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For air shipments all packages must be incompliance with International Air Transport Association/International Civil Air Organization Dangerous Goods Regulations, as well as the DOT regulations.

对于空运，所有包装必须遵守国际航运组织/国际民航组织的危险货物规定，以及DOT的规范。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

The DoT effect will break roots , stuns , and mesmerization .

注意这个DOT将打断定身，打晕和催眠。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样，经微球菌溶解实验证明，3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L；从每组小鼠的12种组织提取总RNA，经Dot blotting检测证明，三种重组载体除在乳腺中表达外，p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达；在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

Requalification of cylinder shell and filler material can only be performed by a facility that has been authorized by and registered with DOT.

钢瓶瓶体和填充物材料的重新认定只能由已经在DOT注册并得到授权的机构进行。

DOT regulations require that the filler of all acetylene cylinders undergo a one-time inspection and requalification.

DOT规范要求所有的乙炔钢瓶的填充物要经过检查和重新认定。

Dot ELISA using 10～30 kDa antigen might be a specific and sensitive serodiagnostic method for diagnoing pagumogonimiasis.

斯氏狸殖吸虫成虫 10～ 30kDa抗原的dot ELISA为斯氏狸殖吸虫病高度特异和敏感的血清学诊断方法。

Is not half a minute before two of the 1000 injury to the other side of some blood DOT....

还不是那两个半分钟才伤害对方的1000来点血的DOT。。。。

You will get seduced , a DOT on him will prevent bandaging.

当你被CHARM后，敌人身上的DOT可以防止他给自己绷带。

With its reduced frameless design the shower cabin of IlBagnoAlessi dOt is not detracting from its original purpose the cleansing of body and soul.

IlBagnoAlessi dOt的简化框架设计的淋浴房没有失去他们的初衷，洗净身体和灵魂。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了