查询词典 dropped

After the first blind date, she dropped to see the cowherd at the rendezvous for a few times.

经过第一次有意被安排见面后，织女与牛郎在指定地点又被安排见过几次面。

The rope would have come back up and dropped into the crevasse.

绳子会上来，然后又掉到裂缝里

If you dropped your watch in a crevasse near the top, the glacier would drop it in the ocean in about a thousand years.

如果将你的手表扔进山顶的裂缝中，1000年后冰川将带着它滑入海洋。

The state's crime rate also dropped substantially during that time.

在此期间该州的犯罪率也有大幅的下降。

He just dropped some crocodile tears for his wife ,then got married as soon as possible.

他假惺惺地为妻子的死掉了几滴眼泪，然后，很快又结婚了。

On my last day in the city, July 18, I almost dropped my croissant when I opened the paper and saw a report about me.

在那个城市的最后一天，应该是7月18日，当按例打开报纸时，我惊了，手里美味可口的羊角面包几乎掉下来，那上面赫然有关于我的一篇报道。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

She dropped a cube of sugar into her tea and stirred it with a spoon .

他将一方糖放入茶中，用勺子搅拌。

The Ca〓 in cumulus cells dropped at first and returned to normal level when progesterone was used.

两者均可使卵母细胞内的Ca〓水平略微下降。

Recently, international crude already fell to about 110 dollars / bucket, than the most exalted 140 much dollars / the bucket dropped to exceed 10%; Cupreous price also 62000 yuan from first half of the year / ton, drop to in August 59000 yuan of the middle ten days of a month / ton.

最近，国际原油已跌至约110美元/桶，比最高位140多美元/桶下跌了超过10%；铜价也从上半年的62000元/吨，跌至8月中旬的59000元/吨。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。