查询词典 down

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Besides wonderful games,the "Wine Down" also has two advantages:one is covering the presider of club,Zhang Xin.

精彩的不止是游戏，整个"Wine Down"还有两个亮点：一是对俱乐部主席郭欣的采访。

This step involves top-down design and stepwise decomposition,using pseudocode or flow charts.

这一步涉及Top-Down设计，并逐级分解，用伪代码或流程图。

David shares the viral message Slow Down FAST and helps people raise the roof on all facets of their lives without risking implosion.

David在Slow Down FAST中分享了病毒信息，帮助人们抱怨生活的各方面，而不是自我封闭。

It interprets a scripting language that allows applications or administrators to provide the ACE Service Configurator framework with commands, called directives , to locate and initialize a service's implementation at run time, as well as to suspend, resume, reinitialize, and/or shut down a component after it's been initialized.

他解释了脚本语言允许程序或者管理员在运行时提供ACE Service Configurator framework命令，调用指令，来定位和初始化服务的实现，例如挂起，恢复，重新初始化，shut down一个部件在他初始化后。指令可以用两种方法指定

To beat down to a state of ruination or degradation.

Pro-的意思是forward，在这里意为'down'，后来逐渐转到了人的行为这一层次。

Reverb drenched guitars on tracks like So Alive, Hopesablaze, and Shark Attack are reminiscent of mid 80s Big Country or synth-pad era Rush, yet Down and Above's urgent shirtsleeve lyrics take you to a more intimate place.

现在这三个好友组建了明尼阿波利斯的乐团"Down and Above"要用他们真诚的音乐来讲述他们的故事……征服宇宙啦、喷射火箭啦等等等等

His stump speech ties the emergency to deregulation and "trickle-down economics", the outgoing doctrines.

奥巴马在政治演说中，将金融突发危机归咎于放松管制和被奉为开支教条的"涓滴经济学"(trickle-down economics)。

However, upper airway obstruction is encountered after palatoplasty with certain craniofacial abnormalities (e.g., Treacher Collins syndrome, Turner's syndrome, Pierre-Robin syndrome, and Down's syndrome, etc.).

上呼吸道阻塞会在颚修补术后，一些特定颅颜畸型的病人发生（如Treacher Collin症侯群、Turner's症侯群、Pierre-Sequence症侯群、Down症侯群等）。

I came back to my hotel tired, wash face and lay down for sleep.

我回来了到我的旅馆疲倦的，洗涤面孔和位置睡眠的down。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。