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The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变;沉淀回收的PCR产物以20μl变性上样液(95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝)溶解后热变性为两条DNA单链后,上样于8%非变性聚丙烯酰胺凝胶(含5%甘油),700伏电压、4℃电泳16小时。

Methods:total alkaloids of radix Sophora lavescens were extracted by acidified water,then adjusted to neutral by sodium hydroxide solution, dissolved with buffer solution,then were mixed with 0.025% of bromothymol solution and chloroform,and then the mixture was shaken,standing and demixed,the absorbability of chloroform layer was detected at the wavelength of 413nm.

将苦参的酸水提取液用氢氧化钠溶液调节至中性后,依次加入pH=7.6缓冲溶液,溴代麝香草酚蓝指示液、氯仿混合振摇,静置分层,于413 nm波长处测定氯仿层吸收度。

So, the brucite dusts may react with and be dissolved partly in soluble ammonia salts of the human body and ammonia ions of body liquid.

因此,人体内的可溶性铵盐和体液中存在的铵离子可与水镁石粉尘作用并使之部分溶解。

To explore the effects of exogenous nitrogen input on the enzyme activity of Calamagrostis angustifolia during litter decomposition in a freshwater marsh, we supplied dissolved NH4NO3 (N0:0; N1:6 g·m-2·a-1;N2:12 g·m-2·a-1) to field plots on the Sanjiang Plain and determined the enzyme activities of litter decomposition within 160 d using litter bags.

以三江平原沼泽湿地典型湿地植物小叶章枯落物为研究对象,采用分解袋法研究了不同外源氮输入(N1、 N2分别为 6 、12g·m-2·a-1)对小叶章枯落物分解过程酶活性的影响,并设置对照区(N0,0 g· m-2·a-1)进行比较。

The main steps include as follows: CO2 is led into the mother liquor of sodium meta-aluminate to carry out carbon content decomposing; the primary separation between the aluminum and the gallium is realized by primary carbonatation to filter out sodium hydroxide; secondary carbonatation is carried out on the filtrate to obtain the deposit of the complex salt of gallium and aluminum; a sodium carbonate crystal is obtained after the filtered filtrate is condensed and vaporized; the deposit of the complex salt of gallium and aluminum is dissolved in the mother liquor of sodium meta-aluminate; the processes are repeated until the proportion of gallium and aluminum in the complex salt of gallium and aluminum is larger than 1/340; then the complex salt of gallium and aluminum is added into the NaOH liquor and electrolyzed, thus obtaining the metal gallium with a purity larger than 99.9 percent which reaches the level of 3; simultaneously, the byproducts of the aluminum hydroxide and the sodium carbonate during the process of carbonatation by steps are obtained.

主要步骤包括:向铝酸钠母液中通入CO 2 进行碳分分解,一次碳酸化实现铝镓的初次分离;过滤出氢氧化钠,滤液进行二次碳酸化获得镓铝复盐沉淀,过滤后滤液经浓缩蒸发获得碳酸钠晶体,铝镓复盐沉淀溶解在偏铝酸钠母液中,重复上述过程直至铝镓复盐中的镓铝比例大于1/340,将其加入到NaOH溶液中,电解可获得纯度>99.9%的金属镓,达到3N级水平同时在分步碳酸化的过程中获得氢氧化铝和碳酸钠副产品。

By preventing the passage of water through the cell walls of the endodermis, the Casparian strip forces it to pass through the cytoplasm, thus allowing selective uptake or exclusion of dissolved substances.

以阻止水分从内皮层细胞壁透过的方式,凯氏带迫使水分从细胞质中通过,继而可以从中选择性的吸收或排出可溶物质。

The effect of pre-polarization for Pt electrode on the behavior of anodic electrochemiluminescence of luminol in alkaline solution was studied. It was found that the Pt electrode cathodically prepolarized in alkaline solution with dissolved oxygen could enhance the ECL peak at about 0.22Vvs.

研究了铂电极的不同预极化处理过程对碱性鲁米诺阳极电致化学发光和阳极极化曲线的影响,发现在碱性含氧溶液中预还原处理的铂电极可增强0.22Vvs。

The possible mechanism for the two ECL channels at the Pt electrode cathodically pre-polarized in alkaline solution is also proposed:(1) luminol anion was electroxidized to radical at 0.22V, which then reacted with the oxygen dissolved in solution and emitted light;(2) the fresh oxygen species electro- generated at 1.07V oxidized luminol anion with light emission.

同时给出了在碱性含氧溶液中预还原的铂电极上两个可能的ECL反应通道:(1)在0.22V鲁米诺阴离子氧化为鲁米诺自由基,然后与溶解氧反应而发光;(2)1.07V处析出的新鲜氧与鲁米诺阴离子反应而发光。

The increase of fluorescence index and protein-like fluorescence signal suggested that bacteria could play an important role in the production of chromophoric dissolved organic matter.

荧光指数的增大与类蛋白荧光的产生,显示微生物在有色溶解有机质的生产上起着重要的作用。

A mysterious compound known as chromophoric dissolved organic matter may be a key piece in the puzzle of ocean life.

一种被称为有色可溶性有机物的神秘合成物也许会成为解决海洋生物问题的杀手锏。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。