查询词典 dystrophin

2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点（44、45、49和50号内含子以及5'端启动子区的序列）设计引物，在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾：CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身，经高分辨率的变性聚丙烯酰胺凝胶电泳后，利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置，进行扫描分析，确定产物片段的大小。

Dystrophin may have a novel role in localizing nNOS to sarcolemma and regulating the expression of nNOS.

与dystrophin、α、β、γ和δ-sarcoglycan检测同时分析，发现nNOS缺乏与dystrophin缺乏有关，而与SG的改变无关。

Dystrophin protein plays a key structural role in muscle fiber function by connecting sarcolemma with the intracellular actin filaments.

dystrophin蛋白次通过连接与细胞内肌动蛋白丝肌膜肌纤维中的功能键结构的作用。

Our results indicate that the deficiency of dystrophin would lead to the reduction or deficiency of SG proteins, which would cause disruption/dysfunction of dystrophin-glycoprotein complex , loss of linkage between subsarcolemmal cytoskeleton and extracellular matrix which, in turn, would lead to sarcolemma instability and eventually to muscle cell necrosis.

表明dystrophin缺乏将导致包括SG在内的dystrophin相关蛋白的显著减少，DGC复合体结构破坏或功能障碍，细胞膜下骨架与细胞外基质连系的丧失，致使肌细胞膜不稳定，在肌肉收缩时容易受损，最终肌纤维变性、坏死。

They must be spliced precisely to produce an uninterrupted fragment of dystrophin protein.

他们必须精确拼接产生一个不间断的dystrophin蛋白片段。

In Cases 3, 4 and 5, complete absence of dystrophin inmunoreactivity was found.

在第三，四与第五病童，完全没有Dystrophin的免疫反应活性。

Methods The clinical and laboratory data was analyzed in 40 children with confirmed diagnosis of Duchenne muscular dystrophy by dystrophin detection.

方法对40例经dystrophin蛋白检测确诊的DMD患儿的临床特征及实验室检查资料进行分析。

There were no correlation between expression of dystrophin and severity of muscle pathology changes.

结论检查dystrophin在肌纤维膜上的表达对DMD具有特异性诊断价值，但临床病理改变的严重程度主要与年龄和病程有关。

The association between nNOS and dystrophin may take part in mediation of signal transduction in muscle cell.

说明dystrophin对于nNOS定位在肌膜上，以及对nNOS表达水平的调节具有重要作用。

Increases in dystrophin expression were shown in muscle biopsies from a number of the boys participating in the trial.

在大约百分之15的裘辛型的男孩，dystrophin基因突变产生起作用的告诉肌肉细胞在成足够长的时间前就产生一个信号，停止dystrophin蛋白质产生。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失