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The detectable group, biotin-ε-aminocaproic acid N-hydroxysuccinimide ester, is attached to the remaining amino group. A series of multiamine compouds, i. e. ethylendiamine, 1, 6-hexandediamine, triethylenetetramine, tetraethylenepentamine, and synthetic polyarylamide are used inlabelling, it is found that linker arms and steric hindrance effect the detection sensitivity. The probes labeled by 1, 6-hexandediamine and triethylenetetramine can detect 0.1-0.5pg target DNA in dot blot hybridization, and their sensitivities are better than the others.

本文详细考察了乙二胺、己二胺、二乙烯三胺、三乙烯四胺、四乙烯五胺及合成的聚乙烯胺化合物对λDNA探针的标记和检测的影响，发现胺化物链长和空间位阻影响探针的杂交和检测，用己二胺和三乙烯四胺标记的生物素探针在杂交24小时、显色8小时后检测灵敏度达0.1-0.5pg，超过其它胺化物标记的探针。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

There was no cross-reaction of the prepared raw antigen with the positive sera from dogs infected respectively with Ancylostoma caninum,Toxocara canis and Demodex canis and the highest titer of the positive serum from dogs infected with D.immitis was 1∶1024 detected by the dot-ELISA,which had good reproducibi-lity.

为快速诊断犬恶丝虫病，建立了犬恶丝虫抗体dot-ELISA检测方法，并与阻断试验、交叉反应试验、重复性试验、琼脂扩散试验和平行对照试验进行了比较。

The website that taught oneself dot ASP is made arrived in company of a network dawdle, had held out those who mix actually, basically seeing you is quite argute, the technology is bad to have nothing to do with, there is a program to be able to download on the net, need to alter, oneself won't be OK consult at the same time, but cannot let them see your what does not understand.

自学了点ASP的网站制作就到了一家网络公司里混日子，其实挺好混的，主要看你是不是够机灵，技术不好没关系，网上有程序可以去下载，需要改动的，自己不会可以请教同时，但是不能让他们看出来你啥都不懂。

A range of the autofrettage pressure permitted is presented and the best autofrettage pressure is decided according to the DOT CFFC.

最后，根据DOT CFFC标准确定出了预紧压力的范围，根据应力幅和平均应力确定出了最佳预紧压力。

Dot and Southern blotting analysis of transgenic individuals resistant to herbicide Basta indicated that all of these individuals contained T-DNA insertion.

转基因植株分子杂交检测结果与Basta抗性筛选的结果一致，确定了转基因植株的Basta筛选浓度与时期，提供了有效、方便、经济的大规模筛选水稻转化后代的方法。

One, look for what construction of accurate new rural area throws to cut a point according to Chongqing municipal government about building " on foot not wet shoe, cook not to burn bavin, water need not carry, building tile lid, village is beautiful rise " requirement of new rural area, those who combine Qi river county is actual, finance supports what new rural area builds to cut a dot to should try to consider from 5 respects.

一、找准新农村建设投入的切入点按照重庆市政府关于建设一个&走路不湿鞋、煮饭不烧柴，饮水不用抬、房屋砖瓦盖、村庄靓起来&的新农村要求，结合綦江县的实际，财政支持新农村建设的切入点应从五个方面加以考虑。

In this study,an experimental modelwas developed and established with freshwater crayfish Cambarus clarkii to prolif-erate White Spot Syndrome Virusin vivo.The success and reliability ofthis model was confirmed by transmission electron microscopy,dot-blotand hybridization in situ etc.

本研究在阐明WSSV对常见淡水甲壳纲动物的侵染活性的同时，成功地建立了WSSV淡水克氏螯虾体内增殖模型，并通过电镜技术、核酸探针斑点杂交与原位杂交等手段证实所建立的增殖模型的可靠性。

Cavelike dwellings built into soft rock dot the Cappadocia region of Turkey.

位于土耳其的似坑洞的住处建成到柔软的岩石圆点内Cappadocia的地区。

Cavelike dwellings built into soft rock dot the Cappadocia region of Turkey.

Cavelike居住群已经建筑在软岩石圆点的土耳其的卡帕多西亚地区。

If the truth is the rumours bring fluster, I would tell everybody the right information in time.

是谣言引起的慌乱，我会及时公布真实的信息。

Based on defining the concept of OAS, this dissertation first makes an analysis on the reasons and practical significance of OAS.

论文在对离岸资产证券化的概念进行界定的基础上，首先对离岸资产证券化的成因和现实意义进行了分析。