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France Muscovy duck ; Hatching ; Calcium ; Phosphorus

法国番鸭;孵化;钙;磷

The Ultrastructure changes of liver, heart, lung, kidney, spleen, bursa and thymus of Muscovy ducks infected experimentally with muscovy duck reovirus were observed.

对人工感染番鸭呼肠孤病毒发病雏番鸭的心、肝、肺、肾、脾脏、胸腺、法氏囊等7种实质器官的超微结构进行了观察。

By determining the calcium content,phosphorus content and their proportion in the eggshells of France Muscovy duck in different hatching stages,the changes of calcium and phosphorus contents in its eggshells in different incubation stages were studied.

方法通过对不同孵化时期法国番鸭蛋壳内钙、磷含量及比例进行测定,研究了不同孵化阶段蛋壳内钙、磷含量的变化。

Key words: Muscovy duck parvovirus;goose parvovirus;PCR;differential diagnosis

番鸭细小病毒;鹅细小病毒;聚合酶链式反应;鉴别诊断

Hexon protein gene of egg drop syndrome virus, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp.

运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒 E05株中分段扩增六邻体蛋白基因,分别与克隆载体pMD18-T连接后,转化感受态细胞,对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序,结果表明,E05株六邻体蛋白基因长度为2733 bp,共编码910个氨基酸。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞,获得重组杆状病毒rBV-σC;SDS-PAGE和Western-blot分析表明,在sf-9细胞中表达了分子质量约37ku的σC蛋白,该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应,这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较,同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系,及其免疫原性的变化规律,通过DNA重组技术,设计了一对引物LHMP7/LHMP8,该对引物选取位于2885~2900及4618~4604的两段序列,跨幅为1734bp,并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点,分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增,并将PCR产物克隆到pMD18-T载体上,分别得到二个重组子:pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

Is specialized in bonsai nursery stock; corn, wheat, beans; pig, cow, lamb, chicken and ducks conventional; turkey, silky, free-range chicken, muscovy duck farm production, such as the mu.

南陵县春秋农牧有限公司是专门从事花卉苗木盆景;玉米、小麦、豆类;猪牛羊、常规鸡鹅鸭;火鸡、乌骨鸡、土鸡、番鸭等生产的百亩农庄。

Effect of rice-duck mutualism on nutrition ecology of paddy field and rice quality.

稻鸭共作对稻田营养生态及稻米品质的影响。

Rachel plans a big date with a special dinner, new lingerie and a nice dress; while eating dinner at her place, the chick and the duck upset Joshua, who has a phobia of farm birds; since his apartment is being remodeled and his parents are out of town, they go to his parents' house; unexpectedly, his parents return home while Rachel is wearing only a negligee; she tells them it's actually a new dress and ends up having to wear it while they all go out to dinner.

瑞秋盛装赴乔西亚的约会:她穿着新的内衣和新的裙子;两人在她家用餐时,小鸡小鸭出现了;乔西亚素来惧怕家禽,于是说,我父母今天都不在,不如到我父母家怎么样;早归的乔西亚的父母正好撞见瑞秋只穿了性感内衣;急中生智的瑞秋慌称那是今年新款的裙子,结果她只好穿着内衣和乔西亚一家去餐厅吃饭。

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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。

If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。