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Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

将获得的DC与用 E-花环分离脐血单个核细胞得到的T淋巴细胞共同培养3 d，然后分为两组，实验组2继续与不同比例的HO9810细胞共同培养3 d后获得细胞毒T淋巴细胞，对照组2除加入HO9810外，还加入了无关细胞，用四甲基偶氮唑蓝法检测其对HO9810细胞的抑制作用。

Earlier on the 19th, out in the Baltic west of Dago, covering German battlecruiser 'Moltke' is torpedoed in the bow and slightly damaged by British submarine 'E-1'(Lt-Cdr Laurence) in her first success with the Baltic flotilla.

此前的19日，在波罗的海西部的达戈，英国潜艇"E-1"在加入波罗的海舰队其第一次的成功战绩，其中包括轻伤德国战列巡洋舰"毛奇"的舰首。

S. price . So , so you can actually order *** it over e-Bay at half the price including customs duty , so that actually puts quite ***. a dampener on the whole thing .

所以，事实上你可以在e-Bay网上以包括海关税在内的半价订购，而这样的情况却实际上对整个事件造成了一定的阻碍。

It is concluded that Daniell Cell is thermodynamically irreversible and that criterion of ΔrGm is not equal to that of E.

讨论了Daniell电池的热力学可逆性问题和电化学反应的判据问题，认为Daniell电池是热力学不可逆电池，电化学反应的ΔrGm判据与E判据并不等

In 1762, he negotiated for the post of Kapellmeister to the court of Darmstadt ; although he protracted the negotiations for reasons that are opaque to historians and did not actively take the post, he nevertheless was appointed "Hofkapellmeister of Hessen-Darmstadt", a title he used in the dedication of his Harpsichord Concerto in E minor.

在1762年，他谈判的Kapellmeister的职位，其达姆施塔特法庭，虽然他长期的理由是不透明的历史学家，也没有积极参与后的谈判，但他被任命为"Hofkapellmeister黑森州，达姆施塔特"的称号他用他的古键琴协奏曲E小调奉献。

The best of comprehensive efficiency between natural lighting and heat insulation is Low-E glass, the best performance of natural lighting is clear glass, but performance of heat insulation is bad, the best performance of heat insulation is green semi-reflective glass, average daylight factor is about 1% over, 0.7% of residential standards, but its illuminance is not suitable about 200 Lux.

平均昼光率与室内玻璃表面温度标准差之比值的综合效能评估中，Low-E玻璃为采光与隔热综合效能最优异之材质，而清玻璃为采光效能最优异之玻璃材质，但清玻璃隔热效能不佳，绿色半反射玻璃的隔热效果最好，而采光方面的昼光率平均为1%，采光虽然还符合住宅的昼光率标准0.7%，但照度普遍不佳，平均室内照度约200 照度。

E-Sullivan is a device for the deaf-blind.

e-Sullivan 是一个为聋哑人设计的服务。

I finally read their e-mail in detail, and she had an upset stomach in Dec!!

我最后又仔细读了一下e-mail，她的胃在12月就不行了！

Low-income women are often deficient in a number of micronutrients, including vitamins C and E, vitamin B complex and folate.

低收入妇女往往有缺陷，在一些微量营养素，其中包括维生素C和E ，复合维生素B和叶酸。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。