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A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Result The highest level of growth factor 61.1μg/L,was produced by trichophyton rubrum(turbidimetry 4.5×10 10 ),and the strongest effect was at the point of double diluted filtrarate,cell index reached 2800 cpm/dis...

结果 红色毛癣菌分泌EFG最高 6 1 。1 μg/L(浊度比 4 。5× 1 0 10 )，对倍稀释液对培养的原代人类角质形成细胞作用最强，角质形成细胞标记指数可达 2 80 0cpm/盘。

This article brings forward the technology and method of extracting xylose from the black corncon by natural zymotechnics and hydrolyzati unden the condition of diluted acid and ordinary pressure.

提出了用自然发酵和稀酸常压水解法从黑玉米芯中提取木糖的工艺和方法，通过红外光谱和紫外光谱确证结构，其产率达到28.65%，并讨论了影响木糖提取率的因素。

They were equal to or better than the results of 50% kangyawei wettable powder diluted with 2500 water.

百部·楝·烟乳油是由海南侨华农药厂生产的一种新型农药，其毒

When they were diluted to 1%, the eye irritation of acet, alcohol and DMSO were little, while methanol had eye irritation still.

当稀释为1%后，丙酮、乙醇、二甲基亚砜无眼刺激性，但甲醇刺激性仍然较高。

Alkalinized by natrium hydroxydatum and diluted to measure concentration of uric acid.

收集结石患者24h尿液，用浓盐酸酸化和稀释处理后，用生化分析仪测定其中钙、磷浓度，再以氢氧化钠碱化和稀释后测定尿酸浓度。

With the life bettered and levels of education higher, the work ethic is diluted now

随着生活的改善和教育水平的提高，职业道德的观念遭到了削弱。

Methods Ninety ASA Ⅰ~Ⅱ patients undergoing UPPP surgery were randomly divided into three groups: F group (fentanyl 10 μg/kg), FL group (fentanyl 5 μg/kg + lornoxicam 0.4 mg/kg), or TL group (tramadol 5 mg/kg + lornoxicam 0.4 mg/kg). The drugs were diluted with normal saline to 100 mL (background 2 mL/h, PCIA bolos 1.5 mL, lockout time 15 min).

90例择期行UPPP的阻塞性睡眠呼吸暂停综合征（obstructive sleep apnea， syndrome， OSAS，即鼾症）患者，ASAⅠ~Ⅱ级，随机分为3组(n=30)，F组：芬太尼10μg/kg；FL组：芬太尼5μg/kg+氯诺昔康0.4mg/kg；TL组：曲马多5mg/kg+氯诺昔康0.4mg/kg；每组镇吐药物均为氟哌啶5mg，均以生理盐水稀释至100mL，背景剂量2mL/h，PCIA1.5 mL/次，锁定时间15min。

When their fermentation broths were diluted 5-fold, the strains DA07041 and DA07118 had higher knockdown efficiency, respectively 72.1%,and 83.7%.

发酵液稀释5倍后对线虫的击倒效果最好的是：DA07041和DA07118，其校正击倒率分别为72.1%和83.7%。

Commercial humic acid is diluted with different quantity of deionized water as water sample of known DOM concentration.

用商品腐殖酸和去离子水配置成已知浓度的溶液代替标准DOM溶液进行标定，得到回归方程。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。