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With the increasing of stress and incensitive rearing, change of rearing method and high rearing density, Mycoplasma Gallisepticum infection have already been a major problem that affects the poultry industry through its increased morbidity. MG infection can be easily triggered under such environment as poor ventilation , a sudden air temperature change and excessive ammonia and dusts ,even after vaccinating the live vaccine such as Newcastle disease, Infectious bronchitis and Infectious Laryngotracheitis can also triggered this disease.

随着养鸡集约化程度的提高,饲养方式的改变以及饲养密度的提高,在通风不良、空气中氨和灰尘增加或气温骤变等环境中极易诱发本病;甚至接种鸡新城疫、传染性支气管炎和传染性喉气管炎等活疫苗后也都可能诱发本病。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

The thesis decribes two methods of detecting CLCuV-PK: TAS-ELISA and PCR. it is shown that CLCuV-PK is very closely related to viruses causing yellow vein disease or leaf curl disease of okra in Pakistan.

本文用二种方法检测CLCuV-PK,证明CLCuV-PK与引起巴基斯坦秋葵曲叶和秋葵黄化叶脉花叶的病毒有很近的亲缘关系。

The results indicated that the incidence of virus disease in processed tomato was 63%~91% from 2005 to 2006. The field symptoms had various disease symptoms including mosaic, leaf streak necrosis, fernbrake leaf and malformation, curl leaf and dwarf etc., but mainly mosaic and leaf streak necrosis with infectious frequencies of 78% and 37%, and leaf streak necrosis symptom with mosaic was above 90%.

结果表明:加工番茄病毒病害2005~2006年间各地发病率达63%~91%,在田间主要表现为花叶、条斑坏死、蕨叶畸形、曲叶和矮化等多种病害症状,以花叶和坏死条斑为主,其中花叶占78%,条斑占37%,并且条斑症状90%以上都伴有花叶症状。

Leishmania infection can produce ulcers, skin disease and also cause systemic disease .

利什曼原虫感染可产生溃疡,皮肤疾病,也cause 全身性疾病。

Br environmental pollution already arouses 35.7%'s pneogaster disease , 22%'s chronic pulmonary tuberculosis composes in reply 15%'s tracheitis , English disease with lung cancer , leucocythemia.

环境污染已经引起35.7的呼吸道疾病,22的慢性肺病和15的气管炎、支气管炎和肺癌,白血病。

Results Among 234 cases,38 patients (16.2%) had complicated with autoimmune diseases,which usually were rheumatoid arthritis (31.6%) and ulcerative colitis (26.3%). The rate of transforming to leucocythemia in MDS patients complicated with autoimmune disease is not more than that in MDS patients without autoimmune disease,but the median lifetime decreased.

结果 234例MDS患者中38例并发自身免疫性疾病,占162%;MDS并发自身免疫性疾病以类风湿关节炎(316%)和溃疡性结肠炎(263%)常见;MDS并发自身免疫性疾病者比无自身免疫性疾病者其转白率并不增加,但中位生存期缩短。

Br boundary contaminates the pneogaster disease already arousing 35.7%, 22%'s chronic pulmonary tuberculosis composes in reply 15%'s tracheitis , English disease with lung cancer , leucocythemia.

境污染已经引起35.7的呼吸道疾病,22的慢性肺病和15的气管炎、支气管炎和肺癌,白血病。

Furthermore,conditions for the uniform persistence of disease and conditions for global stability of the disease-free equi- librium and the endemic equilibrium are obtained by Liapunov method and by analyzing the property of flow on the boundary,respectively.

进一步讨论了疾病持续存在与无病平衡点和地方病平衡点全局稳定的条件。

According to the report, patient of our country coronary heart disease metabolization of 80% existence candy is unusual, but the research report that waits for chronic illness influence to hypertensive, fatty liver, coronary heart disease, tall lipemia about tall blood sugar is less.

据报道,我国冠心病患者80%存在糖代谢异常,但有关高血糖对高血压、脂肪肝、冠心病、高脂血症等慢性疾病影响的探究报道较少。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。