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This investigation was to establish a simplified culture system to isolate and passage brain tumor stem cells from human medulloblastoma, observe their proliferation and differentiation, and determine the expression of normal neural stem cell antigens, CD133 and Nestin, in BTSCs. METHODS: Eleven specimens of medulloblastoma were acutely dissociated and triturated into single cells without trypsin digestion.

本研究旨在利用简化的无血清培养基和悬浮培养方法，从人髓母细胞瘤中分离培养脑肿瘤干细胞，观察其在体外的增殖、分化，鉴定其特异性标志物CD133和Nestin的表达，验证肿瘤组织切片中CD133阳性细胞的存在，为脑肿瘤干细胞的进一步研究打下基础。

The tissue was dissociated by digestion and triturating and made into single cell suspension, which was cultured in DMEM/F12 media containing 10% FBS, DMEM/F12 media containing 1% N2 supplement (N2 group), DMEM/F12 media containing 1%B27 supplement (B27 group) and DMEM/F12 media containing 1%N2 and 1% B27 supplements (N2+B27 group), the growth and purity of OECs were investigated.

方法分离新生2天SD大鼠的嗅球，制成单细胞悬液，在含10％FBS的DMEM／F12培养液、含1％N2的DMEM／F12培养液(N2组)、含1％B27的DMEM／F12培养液(B27组)以及含1％N2和1％B27的DMEM／F12培养液(N2+B27组)中进行培养，观察OECs的生长状态。

The adoption of baseplate with thick core would increase the release amount of dissociated P.F..

采用厚芯的基材，其释放量大；采用成熟的生产工艺，选择质量上乘的基材，可以减小释放量，但同时增加了成本。

To answer these questions, in the present study, an attempt was made to examine:(1) the GABA responses in mechanically dissociated Vme neurons with attached synaptic boutons by using the whole cell patch clamp technique;(2) the change of expression of KCC2 and NKCC1 during the postnatal stage in the Vme by using the Western blotting technique;(3) the percentage of colocalization of PV with KCC2 or NKCC1 during the postnatal stage in the Vme by using the double immunofluorescence histochemical techniques.

基于此，本论文进行了如下研究：(1)运用机械分离带终扣的单细胞膜片钳技术对大鼠Vme神经元对GABA的反应进行观察；(2)运用Western blotting技术结合图像分析方法观察生后发育过程中Vme神经元内NKCC1和KCC2的表达变化；(3)运用免疫荧光组织化学双重标记技术对生后发育过程中Vme神经元内NKCC1和KCC2与PV之间的共存关系进行了系统观察。

Cerebral cortexes were removed and then dissociated, measured with flow cytometer analyze cell apoptosis, the morphological changes were examined by electron microscope.(2) Measured cell apoptosis of cerebral cortexes with TUNEL.(3) detected expressions of Bcl-2, Bax and Caspase-3 proteins with the technique of immunohistochemistry.

1取大脑皮层细胞制成细胞悬液，流式细胞仪分析细胞凋亡改变，电镜观察细胞超微结构的变化；(2)用原位缺口末端标记法检测凋亡细胞百分率；(3)免疫组化法检测Bcl-2、Bax和Caspase-3蛋白表达。

Dissociated cells were plated onto poly-d-lysine-coated coverslips and propagated in medium containing recombined Wnt3a-adenovirus. Plenty of Nurr1 were detected by RT-PCR after 3 days.

将神经干细胞分为4组，对照组、抗坏血酸诱导组、Wnt3a重组腺病毒诱导组（Wnt3a组）以及Wnt3a重组腺病毒加抗坏血酸诱导组（Wnt3a+AA组）。

In the presence of high concentration of detergent DOC, the higher-order form dissociated to dimers.

此higher-order结构在高浓度清洁剂DOC下，会发生解离，成为dimer型式。

These photo generated excitons can be dissociated into free charge carriers by built in field at the organi.

这些激子被有机／有机界面的内建场离解成自由载流子。

The result also suggested that the Rubisco holoenzyme had not dissociated when LSU hydrolyzed from 53kD to 50kD.

进一步的研究发现LSU发生这步裂解时，Rubisco全酶没有解离，50kD降解产物仍能组装在Rubisco全酶中。

The retinal of 18 post-natal 2-3 days Sprague-Dawley rats were dissociated into cell suspension with trypsin digestion. The cell suspension was implated in 96-well culture plates covered with hyaluronic acid and laminin in each well.

采用胰酶消化法将18只出生2-3d的乳鼠视网膜制成细胞悬液，接种于经多聚鸟氨酸和层粘连蛋白包被的96孔板中。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。