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Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

将获得的DC与用 E-花环分离脐血单个核细胞得到的T淋巴细胞共同培养3 d，然后分为两组，实验组2继续与不同比例的HO9810细胞共同培养3 d后获得细胞毒T淋巴细胞，对照组2除加入HO9810外，还加入了无关细胞，用四甲基偶氮唑蓝法检测其对HO9810细胞的抑制作用。

Earlier on the 19th, out in the Baltic west of Dago, covering German battlecruiser 'Moltke' is torpedoed in the bow and slightly damaged by British submarine 'E-1'(Lt-Cdr Laurence) in her first success with the Baltic flotilla.

此前的19日，在波罗的海西部的达戈，英国潜艇"E-1"在加入波罗的海舰队其第一次的成功战绩，其中包括轻伤德国战列巡洋舰"毛奇"的舰首。

S. price . So , so you can actually order *** it over e-Bay at half the price including customs duty , so that actually puts quite ***. a dampener on the whole thing .

所以，事实上你可以在e-Bay网上以包括海关税在内的半价订购，而这样的情况却实际上对整个事件造成了一定的阻碍。

It is concluded that Daniell Cell is thermodynamically irreversible and that criterion of ΔrGm is not equal to that of E.

讨论了Daniell电池的热力学可逆性问题和电化学反应的判据问题，认为Daniell电池是热力学不可逆电池，电化学反应的ΔrGm判据与E判据并不等

In 1762, he negotiated for the post of Kapellmeister to the court of Darmstadt ; although he protracted the negotiations for reasons that are opaque to historians and did not actively take the post, he nevertheless was appointed "Hofkapellmeister of Hessen-Darmstadt", a title he used in the dedication of his Harpsichord Concerto in E minor.

在1762年，他谈判的Kapellmeister的职位，其达姆施塔特法庭，虽然他长期的理由是不透明的历史学家，也没有积极参与后的谈判，但他被任命为"Hofkapellmeister黑森州，达姆施塔特"的称号他用他的古键琴协奏曲E小调奉献。

The best of comprehensive efficiency between natural lighting and heat insulation is Low-E glass, the best performance of natural lighting is clear glass, but performance of heat insulation is bad, the best performance of heat insulation is green semi-reflective glass, average daylight factor is about 1% over, 0.7% of residential standards, but its illuminance is not suitable about 200 Lux.

平均昼光率与室内玻璃表面温度标准差之比值的综合效能评估中，Low-E玻璃为采光与隔热综合效能最优异之材质，而清玻璃为采光效能最优异之玻璃材质，但清玻璃隔热效能不佳，绿色半反射玻璃的隔热效果最好，而采光方面的昼光率平均为1%，采光虽然还符合住宅的昼光率标准0.7%，但照度普遍不佳，平均室内照度约200 照度。

E-Sullivan is a device for the deaf-blind.

e-Sullivan 是一个为聋哑人设计的服务。

Low-income women are often deficient in a number of micronutrients, including vitamins C and E, vitamin B complex and folate.

低收入妇女往往有缺陷，在一些微量营养素，其中包括维生素C和E ，复合维生素B和叶酸。

Objective Rapeseed oil deodorizer distillate is an important source of natural vitamin E.

目的菜籽油脱臭馏出物是天然维生素E的重要来源之一。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。