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Results The difference between flying cadets and aircraftman in N score was statistically significant, but not in P,E,L score. The cadets from city in E scores were higher than those from countryside, but lower in L scores. Education level and occupation of cadet's parents affected E and L scores of cadets.
结果 飞行学员的N分明显低于普通新兵,差异有显著性,而P、E、L分差异无显著性;来自城市的飞行学员较来自农村的飞行学员E分高,L分低;父母受教育程度及从事职业对飞行学员E、L有一定影响。
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E XP returns the value of e raised to the power of X, where e is the base of the natural logarithms.
E XP 返回e的X次幂的值,其中e是一个自然对数基底。
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Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.
maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a,构建重组质粒 pET-20b-bglA 和 pET-28a-bglA,然后转化不同大肠杆菌 E.coli 宿主,Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达,重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA),可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA),比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA),这些结果表明,E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因,分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别,有助于提高该酶在 E.coli 中的表达;进一步优化诱导条件,重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养,IPTG 浓度由 1000 μM 降至 25 μM,目的蛋白可溶性表达由 12.3%增至 32.8%,提高 1.7 倍,16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达,较 37 ℃下诱导培养提高 1.2 倍。
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The spawning ground of filefish covers a wide area from 25°30′ N to 30°00′N and 122°00′E to 126°30′E according to the distribution of the spawners, hatchedlarvae and juveniles and catch in spawning season, the area mainly covered from25°45′N to 27°00′N and 122°00′E to 123°30′E.
从产卵个体、仔稚鱼和产卵盛期渔获量的分布判断东海绿鳍马面鲀的产卵场在25°30′—30°00′N、122°00′—126°30′E海区,范围较广,主要产卵场在25°45′—27°00′N、122°00′—123°30′E,水深100—120米的海区。
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Normal working hours, B2-B1 between the need to add a positive voltage, E-B1 between the PN junction forward biased if the Canadian single-junction transistors that turn, after turn-on, E-B1 between the resistance will soon be reduced, to B2-B1 between the current rapid increase in current increase, so that the resistance between the E-B1 further reduced, so the formation of a strong positive feedback.
正常工作时,B2—B1间需加正电压,E—B1间的 PN结若加正向偏置,单结晶体管即导通,导通后,E—B1间的电阻会很快降低,使B2—B1间的电流迅速增加,电流的增加,又使E—B1间的电阻更进一步的减小,如此形成了强烈的正反馈。
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Look at this ,it's our latest product. Y:I don't like something recommended,generally not good. E:Why not having a look? Now let me introduce its supernatural function to you.This style of hair drier have two wind-speed ajustment,includes the quick wind one and the natural cold one. Hot wind can dry your hair ,and cold wind can keep it still. Any style you want ,just use it. Y:I want to use it to blow my boy friend's hair looks like yours . E:OK,I'll show its function to you. Y:Wait,wait...How can I see the effect while your hair is dry? E:How do you feel like it? Y:I'm satisfied with it.
你看看这款这是我们的最新产品 Y:我不喜欢别人推荐的,一般都不是好东西 E:百闻不如一见现在就让我给你介绍一下它的神奇功能这款吹风机具有快速风和自然冷风两档风速调节,热风吹干冷风定型你想让你的头发成什么型它就能吹出什么型 Y:我想给我的男朋友吹头发,大概你这么长,发质和你差不多 E:为了展示我们这款吹风机的神奇功能现在就由我为你亲自示范 Y:等等等等,,,,你的头发都是干的我怎么能看到效果呢 E:你觉得怎么样?
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While 30μmol·〓 FRCRSFa and 10μmol·〓 E-4031 were dialysed intracellularly via the patch-pipette, FRCRSFa significantly decreased 〓 exchange current and there was no the further decrease after adding FRCRSFa in the perfused solution with the same concentration, whereas E-4031 dialysed had no effect on 〓 exchange current, but a obvious increase of it occurred when adding E-4031 to the perfused solusion with the same concentration.
分别将30μmol·〓的FRCRSFa和10μmol·〓的E-4031加入微电极内液中,FRCRSFa显著抑制了〓交换电流,当在细胞外灌流液中加入同样浓度的FRCRSFa时无进一步抑制;而胞浆内的E-4031对〓交换电流无显著作用,而在灌流液中加入同样浓度的E-4031后,却显著增强了〓交换电流。
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(1)In NSCLC, the overexpression of Ezrin and the abnormal expression of E-cadherin were correlative with primary carcinoma with metastatic carcinoma. The expressions of Ezrin and E-cadherin in primary NSCLC tissues and in metastatic carcinoma of lymph nodes were different. All of these suggested that the overexpression of Ezrin and the abnormal expression of E-cadherin may promote tumor metastasis. The investigation of Ezrin and E-cadherin may be useful for offering one method to prevent tumor from metastasizing.
(1)在NSCLC中,Ezrin的过度表达与伴有癌转移的原发性NSCLC有关,并且在淋巴结转移灶中的过度表达高于原发灶;E-cadherin的异常表达与伴有癌转移的原发性NSCLC有关,并且在淋巴结转移灶中的异常表达高于原发灶;以上均提示Ezrin过度表达和E-cadherin异常表达可能促进肿瘤转移,对Ezrin与E-cadherin的研究可能有助于提供一种阻止NSCLC患者肿瘤转移的治疗措施。
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Both 1 and E neurons could be further subdivided into 4 subtypes,the PS neurons into E-I and 1-E type. In the 153 RRNs recorded in rats, 68 were I (44. 5肠),55 E(35.9%),3 0 PS(19. 6%).It was found that a group of E-I PS neurons that fire preceding phrenic nerve respiratory activity are located in the mNRF,which was believed to be important in the generation of the basic respiratory rhythm.
100,包括E-1和1-E。在大鼠所记录到的153个RRNs中,吸气神经元68个(44.5%,呼气神经元55个(35.9%,呼吸跨时相神经元30个(19.600),在mNRF分布有较多的呼气一吸气跨时相神经元,这类神经元放电总是先于隔神经吸气性放电,可能在基本呼吸节律发生中起重要作用。
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Finally we show that any r.e.degree is splittable with an r.e.infimum over any lesserr.e.degree in the class of d-r.e.degrees.
最后我们证明了任意r.e。度可在任一更小的r.e。度上分裂且有下确界。此结果不同于Lachlan在r.e。
- 相关中文对照歌词
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- 推荐网络例句
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Do you know, i need you to come back
你知道吗,我需要你回来
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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.
1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。
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Chapter Three: Type classification of DE structure in Sino-Tibetan languages.
第三章汉藏语&的&字结构的类型划分。