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Methods Lymphatic endothelial cells were isolated from pig thoracic duct, primary cultures and subculture were finished by collagenase digestion method, immunocytochemstry was used in identification of their biological characterization.

用胶原酶灌注消化的方法,从猪胸导管中分离出淋巴管内皮细胞进行原代及传代培养,应用免疫细胞化学法对培养的细胞进行鉴定。

The thoracic duct endothelia in vitro could remain their biological properties, and could be made a tool for studing their functions and cancer metastasis mechanism by lymphatics. The positive expression of VEGF-C in cancer cells was correlated with cancer development, it seem to be that VEGF-C induce lymphoangiogenesis through its receptor Flt-4. The Flt-4 expression of lymphatics in cancer tissues was assosiated with cancer development, it is considered that there are new lymphatics in cancer tissues.

体外培养的大鼠胸导管内皮细胞可以保持体内淋巴管内皮细胞的生物学特性,可作为研究大鼠淋巴管内皮细胞功能和进一步研究癌转移机制的体外实验材料;大鼠大肠癌组织腺体的VEGF-C表达与肿瘤进展有关,推测其通过受体Flt-4诱导淋巴管生成;Flt-4在淋巴管的阳性表达随肿瘤进展增多,推测在癌组织内存在淋巴管的新生。

The preventive ligation of thoracic duct during resection of esophageal carcinoma can indeed reduce or eliminate the incidence rate of the chylothorax after operation.

术中行预防性低位胸导管结扎术可明显减少甚至消除食管癌患者术后乳糜胸的发生。

Methods The author summed up the indications of selective low thoracic duct ligation to prevent the chylothorax after esophagectomy.

目的 探讨选择性低位结扎胸导管预防食管癌术后乳糜胸的发生。

Cisterna chyli ; Thoracic duct ; Magnetic resonance imaging ; Lymphography

乳糜池;胸导管;磁共振成像;淋巴显像

Objective To observe the morphological changes of pancreatic tissue of thoracic duct ligated rats in fine and ultrastructural levels, and to determine whether lymph block animal model can affect pancreatic islet amyloid polypeptidedeposit in rat pancreas.

目的 观察胰淋巴淤滞动物模型胰组织结构变化和胰淀肽沉积情况,探讨淋巴淤滞对胰组织细微和超微结构的影响。

Methods The rat thoracic duct endothelia were cultured and the morphology observed and identified by TEM and Factor Ⅷ-related antigen immunohistochemically. Wistar rats were used to make the animal model of primary colorectal cancer, the cancer type were appraised by HE staining and PAS Reaction. The expression of VEGF-C and its receptor Flt-4 and ICAM-1 in the cancer tissues were carried through immunohistochemistry staining and In situ hybridization.

通过体外培养大鼠胸导管内皮细胞和构建大鼠原发性大肠癌动物模型,观察内皮细胞的形态和生长状态,用透射电镜和Ⅷ因子相关抗原进行鉴定,用HE染色和过碘酸雪夫氏反应区别癌组织的类型和期别,用免疫组织化学方法和原位杂交方法研究内皮细胞和大肠癌组织的VEGF-C及其受体Flt-4和ICAM-1的表达。

In order to overcome this disease hospital used Onyang Tongluo, Blood Circulation, adjusting business health, and foster the good series of proprietary Chinese medicines, and the thoracic duct drainage combined with treatment, the results showed that patients from Disiwu days ,周身skin began to soften, physical function and visceral functions are significantly improved, edema disappeared, immune function is basically back to normal.

为攻克这一顽症,该院采用温阳通络、活血化瘀、调整营卫、扶正祛邪等系列中成药,又与胸导管引流术结合起来进行治疗,结果显示,患者从第四五天起,周身皮肤就开始变软,肢体功能和内脏功能得到显著改善,水肿消失,免疫功能也基本恢复正常。

The thoracic duct ligation has blocked chylomicrons to blood, diminished the formation of LDL, after all and made the absorption and metabolism of lipid abnormal.

在食管癌根治术中结扎胸导管,阻断了乳糜微粒入血,导致LDL生成减少,对脂类的吸收代谢有不良影响,因此主张脂肪以外的营养物质可经肠内供给,而脂肪营养则从静脉供给,从而促进病人的康复。

Based on the factors that cause the chylothorax after esophagectomy, we selected 40 esophageal cancer patients with radical surgery and played the operation of selective low cluster thoracic duct ligation.

根据食管癌术后并发乳糜胸的原因,总结低位结扎胸导管预防食管癌术后乳糜胸的适应证,选择40例食管癌根治术患者进行选择性行低位集束结扎胸导管。

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