查询词典 direct method

PCRs were performed on genomic DNAs of four pear cultivars Sand pear, Huangguan pear, Xueli pear, Yali pear using primers derived from sequences of previous published PPO. The target bands were recovered and purified. The fragments were ligated with the plasmid of pGEM-T Vector by the method of direct T-A cloning.

以4种不同来源的梨基因组DNA为模板，根据已经发表的多酚氧化酶基因序列设计引物，利用PCR技术，克隆到了鸭梨、雪花梨、砂梨、黄冠梨约1.8 kb的完整多酚氧化酶基因，将纯化后的扩增产物克隆到质粒pGEM-T vec-tor中，转化DH-5α菌，挑选阳性克隆进行测序。

The Forth, the direct willingness survey method is used to identify and measure credit demand and credit constraint which is self-containedly categorized, in which repayment capacity, other credit sector and measurement errors are controlled.

第四，在信贷需求与信贷约束的识别上，采用直接意愿调查方法识别出了具有完备分类特征的信贷需求与信贷约束类型，并且，在调查过程中控制住还款能力、其他贷款渠道及测量误差等因素对调查的干扰。

The measuring of self-inductance coefficient by means of direct current bridge method has reduced the difficult and improved the precision of measuring.

传统测量电感的自感系数是用交流电桥，它存在平衡点较难找，并且在空间杂散信号干扰下很容易产生误差。

Based on semitransparent effect,direct volume rendering method can display the small and poorly defined features of the 3D dataset.

直接体绘制技术利用半透明效果能够在一定程度上绘制三维数据场的精细结构。

Hierarchical Indexed Sequential Direct Access Method?

分层次索引顺序直接存取法

By using the direct parallel method, the optimal control problem is converted into a nonlinear programming problem that is then solved by means of the sequential quadratic programming.

用直接配置方法将最优控制问题转化为非线性规划问题，用序列二次规划方法对转化后的非线性规划问题进行求解。

And can also follow the magnitude of the direct stress and shearing stress ,the method can be used to effectively simulate the continuum cracking and sliding on the interface .

并且还可以根据所求正应力和剪应力的大小，很方便地模拟坝体层面的连续、滑移以及开裂等工作状态。

In order to probe the absorb coefficients of earth medium by the direct wave, reflected wave and transmied wave amplititude which are picked up from the radar wave racords yielded by finite element method,the paper gives out the attenuated radar wave equations in time domain and the attenuated radar wave differential equations while the motion changes simple harmonic with time and electric parametets change slowly with space.

摘 要 为了用有限元方法生成雷波正演记录，从而拾取直达波、反射波、透射波振幅信息，以确定介质吸收系数，文章在时间域给出了雷达波含衰减项的波动方程，及在运动随时间简谐变化以及电性参数随空间缓变的条件含衰减项的雷达波遵循的简化微分方程。

The high order single step algorithm is a new method of direct integration for solution equations of motion.

非线性结构时程分析的高阶单步算法是一种新的求解运动方程的直接积分法。

This resulted in an MISPE-HPLC method allowing the direct extraction of the analytes from the sample matrix with a selectie wash using just 10% organic solent.

这样就产生了一种MISPE-HPLC方法，可以用仅含10%有机溶剂的选择性洗液从样品基质中直接萃取被分析物。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。