigen contained 8 varieties of genes, including UDP-Nacetylglucosamine-2-epimerase gene (f1). UDP-galactopyranose mutase gene, glycosyl transferase genes (f3, f5, f6, f8), O-antigen transferase gene and O-antigen polymerase gene, in which two genes, wzx and wzy and 4 pairs of primers were identified to be specific to E.

发现8个开放阅读框架并确定功能，分别为：UDP-N-乙酰葡萄糖－2-异构酶基因，吡喃型UDP-半乳糖变位酶基因，糖基转移酶基因（orf3、orf5、orf6和orf8），O-抗原转运酶基因和O-抗原聚合酶基因。

The finest music - in the two sonatas with obbligato harpsichord in B minor and A major, and in two with continuo alone, in E minor and E major - is top-drawer Bach , probably composed in the 1730s, teeming with melodic invention designed to put the newly fashionable instrument through its paces.

最好的音乐-在这两个奏鸣曲o bbligato古键琴与B小调和重大，在连续两，仅在E小调和E大-是最重要的巴赫，可能组成的1 7 30s，到处是旋律发明设计把时髦的新仪器通过其步伐。

In this article two groups of Shigella and E. coli strains that have identical O-antigen structure were studied, S.

同时本文还对另外一组有同样O-抗原结构的鲍氏志贺氏菌8和大肠杆菌O143的O-抗原基因簇进行了分析。

The complete fusion excitation function for the 16O+24Mg reaction has been measured in the energy range of 55～90MeVwith the step of 1.0MeV using a position sensitive△E-E telescopy system.

本工作用位置灵敏的△E－E望远镜系统，入射能量步长为1.0MeV，测量了55～90MeV~（16）O＋~（24）Mg反应全熔合激发函数。

Firstly, the reaction thermodynamics was analyzed. Then, the reaction mechanism was studied, and the results are as following:① Ca moves to the surface of Dy〓O〓 by vapor phase;② Dy〓O〓 reduced to Dy by Ca is fast;③ Dy moves to the Fe surface mainly by vapor and various Dy-Fe alloys are produced gradually;④ The forming process of DyFe〓 may be described by Contracting Core Model;⑤ The rate-controlling step of the reaction is the Dy diffusing toward contracted and nonreaction nuclear center Fe.

首先考察反应的热力学可能性；其次探讨反应机理；通过实验确定：①Ca主要以气态形式迁移到Dy〓O〓表面参与反应；②Dy〓O〓被Ca还原为Dy的速度很快；③Dy主要以气态形式迁移到Fe表面逐级生成Dy-Fe合金；④DyFe〓的形成过程可以应用收缩核模型来描述；⑤Dy通过DyFe〓产物层向缩小的未反应核中心Fe的扩散是反应的控速步骤，通过动力学计算确定：还原扩散反应的表观活化能E〓=45kJ/mol。

At last,a convergent and general protocol to marine natural product,as demonstrated by the enantioselective total synthesis of malyngamides O,P,Q,R, 5"-epi-Q and 5"-epi-R bearing terminal vinylic chloride structure,was developed in detail.E,(7S)-7-methoxytetradec-4-enoic acid was prepared using the above described Johnson-Clainsen rearrangement,the terminal vinyl chloride was constructed using Wittig reaction as key step,the pyrrolidone derivative was constructed using L-serine as natural chiral pool.Starting from the synthesis of malyngamides O and P to explore the general route,an aldol reaction of methyl acetate with the vinyl chloride part in the presence of LDA gave the skeleton of amine portion,followed by amidation with the fatty acid finished the synthesis of malyngamide P.

先以malyngamides O和P为试点探索通用合成路线，将乙酸甲酯和烯氯部分在LDA存在下进行aldol反应得到氨部分的骨架，再将手性不饱和脂肪酸和氨部分进行酰胺化反应完成了malyngamide P的合成，将P在碱性条件下进行烯醇甲基化反应完成了malyngamide O的全合成。

Once a member of the rather anonymous group,"World Class Wreckin' Cru", Dre earned himself a name by producing tracks for Eazy-E, the D.O.

Dre曾经是一个并不知名的组合&World Class Wreckin' Cru&的成员，当他得到了为Easy-E、the D.O.C以及其他艺人或团体创作歌曲的机会后，他成名了。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。