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The method is based on the formation and deformation of double-stranded DNA membrane on a gold surface, which can encage reporter molecules between the gold surface and the double-stranded DNA, or keep them away from the gold surface.

致密的双链DNA分子层可以将电化学信号分子禁闭在金表面和双链DNA之间，或者将信号分子与金表面隔离开，使其无法接触裸金面。

The Jiayue Bridge in Chongqing is an extradosed bridge with double Y-shape pylons,double cable planes and with a main span 250 m.

重庆嘉悦大桥是一座双塔双索面Y形矮塔斜拉桥，桥梁主跨跨径250m，主桥箱梁采用单箱单室大悬臂结构形式，介绍大桥总体设计思路。

Zhili CUTTING MACHINERY's facty area funded in WANJIANG industrial district, DONGGUAN. ITS products including double-cylinder with four columns double pillars cutter, sptive plastic cutter, automatic rocker cutter, level cutter, all kinds of making shoes line.

智力机械在大陆的生产基地设在东莞万江，产品包括精密四柱油压裁断机、吸塑包装裁断机、自动送料裁断机、直切机、平切机、发泡机、上胶机、吸塑机、平面式裁断机、摇臂裁断机、龙门油压裁断机、铆钉机、折边机及皮鞋生产线等。

Suddenly the sky thunder rumbled overhead of rain Who knows my heart Love is like a dream, who are deaf I really shouldn't she enchanted Love a person to love shouldn't I always double containing sacrifice That may not be still force yourself to go on The heart is frowsty more boring supports the cold heart You know I only love you Your attitude I cannot balance I love, I cannot help wanting to ask too deep I really can't match him there Gray sky see yesterday lamp What were you thinking Love vaguely You don't know how to say Live like the wind candles Love a person to love shouldn't I always double containing sacrifice That may not be still force yourself to go on The heart is frowsty more boring supports the cold heart You know I only love you Your attitude I cannot balance I love, I cannot help wanting to ask too deep I really can't match him there You know I only love you Your attitude I cannot balance If you love need two points I think I don't want to wait You know I only love you Your attitude I cannot balance I love, I cannot help wanting to ask too deep I really can't match him there You know I only love you Your attitude I cannot balance If you love need two points I think I don't want to wait

突然，天空雷声隆隆开销雨谁知道我的心爱是一场梦一样，谁是聋子我实在不应该，她陶醉爱一个人爱不应该我总是含有双重牺牲这可能不是还强迫自己继续下去心脏是frowsty更无聊支持冷酷的心你知道，我只是爱你你的态度我不能平衡我爱，我也忍不住想提出过深我真的无法比拟他在那里灰色的天空见昨日灯你是怎么想爱依稀你不知道如何说活得像蜡烛的风爱一个人爱不应该我总是含有双重牺牲这可能不是还强迫自己继续下去心脏是frowsty更无聊支持冷酷的心你知道，我只是爱你你的态度我不能平衡我爱，我也忍不住想问一问太深我真的无法比拟他在那里你知道，我只是爱你你的态度我不能平衡如果你爱的需要两点我想我不想等待你知道，我只是爱你你的态度我不能平衡我爱，我也忍不住想提出过深我真的无法比拟他在那里你知道，我只是爱你你的态度我不能平衡如果你爱的需要两点我想我不想等待

Summer is double foot when loving to give an issue most, the environment of fuggy moisture, add perspire mix more the addition with sufficient metabolic ministry, return those who have the high-heeled shoes of fine heel to persecute, make the most easily double foot loses health.

夏天正是双脚最爱出问题的时候，闷热潮湿的环境，加上出汗多和足部新陈代谢的增加，还有纤细鞋跟的高跟鞋的迫害，最易令双脚失去健康。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

Genesee strives to establish its core product pipelines in antibodies and recombinant antigens to meet the needs of a wide variety of diagnostic techniques, such as latex agglutination assay, capture ELISA, double antigen ELISA, double antigen-mediated lateral flow immuno-chromatograph, chemoluminescence, multiplex microsphere assay.

