查询词典 dominant vector

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

The System inset remote sense vector data and GIS vector data in drainage area into client programs programmed, realizing the application of remote sense vector data and GIS vector data in water resources management of Xinjiang Ma-na-si River irrigation area, and realizing the integrated management of the texts and maps of the water resources survey data, and spatial analysis and map edit functions of GIS as well. The system, which breaks away the platform of MapInfo, can run independently.

该系统将流域中的遥感栅格数据与GIS矢量数据集成嵌入到所编制的客户程序中，实现遥感数据和GIS数据在新疆玛纳斯河流域水资源管理中的应用，和水资源调查数据图文一体化的管理，同时具备了GIS的空间分析和图形编辑等功能，该系统能脱离Mapinfo软件平台独立运行。

In chapter one, firstly, we introduce the properties of αdiagonally dominant matrix and some exists determination conditions of generalized strictly diagonally dominant matrix, then we give some new results for the criteria of generalized strictly diagonally dominant matrix, finally, we show the validity of these conclusions.

在第一章中，首先引述了α-对角占优矩阵的性质及已有的一些判定条件，给出了判定广义严格对角占优矩阵的几个新的结论，最后说明了这些结论的有效性。

Dominant industry concept is one of core categories with dominant industry academic choice, also be the theoretical foundation of strategy of dominant estate development.

主导产业概念是主导产业选择理论的核心范畴之一，也是主导产业发展战略的理论基础。

This research is to discuss the different effects dominant legs and non-dominant legs cause on static balance, ground reaction and speed by the following seven evaluation methods of dominant legs: ball-kick, step-up, hop-for-distance, step-forward, balance-recovery, fencing in epee, and individual preference.

摘要本研究的目的在利用七种惯用脚评定方式(踢球、登阶、跳远、往前踩步、恢复平衡、锐剑防卫和个人喜好等七种)，分别探讨惯用脚和非惯用脚在静态平衡能力、对地反作用力和速度之差异性，并发现六种评定方式的信度和七种惯用脚评定方式相互之间的差异性。

This paper starts on evocable disputes of price adjustment of commutation ticket of Nanjing public transportation. Then using the basic model of new economics of regulation, it analyzes the behavior selection of the government, regulatory agencies and the firms under regulation respectively in pricing game for public goods , and indicates that the dominant strategy of the dominant firms in the supplying public goods is to adjust the price instead of controlling cost, while other firms' dominant strategy is to follow.

本文以南京市公交月票的价格调整引起的争论为起点，根据新管制经济学的基本模型，分析在信息不对称的条件下政府、管制机构和被管制企业在公用产品价格博弈中的行为选择。

A clone from each genotype was randomly selected as representative for sequencing. The obtained 16S rDNA gene sequences had a similarity of 87%-100% with those in the GenBank (www. ncbi. nlm. nih. gov), and more than half of them had a similarity lower than 97%, being of new species. Based on phylogenetic analysis, the bacteria in the two soils were classified into 10 groups, with 5 groups in common. The dominant bacterial groups in the two soils differed obviously. In primeval forest soil, the dominant group was Proteobacteria, which had 39 genotypes, occupying 58.0% of all the clones; while in the soil of degraded ecosystem the dominant groups were Acidobacteria and Proteobacteria, which had 19 and 15 genotypes occupying 32.5% and 30.5% of all the clones, respectively. In the soil of degraded ecosystem, Proteobacteria group decreased while Acidobacteria group increased markedly, compared with those in primeval forest soil.

从每种基因型中随机选择一个克隆子作为代表进行测序分析，所有序列与GenBank数据库中序列的同源性为87%~100%，且两样地中均有超过一半的基因型序列与数据库中已知序列同源性低于97%，属于分类在"种"地位上的新发现细菌；通过系统发育研究将两样地的细菌分为10大类群，两样地共同拥有5大类群，但两样地的细菌优势类群明显不同，原生土壤为Proteobacteria，含39种基因型，占总克隆子数的58.0%，退化生态系统土壤为Acidobacteria和Proteobacteria，分别含19种和15种基因型，占总克隆子数的32.5%和30.5%；与原生土壤细菌类群相比，退化生态系统土壤Proteobacteria类群明显减少，Acidobacteria类群明显增加。

In encounters paired between unfamiliar males and males, there is great difference between dominant male and subordinate one. The dominants approached, attacked and chased the subordinate more often and spent significantly longer time.The subordinates defend or escaped the dominant more often and spent significantly longer time, but there is no significant difference on nonsocial behaviors between the dominant individual and the subordinate one.Frequencies of aggressive and social investigating behaviors are positively correlated with body mass.

结果发现，当陌生的两只雄鼠相遇时，优势雄鼠和从属雄鼠间的社会行为有明显的区别，优势鼠接近、攻击、追赶对方的频次和持续时间明显的多于从属鼠，而从属鼠逃离和防御行为多于优势鼠；优势鼠的体重明显的大于从属鼠，而且攻击行为和社会探究行为的频次与体重呈正相关，非社会行为无明显的差别。

The dominant point detection ability of the proposed planar contour dominant point detection method is tested by an emulational planar contour which includes all kinds of dominant points.

采用包含各种类型特征点的仿真平面轮廓，对本文提出的平面轮廓特征点检测方法的特征点检测能力进行了测试。

Researches reveal that students who suffer from corporal punishment tend to resort to violence in their future life.

研究表明遭受过肉体惩罚的孩子倾向于在他们今后的人生中使用暴力。

Total sugar of sarcocarp and chlorophyll of pericarp increased firstly, and then decreased afterward along with altitude; Titratable acidity, ascorbicacid and anthocyanin of pericarp were increased along with altitude; The content of protein increased firstly and decreased afterward along with altitude; Carotenoid of pericarp were decreased along with altitude.

果肉总糖和果皮叶绿素含量随海拔高度升高先升高后降低；可滴定酸、维生素C和果皮花青苷含量均随海拔升高而升高；蛋白质在1720m处最大，1420m处最小；果皮类胡萝卜素随海拔升高而降低。