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The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

Garnish by floating a lemon slice or drop in a twisted lemon peel.

配菜的浮动柠檬片或减少扭曲的柠檬皮。

Be in " tank battle " in, the player will direct a tank and enemy confront the tough with toughness, every tank has tree of distinctive science and technology each, apply the experience value that gets on battlefield to develop battle skill, kill the hostile tank that acts by other player, drop their triumphal foothold mark, every a group of people of same interest has the tank of 3 kinds of become terror-stricken at the news making a person to be able to choose, resembling is tank of Tartarean cat of leopard type tank, M18 or Xueman tank...

在"坦克战"里，玩家将指挥一辆坦克和敌人硬碰硬，每台坦克各有独特的科技树，运用战场上得到的经验值来发展战技，杀死由其他玩家扮演的敌对坦克，降低他们的胜利据点分数，每个阵营都有三种令人闻风丧胆的坦克可以选择，像是豹式坦克、M18地狱猫坦克或雪曼坦克…等，所以玩家可以依照自己的风格，选择适合的坦克，虐杀小兵、支配战场，看著敌对玩家惊惶逃开。

The drop in buy-back activity highlights the defensive stance adopted by US companies in the face of a liquidity squeeze and the increased probability of the economy slipping into recession.

回购活动的下降，凸显出美国企业在面对流动性紧缩和经济衰退可能性上升时所采取的防御性姿态。

Using the spatula as a guide, drop the dough by spoonful into a 2.5-inches wide and 18-inches long log directly onto the parchment paper. Slightly flatten it to 1/2-inch thick. Leave 2 inches space between logs.*** Brush eggwash over logs.

用刮刀做指引，一大勺一大勺地把面团直接舀到油布上，使它形成一个长18英寸（45cm），宽2.5英寸（6.3cm）的圆柱体，然后轻轻压扁成1/2英寸（1.3cm）厚。

Using the spatula as a guide, drop the dough by spoonful into a 2.5-inches wide and 18-inches long log directly onto the parchment paper. Slightly flatten it to 1/2-inch thick. Leave 2 inches space between logs.

用刮刀做指引，一大勺一大勺地把面团直接舀到油布上，使它形成一个长18英寸（45cm），宽2.5英寸的圆柱体，然后轻轻压扁成1/2英寸厚。

Because breast cancer patient is faced with,be mixed to accept treatment for cancer by examine double blow, bring about its not to believe or reject the thing related to the disease to the disease, adopt escape, reject an attitude, and the negative effect that the disease brings (the fear of adversary art, lay eye, change cure lose one's hair, of sweetheart passion drop), often bring a patient heavy psychological pressure, and it affects a patient to seek again about the information related to the disease and ability, still meet those who cause behaviour degrade and of cure interrupt.

由于乳腺癌患者面临着被诊为癌症和接受治疗的双重打击，导致其对疾病的不相信或拒绝和疾病相关的事物，采取逃避，拒绝态度，而疾病带来的负面影响(对手术的惧怕，世俗的眼光，化疗脱发，爱人激情的减退)，常带给患者沉重的心理压力，而它又影响患者寻求有关和疾病相关的信息和能力，还会造成行为的退化以及治疗的中断。

The longer there is no solution, the greater the risk that the lunatic fringe, abetted by the likes of Iran's president, may one day manage to drop a bomb on the Jewish state.

达成解决方案的时间拖得越久，受伊朗总统之流唆使的极端主义者可能某一天在犹太国家引发恐慌的危险就越大。

Some individual entrepreneurs and internet businesses may be able to get by with a "mail drop" address, which means simply that your mail is delivered to your prestigious London address and then forwarded to your physical address, often your home.

一些个体企业家、互联网业务可能得到了一个"降邮件"地址简单说，就是你的邮件送到你的地址，然后送交伦敦著名你身体地址常贵的家。

chinese animated films had a glorious history, but to enter the twenty-first century, after the eighties, with the trend in hot pursuit of the audience and love to the traditional approach based chinese art films gradually been discarded by the younger generation 80 more after the drop in foreign works such as man-day.

内容摘要：中国日本曾有过辉煌的历史，但进入二十动漫卡通八十年代之后，随着日本对潮流的追捧和热爱，以传统手法为主的中国美术片逐渐被人们所日本动漫卡通片，新生一代80后更受落于日漫等外国作品。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