公司致力于抗体和重组蛋白两种核心原材料产品的开发，以满足各种诊断技术的需求，如乳胶凝集试验法，间接法ELISA，双抗原ELISA，双抗原介导的胶体金快速诊断，化学发光法，蛋白液体晶片多重诊断技术。

Black Square Table clothing Napkins VestWorking Cleanning Ladies Polo/t-shirt, Black trousers Nurse shoes white Long sleve shirt green Black ties Black lace Name tag Carpets Double Bed cover Double bed Duvet RECEPTION/LOBY/INTERNET ROOM BOUTIQUE Reception Counter Key holders and other Ther utensils for reception Furniture for the Lobby Undercounter bar fridge Cupboards for Lobby bar Counter + Shelfs for boutique Set of 3 posts + 3 CHAIRS VARIOUS Pictures for walls Eletrical guiser 50L Split aircon 9000BTU Splip aircon 12000 Split aircon 18000 Split aircon 24000 Fluorescent lamp arm Portable computer lap top Trolle for luggage Telephone Central w/100 extentions + 10 exterior lines Fire dectectors for 70 rooms Piano automatic for pub Security chains for room doors Water spray Massage table Automatic chair for massages

黑色 表服装广场餐巾纸 VestWorking清洗夫人马可波罗/ T恤，黑色长裤白色护士鞋龙绿色球衣sleve 黑色领带黑色花边名称标签地毯一张双人床封面双层床上用品接待/·罗比/互联网室专卖店接待柜台钥匙持有人及其他有接待用具家具的大厅 Undercounter酒吧冰箱碗柜的大堂酒吧反+ Shelfs的精品集3个+ 3椅子不同图片墙 Eletrical guiser 50L 斯普利特空调9000BTU Splip空调12000 斯普利特空调18000 斯普利特空调24000 日光灯臂便携式计算机圈的顶端（ med.price ）特罗勒行李电话中央w/100扩张+ 10外墙线消防的70间客房自动钢琴的酒吧安全链的房间门喷水按摩表自动按摩椅供

Specific massage means is: Double buccal, gyroidal by massage outside introversion; The skin between eyebrow, by fall to be played up batch; Area of nose lip channel, light graze is wiped; Frontal ministry chuck is attacked, by wipe to both sides close lightly in the forehead; Canthus arc is brushed; Eye week circle is kneaded; Buccal week circle is kneaded; Mandible skin appropriate is played batch; Finally, peck kowtow and flap double buccal ministry.

具体按摩方法是：双颊，螺旋形由内向外按摩；眉间皮肤，由下向上弹拨；鼻唇沟区域，轻轻擦抹；额部轻叩击，由额中向两边抿抹；眼角弧形擦抹；眼周圆形揉抹；口周圆形揉抹；下颌皮肤宜弹拨；最后，啄叩和拍打双颊部。

Notably, compounds 12–14 can be described as a 3D open–framework with nano–channels, compound 15 can be described as a 3D open–framework with double helical channels, while compound 16 can be described as a 3D heterochiral coordination polymer with double helical channels, which can be obtained by spontaneous resolution from the racemic phosphonic acid.X–ray powder diffraction analysis shows that compound 16 is a pure phase. Surface photovoltage spectroscopy of compound 16 indicates that it possesses positive SPV response in the range of 300–600 nm and shows p–type semiconductor characteristic.

值得注意的是，化合物12-14为具有一维纳米孔道结构的三维开放骨架结构，化合物15是具有双螺旋孔道的三维开放骨架结构，而化合物16是外消旋的有机膦酸配体通过自发拆分而形成的具有双螺旋孔道的多手性三维开放骨架结构。X-射线粉末衍射分析表明化合物16为纯相；化合物16的表面光电压光谱分析中，在300-600nm范围内有3个正的光(来源：ABC论文12网www.abclunwen.com)伏响应带，表明它具有p型半导体的特征；化合物16的诱导表面光电压谱进一步确定它的p型半导体的特征；磁性研究表明，化合物16中两Mn原子之间存在弱的反铁磁相互作用。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。